Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorJUN, Jonathan C.
dc.contributor.authorSHIN, Mi-Kyung
dc.contributor.authorYAO, Qiaoling
dc.contributor.authorBEVANS-FONTI, Shannon
dc.contributor.authorPOOLE, James
dc.contributor.authorDRAGER, Luciano F.
dc.contributor.authorPOLOTSKY, Vsevolod Y.
dc.date.accessioned2013-07-30T17:52:32Z
dc.date.available2013-07-30T17:52:32Z
dc.date.issued2012
dc.description.abstractObstructive sleep apnea (OSA) induces intermittent hypoxia (IH) during sleep and is associated with elevated triglycerides (TG). We previously demonstrated that mice exposed to chronic IH develop elevated TG. We now hypothesize that a single exposure to acute hypoxia also increases TG due to the stimulation of free fatty acid (FFA) mobilization from white adipose tissue (WAT), resulting in increased hepatic TG synthesis and secretion. Male C57BL6/J mice were exposed to FiO(2) = 0.21, 0.17, 0.14, 0.10, or 0.07 for 6 h followed by assessment of plasma and liver TG, glucose, FFA, ketones, glycerol, and catecholamines. Hypoxia dose-dependently increased plasma TG, with levels peaking at FiO(2) = 0.07. Hepatic TG levels also increased with hypoxia, peaking at FiO(2) = 0.10. Plasma catecholamines also increased inversely with FiO(2). Plasma ketones, glycerol, and FFA levels were more variable, with different degrees of hypoxia inducing WAT lipolysis and ketosis. FiO(2) = 0.10 exposure stimulated WAT lipolysis but decreased the rate of hepatic TG secretion. This degree of hypoxia rapidly and reversibly delayed TG clearance while decreasing [H-3]triolein-labeled Intralipid uptake in brown adipose tissue and WAT. Hypoxia decreased adipose tissue lipoprotein lipase (LPL) activity in brown adipose tissue and WAT. In addition, hypoxia decreased the transcription of LPL, peroxisome proliferator-activated receptor-gamma, and fatty acid transporter CD36. We conclude that acute hypoxia increases plasma TG due to decreased tissue uptake, not increased hepatic TG secretion.
dc.description.indexMEDLINE
dc.description.sponsorshipEudowood Foundation [80026999]
dc.description.sponsorshipJohns Hopkins Clinician Scientist Award [80028014]
dc.description.sponsorshipNIH/National Heart, Lung, and Blood Institute [T32 HL-07534, R01 HL-080105, P50 HL-084945]
dc.description.sponsorshipAmerican Lung Association-National Sleep Foundation Pickwick Fellowship [SF-78568-N]
dc.description.sponsorshipAmerican Heart Association [10GRNT3360001]
dc.identifier.citationAMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v.303, n.3, p.E377-E388, 2012
dc.identifier.doi10.1152/ajpendo.00641.2011
dc.identifier.issn0193-1849
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/1482
dc.language.isoeng
dc.publisherAMER PHYSIOLOGICAL SOC
dc.relation.ispartofAmerican Journal of Physiology-Endocrinology and Metabolism
dc.rightsrestrictedAccess
dc.rights.holderCopyright AMER PHYSIOLOGICAL SOC
dc.subjectlipolysis
dc.subjectlipases
dc.subjectadipose
dc.subjectthermoregulation
dc.subjectmetabolism
dc.subject.otherchronic intermittent hypoxia
dc.subject.otherobstructive sleep-apnea
dc.subject.otherhigh-altitude exposure
dc.subject.otheractivated protein-kinase
dc.subject.otherhormone-sensitive lipase
dc.subject.otherbrown adipose-tissue
dc.subject.otherlipoprotein-lipase
dc.subject.othergene-expression
dc.subject.otherlipid-metabolism
dc.subject.otheradipocyte differentiation
dc.subject.wosEndocrinology & Metabolism
dc.subject.wosPhysiology
dc.titleAcute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalJUN, Jonathan C.:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.author.externalSHIN, Mi-Kyung:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.author.externalYAO, Qiaoling:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.author.externalBEVANS-FONTI, Shannon:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.author.externalPOOLE, James:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.author.externalPOLOTSKY, Vsevolod Y.:Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD 21224 USA
