Simultaneous Quantification of Lycopene, beta-Carotene, Retinol and alpha-Tocopherol in Plasma after a Simple Extraction Procedure: Stability Study and Application to Human Volunteers

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCHARAO, Mariele F.
dc.contributor.authorMORO, Angela M.
dc.contributor.authorBRUCKER, Natalia
dc.contributor.authorBULCAO, Rachel P.
dc.contributor.authorBAIERLE, Marilia
dc.contributor.authorFREITAS, Fernando
dc.contributor.authorDURGANTE, Juliano
dc.contributor.authorNASCIMENTO, Sabrina
dc.contributor.authorBUBOLS, Guilherme B.
dc.contributor.authorSALDIVA, Paulo H.
dc.contributor.authorBOHRERE, Denise
dc.contributor.authorGARCIA, Solange C.
dc.date.accessioned2013-07-30T15:14:54Z
dc.date.available2013-07-30T15:14:54Z
dc.date.issued2012
dc.description.abstractA method for the simultaneous quantification of lycopene, beta-carotene, retinol and alpha-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20 degrees C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra-and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20 degrees C. A significant decrease of lycopene, beta-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.
dc.description.abstractUm método para quantificação simultânea de licopeno, β-caroteno, retinol e α-tocoferol por cromatografia líquida de alta eficiência (HPLC) com detecção no visível/fluorescente e eluição isocrática foi otimizado e validado. O método consiste de extração líquido-líquido rápida e simples e posterior quantificação do sobrenadante extraído por HPLC. Alíquotas de plasma foram estocadas a –20°C por três meses para estudo da estabilidade. Aplicação metodológica foi realizada em amostras fornecidas por pintores e indivíduos não expostos a tintas (n = 75). O ensaio foi linear para todas as vitaminas analisadas (r > 0,99). Precisões intradia e interdia apresentaram coeficiente de variação (CV) menor que 5%. Exatidões variaram de 0,29 a –5,80% e recuperações entre 92,73 e 101,97%. Amostras de plasma e sobrenadante extraído foram estáveis por até 60 dias a –20°C. Foi demonstrada uma diminuição significativa nas concentrações de licopeno, β-caroteno e retinol em indivíduos expostos quando comparados com os não-expostos (p < 0,05). O método é simples, reprodutível, preciso, exato e sensível, e pode ser utilizado na rotina de laboratórios clínicos.
dc.description.indexPubMed
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior [CAPES/DAAD PROBRAL 352/10]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq/MCT) [479613/2009-5]
dc.description.sponsorshipCAPES/REUNI
dc.description.sponsorshipCNPq
dc.identifier.citationJOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, v.23, n.8, p.1441-1449, 2012
dc.identifier.doi10.1590/S0103-50532012005000001
dc.identifier.issn0103-5053
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/942
dc.language.isoeng
dc.publisherSOC BRASILEIRA QUIMICA
dc.relation.ispartofJournal of the Brazilian Chemical Society
dc.rightsopenAccess
dc.rights.holderCopyright SOC BRASILEIRA QUIMICA
dc.subjectretinol
dc.subjectalpha-tocopherol
dc.subjectlycopene
dc.subjectbeta-carotene
dc.subjectHPLC
dc.subjectliquid-liquid extraction
dc.subject.otherperformance liquid-chromatography
dc.subject.otherhuman serum
dc.subject.otherelectrochemical detection
dc.subject.othervitamin-a
dc.subject.othervalidation
dc.subject.otherantioxidants
dc.subject.otherstandard
dc.subject.wosChemistry, Multidisciplinary
dc.titleSimultaneous Quantification of Lycopene, beta-Carotene, Retinol and alpha-Tocopherol in Plasma after a Simple Extraction Procedure: Stability Study and Application to Human Volunteers
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalCHARAO, Mariele F.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Santa Maria, Ctr Hlth Sci, Postgrad Program Pharmacol, BR-97105900 Santa Maria, RS, Brazil
hcfmusp.author.externalMORO, Angela M.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalBRUCKER, Natalia:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalBULCAO, Rachel P.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalBAIERLE, Marilia:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalFREITAS, Fernando:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalDURGANTE, Juliano:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalNASCIMENTO, Sabrina:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalBUBOLS, Guilherme B.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.author.externalBOHRERE, Denise:Univ Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil
hcfmusp.author.externalGARCIA, Solange C.:Univ Fed Rio Grande do Sul, Dept Anal, Lab Toxicol, BR-90610000 Porto Alegre, RS, Brazil; Univ Fed Rio Grande do Sul, Postgrad Program Pharmaceut Sci, BR-90610000 Porto Alegre, RS, Brazil
hcfmusp.citation.scopus10
hcfmusp.contributor.author-fmusphcPAULO HILARIO NASCIMENTO SALDIVA
hcfmusp.description.beginpage1441
hcfmusp.description.endpage1449
hcfmusp.description.issue8
hcfmusp.description.volume23
hcfmusp.origemWOS
hcfmusp.origem.scieloSCIELO:S0103-50532012000800006
hcfmusp.origem.scopus2-s2.0-84866407917
hcfmusp.origem.wosWOS:000309162200006
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.remissive.sponsorshipCAPES
hcfmusp.remissive.sponsorshipCNPq
hcfmusp.remissive.sponsorshipCAPES
hcfmusp.remissive.sponsorshipCNPq
hcfmusp.remissive.sponsorshipDAAD
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