Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid
Carregando...
Citações na Scopus
18
Tipo de produção
article
Data de publicação
2020
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
ALMEIDA, Roque Pacheco de
FORTALEZA, Carlos Magno Castelo Branco
DRUZIAN, Angelita Fernandes
LEMOS, Ana Priscila Freitas
MELO, Vanessa Campos Andrade de
PANIAGO, Anamaria Mello Miranda
Citação
PLOS ONE, v.15, n.4, article ID e0230610, 19p, 2020
Resumo
Background The development of rK39-based immunochromatographic rapid diagnostic tests represents an important advance for serodiagnosis of visceral leishmaniasis, being cheap and easy to use at the point of care (POC). Although the use of rK39 have considerably improved the sensitivity and specificity of serological tests compared with total antigens, great variability in sensitivity and specificity was reported. This study aimed at the evaluation of ""Kalazar Detect (TM) Rapid Test, Whole Blood"" (Kalazar Detect RDT) for Visceral Leishmaniasis (VL) diagnosis using oral fluid, whole blood and serum specimens collected at different endemic areas of VL of Brazil. Methodology To evaluate Kalazar Detect RDT, oral fluid, whole blood and serum specimens from 128 VL patients, 85 healthy individuals, 22 patients with possible cross-reactivity diseases and 20 VL/aids coinfected patients were collected and assayed at the POC. Principal findings and conclusions The performance of Kalazar Detect RDT in whole blood and serum was similar; however, using oral fluid, the sensitivity was low. Particularly in samples from the city of Natal, Rio Grande do Norte state in Northeastern Brazil, we observed low sensitivity, 80.0% (95% CI: 62.7-90.5), using whole blood and serum, and poor sensitivity, 43.3% (95% CI: 27.4-60.8) with oral fluid. Those values were much lower than in the other regions, where sensitivity ranged from 92.7-96.3% in whole blood and serum, and 80.0-88.9% in oral fluid. Besides, in VL/aids coinfected patients, lower sensitivity was achieved compared with VL patients. In samples from Natal, the sensitivity was 0.0% (95% CI: 0.0-49.0) and 25.0% (95% CI: 4.6-69.9), using oral fluid and serum/whole blood, respectively; in samples from the other regions, the sensitivity ranged from 40.0-63.6% and 80.0-81.8%, respectively. As for specificity, high values were observed across the fluids, 100.0% (95% CI: 96.5-100.0) in whole blood, 96.3% (95% CI: 90.8-98.5) in serum, and 95.3% (95% CI: 89.5-98.0) in oral fluid; across localities, specificity ranged from 85.7-100.0%. Serum samples sent by the collaborating centers to Instituto de Medicina Tropical (n = 250) were tested by Kalazar Detect RDT, Direct Agglutination Test, Indirect immunofluorescence assay, Enzyme-linked immunosorbent assay, and IT-Leish (R) RDT. The regional difference in the performance of rK39-based RDT and lower sensitivity in Leishmania/HIV coinfected patients raise concern on the routine use of these products for the diagnosis of VL.