hcfmusp.citation.scopus68
hcfmusp.contributor.author-fmusphcLUCIANO FERREIRA DRAGER
hcfmusp.description.beginpageE377
hcfmusp.description.endpageE388
hcfmusp.description.issue3
hcfmusp.description.volume303
hcfmusp.lim.ref2012
hcfmusp.origemWOS
hcfmusp.origem.pubmed22621867
hcfmusp.origem.scopus2-s2.0-84864522443
hcfmusp.origem.wosWOS:000307228700008
hcfmusp.publisher.cityBETHESDA
hcfmusp.publisher.countryUSA
hcfmusp.relation.referenceDrager LF, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012065
hcfmusp.relation.referenceBallart X, 2003, BIOCHIMIE, V85, P971, DOI 10.1016/j.biochi.2003.09.001
hcfmusp.relation.referenceBarnholt KE, 2006, AM J PHYSIOL-ENDOC M, V290, pE1078, DOI 10.1152/ajpendo.00449.2005
hcfmusp.relation.referenceBartelt A, 2011, NAT MED, V17, P200, DOI 10.1038/nm.2297
hcfmusp.relation.referenceBAUM D, 1967, P SOC EXP BIOL MED, V125, P1190
hcfmusp.relation.referenceBAUM D, 1971, AM J DIS CHILD, V121, P115
hcfmusp.relation.referenceBAUM D, 1981, AM J PHYSIOL, V241, pE28
hcfmusp.relation.referenceBeigneux AP, 2009, J LIPID RES, V50, pS57, DOI 10.1194/jlr.R800030-JLR200
hcfmusp.relation.referenceBligh G, 1959, CAN J BIOCH PHYSL, V37, P911
hcfmusp.relation.referenceBRINDLE NPJ, 1988, BIOCHEM J, V250, P363
hcfmusp.relation.referenceBrito J, 2007, HIGH ALT MED BIOL, V8, P236, DOI 10.1089/ham.2007.8310
hcfmusp.relation.referenceCannon B, 2004, PHYSIOL REV, V84, P277, DOI 10.1152/physrev.00015.2003
hcfmusp.relation.referenceCarriere A, 2004, J BIOL CHEM, V279, P40462, DOI 10.1074/jbc.M407258200
hcfmusp.relation.referenceCHAIT A, 1979, METABOLISM, V28, P553, DOI 10.1016/0026-0495(79)90197-5
hcfmusp.relation.referenceCHIODI H., 1952, ACTA PHYSIOL LATINOAMER, V2, P228
hcfmusp.relation.referenceDavidson NO, 2000, ANNU REV NUTR, V20, P169, DOI 10.1146/annurev.nutr.20.1.169
hcfmusp.relation.referenceDrager LF, 2012, EUR HEART J, V33, P783, DOI 10.1093/eurheartj/ehr097
hcfmusp.relation.referenceDrager LF, 2010, CURR OPIN ENDOCRINOL, V17, P161, DOI 10.1097/MED.0b013e3283373624
hcfmusp.relation.referenceFarias JG, 2006, HIGH ALT MED BIOL, V7, P302, DOI 10.1089/ham.2006.7.302
hcfmusp.relation.referenceFISER RH, 1974, J NUTR, V104, P223
hcfmusp.relation.referenceFrayn KN, 2010, ACTA PHYSIOL, V199, P509, DOI 10.1111/j.1748-1716.2010.02128.x
hcfmusp.relation.referenceFRAYN KN, 1995, ADV ENZYME REGUL, V35, P163, DOI 10.1016/0065-2571(94)00011-Q
hcfmusp.relation.referenceFREDHOLM B B, 1970, Acta Physiologica Scandinavica, V80, P567, DOI 10.1111/j.1748-1716.1970.tb04824.x
hcfmusp.relation.referenceFRIED SK, 1987, INT J OBESITY, V11, P129
hcfmusp.relation.referenceGauthier MS, 2008, J BIOL CHEM, V283, P16514, DOI 10.1074/jbc.M708177200
hcfmusp.relation.referenceGautier H, 1996, J APPL PHYSIOL, V81, P521
hcfmusp.relation.referenceGoldberg IJ, 2009, J LIPID RES, V50, pS86, DOI 10.1194/jlr.R800085-JLR200
hcfmusp.relation.referenceGoudriaan JR, 2005, J LIPID RES, V46, P2175, DOI 10.1194/jlr.M500112-JLR200
hcfmusp.relation.referenceHavel R, J LIPID RES, V1, P1959
hcfmusp.relation.referenceHILL JR, 1959, J PHYSIOL-LONDON, V149, P346
hcfmusp.relation.referenceJun J, 2010, ATHEROSCLEROSIS, V209, P381, DOI 10.1016/j.atherosclerosis.2009.10.017
hcfmusp.relation.referenceJun J, 2009, ILAR J, V50, P289
hcfmusp.relation.referenceKahn BB, 2005, CELL METAB, V1, P15, DOI 10.1016/j.cmet.2004.12.003
hcfmusp.relation.referenceKAYSER B, 1992, INT J SPORTS MED, V13, pS129, DOI 10.1055/s-2007-1024616
hcfmusp.relation.referenceKim KH, 2005, BIOCHEM BIOPH RES CO, V333, P1178, DOI 10.1016/j.bbrc.2005.06.023
hcfmusp.relation.referenceKUWAHIRA I, 1993, J APPL PHYSIOL, V74, P211
hcfmusp.relation.referenceLafontan M, 2009, PROG LIPID RES, V48, P275, DOI 10.1016/j.plipres.2009.05.001
hcfmusp.relation.referenceLi JG, 2007, J APPL PHYSIOL, V102, P557, DOI 10.1152/japplphysiol.01081.2006
hcfmusp.relation.referenceLi JG, 2005, CIRC RES, V97, P698, DOI 10.1161/01.RES.000018379.60089.a9
hcfmusp.relation.referenceLi JG, 2007, PHYSIOL GENOMICS, V31, P273, DOI 10.1152/physiolgenomics.00082.2007
hcfmusp.relation.referenceLiu LP, 2006, MOL CELL, V21, P521, DOI 10.1016/j.molcel.2006.01.010
hcfmusp.relation.referenceLivak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
hcfmusp.relation.referenceLOUHIJA A, 1969, EXPERIENTIA, V25, P248, DOI 10.1007/BF02034371
hcfmusp.relation.referenceMCELROY WT, 1961, J APPL PHYSIOL, V16, P760
hcfmusp.relation.referenceMCGARRY JD, 1973, J BIOL CHEM, V248, P270
hcfmusp.relation.referenceMotojima K, 1998, J BIOL CHEM, V273, P16710, DOI 10.1074/jbc.273.27.16710
hcfmusp.relation.referenceMuratsubaki H., 2003, Archives of Physiology and Biochemistry, V111, P449, DOI 10.3109/13813450312331342319
hcfmusp.relation.referenceNewman AB, 2001, AM J EPIDEMIOL, V154, P50, DOI 10.1093/aje/154.1.50
hcfmusp.relation.referenceNILSSONEHLE P, 1976, J LIPID RES, V17, P536
hcfmusp.relation.referenceOLIVECRONA G, 1995, CURR OPIN LIPIDOL, V6, P291, DOI 10.1097/00041433-199510000-00009
hcfmusp.relation.referencePhillips CL, 2011, AM J RESP CRIT CARE, V184, P355, DOI 10.1164/rccm.201102-0316OC
hcfmusp.relation.referenceRanganathan G, 2002, J BIOL CHEM, V277, P43281, DOI 10.1074/jbc.M202560200
hcfmusp.relation.referenceRicart-Jane D, 2005, J APPL PHYSIOL, V99, P1343, DOI 10.1152/japplphysiol.00971.2004
hcfmusp.relation.referenceRICHES AC, 1973, J PHYSIOL-LONDON, V228, P279
hcfmusp.relation.referenceRoberts AC, 1996, J APPL PHYSIOL, V81, P1762
hcfmusp.relation.referenceROSELL S, 1966, ACTA PHYSIOL SCAND, V67, P343, DOI 10.1111/j.1748-1716.1966.tb03320.x
hcfmusp.relation.referenceSchmittgen TD, 2008, NAT PROTOC, V3, P1101, DOI 10.1038/nprot.2008.73
hcfmusp.relation.referenceSCHNAKEN.DD, 1972, EXPERIENTIA, V28, P1172, DOI 10.1007/BF01946150
hcfmusp.relation.referenceSchoonjans K, 1996, EMBO J, V15, P5336
hcfmusp.relation.referenceSchweiger M, 2006, J BIOL CHEM, V281, P40236, DOI 10.1074/jbc.M608048200
hcfmusp.relation.referenceSharma SK, 2011, NEW ENGL J MED, V365, P2277, DOI 10.1056/NEJMoa1103944
hcfmusp.relation.referenceSTREJA DA, 1977, METABOLISM, V26, P505, DOI 10.1016/0026-0495(77)90094-4
hcfmusp.relation.referenceTansey JT, 2004, IUBMB LIFE, V56, P379, DOI 10.1080/15216540400009968
hcfmusp.relation.referenceUsui S, 2002, J LIPID RES, V43, P805
hcfmusp.relation.referenceWang B, 2008, J ENDOCRINOL, V198, P127, DOI 10.1677/JOE-08-0156
hcfmusp.relation.referenceWang SP, 2008, MOL GENET METAB, V95, P117, DOI 10.1016/j.ymgme.2008.06.012
hcfmusp.relation.referenceWay JM, 2001, ENDOCRINOLOGY, V142, P1269, DOI 10.1210/en.142.3.1269
hcfmusp.relation.referenceWeinstein MM, 2008, J BIOL CHEM, V283, P34511, DOI 10.1074/jbc.M806067200
hcfmusp.relation.referenceWhite UA, 2010, MOL CELL ENDOCRINOL, V318, P10, DOI 10.1016/j.mce.2009.08.023
hcfmusp.relation.referenceYoshida K, 2002, J LIPID RES, V43, P1770, DOI 10.1194/jlr.C200010-JLR200
hcfmusp.relation.referenceYOUNG PM, 1989, J APPL PHYSIOL, V66, P1430
hcfmusp.relation.referenceYun Z, 2002, DEV CELL, V2, P331, DOI 10.1016/S1534-5807(02)00131-4
hcfmusp.remissive.sponsorshipNIH
hcfmusp.scopus.lastupdate2024-05-10
relation.isAuthorOfPublication45056c2e-919d-4fc1-97d3-356e54fe3385
relation.isAuthorOfPublication.latestForDiscovery45056c2e-919d-4fc1-97d3-356e54fe3385
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
art_DRAGER_Acute_hypoxia_induces_hypertriglyceridemia_by_decreasing_plasma_triglyceride_2012.PDF
Tamanho:
2 MB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/09