Palavras-chave
Referências
- Alvar J, 2004, ADV PARASIT, V57, P1, DOI 10.1016/S0065-308X(04)57001-X
- [Anonymous], 2019, LEISHMANIOSE VISCERA
- [Anonymous], 2000, LETALIDADE LEISHMANI
- [Anonymous], 2019, CASOS CONFIRMADOS LE
- Attia J, 2002, AUSTR PRESCRIBER, P26
- Bangert M, 2018, PLOS NEGLECT TROP D, V12, DOI 10.1371/journal.pntd.0006277
- Banoo S, 2010, NAT REV MICROBIOL, pS17, DOI [10.1038/nrmicro1523, 10.1038/nrmico1523]
- Bhattacharyya T, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002057
- da Silva MRB, 2018, PARASITOL RES, V117, P419, DOI 10.1007/s00436-017-5716-3
- Boelaert M, 2007, NATURE REV MICROBIOL, V5, pS30, DOI 10.1038/NRMICRO1766
- BURNS JM, 1993, P NATL ACAD SCI USA, V90, P775, DOI 10.1073/pnas.90.2.775
- Celeste Beatriz Julieta, 1998, Revista do Instituto de Medicina Tropical de Sao Paulo, V40, P287, DOI 10.1590/S0036-46651998000500004
- Chappuis F, 2006, TROP MED INT HEALTH, V11, P31, DOI 10.1111/j.1365-3156.2005.01533.x
- Chappuis F, 2005, J CLIN MICROBIOL, V43, P5973, DOI 10.1128/JCM.43.12.5973-5977.2005
- Cota GF, 2013, AM J TROP MED HYG, V89, P570, DOI 10.4269/ajtmh.13-0239
- Cunningham J, 2012, CLIN INFECT DIS, V55, P1312, DOI 10.1093/cid/cis716
- da Silva MRB, 2015, TROP BIOMED, V32, P247
- Deeks JJ, 2004, BRIT MED J, V329, P168, DOI 10.1136/bmj.329.7458.168
- ELHARITH A, 1988, J CLIN MICROBIOL, V26, P1321, DOI 10.1128/JCM.26.7.1321-1325.1988
- Fleiss JL, 2003, STAT METHODS RATES P
- Freire ML, 2019, PLOS NEGLECT TROP D, V13, DOI 10.1371/journal.pntd.0007484
- Furukawa TA, 2008, USERS GUIDES MED LIT, P195
- Galai Y, 2011, J CLIN MICROBIOL, V49, P3150, DOI 10.1128/JCM.00267-11
- Grimes DA, 2005, LANCET, V365, P1500, DOI 10.1016/S0140-6736(05)66422-7
- Grimes DA, 2002, LANCET, V359, P881, DOI 10.1016/S0140-6736(02)07948-5
- GUIMARAES MCS, 1989, B WORLD HEALTH ORGAN, V67, P643
- Herwaldt BL, 1999, LANCET, V354, P1191, DOI 10.1016/S0140-6736(98)10178-2
- LANDIS JR, 1977, BIOMETRICS, V33, P363, DOI 10.2307/2529786
- Lindoso JAL, 2012, RES REP TROP MED, V3, P69, DOI 10.2147/RRTM.S24764
- Maia Z, 2012, PLOS NEGLECT TROP D, V6, DOI 10.1371/journal.pntd.0001484
- Ferreira GEM, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0036242
- Mikaeili Fattaneh, 2007, Iran J Immunol, V4, P116
- Mohapatra S, 2016, J ARTHROPOD-BORNE DI, V10, P87
- Motoie G, 2013, INFECT GENET EVOL, V18, P48, DOI 10.1016/j.meegid.2013.04.031
- Pedras MJ, 2008, T ROY SOC TROP MED H, V102, P172, DOI 10.1016/j.trstmh.2007.11.004
- Peruhype-Magalhaes V, 2012, MEM I OSWALDO CRUZ, V107, P951, DOI 10.1590/S0074-02762012000700019
- Ritmeijer K, 2006, AM J TROP MED HYG, V74, P76, DOI 10.4269/ajtmh.2006.74.76
- SIMEL DL, 1991, J CLIN EPIDEMIOL, V44, P763, DOI 10.1016/0895-4356(91)90128-V
- Singh DP, 2010, J HEALTH POPUL NUTR, V28, P281
- Srivastava P, 2011, T ROY SOC TROP MED H, V105, P1, DOI 10.1016/j.trstmh.2010.09.006
- Srividya G, 2012, PARASITOL RES, V110, P1065, DOI 10.1007/s00436-011-2680-1
- Sundar S, 1998, LANCET, V351, P563, DOI 10.1016/S0140-6736(97)04350-X
- Vaish M, 2012, AM J TROP MED HYG, V86, P598, DOI 10.4269/ajtmh.2012.11-0127
- van Griensven J, 2019, INFECT DIS CLIN N AM, V33, P79, DOI 10.1016/j.idc.2018.10.005
- World Health Organization, 2019, LEISHMANIASIS
Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/38
Artigos e Materiais de Revistas Científicas - LIM/49
Artigos e Materiais de Revistas Científicas - ODS/03
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/38
Artigos e Materiais de Revistas Científicas - LIM/49
Artigos e Materiais de Revistas Científicas - ODS/03