A new systematization of histological analysis for the diagnosis of Hirschsprung's disease

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSERAFINI, Suellen
dc.contributor.authorSANTOS, Maria Merces
dc.contributor.authorTANNURI, Ana Cristina Aoun
dc.contributor.authorLORETO, Celso Di
dc.contributor.authorGONCALVES, Josiane de Oliveira
dc.contributor.authorTANNURI, Uenis
dc.date.accessioned2023-06-21T14:14:34Z
dc.date.available2023-06-21T14:14:34Z
dc.date.issued2023
dc.description.abstractBackground: Hirschsprung's Disease (HD) is characterized by intestinal sub-occlusion and the absence of enteric ganglion cells. A rectal biopsy examination is performed to confirm the diagnosis. In a recent study, we demon-strated that the analysis of 60 sections of rectal mucosa and submucosa stained by H&E may ensure a 90% diag-nostic accuracy. Although the need to analyze so many sections makes the process of reading the slides more time-consuming, this encouraged us to study their distribution in the healthy rectal submucosa, to simplify the diagnosis.Objectives: To develop a method that facilitates HD diagnosis by studying the distribution of ganglion cells in the submucosal plexus. Methods: Using the calretinin technique, we studied the distribution of plexuses in 60 fragments of rectal submu-cosa from 19 cadavers. After the study, the reading method created was used for diagnosis in 47 cases of suspected HD, using H&E staining. The accuracy was verified by comparing the results obtained with H&E to those obtained with the acetylcholinesterase technique, the golden standard in our laboratory.Results: The study of submucosal plexus distribution showed that just by examining the submucosal region every 20 mu m, approximately, it is possible to locate a ganglionic plexus, and we have already been able to diagnose HD with 93% accuracy. Conclusion: The study of ganglion cell distribution enabled the creation of a simplified method for reading the slides. The method applied achieved good accuracy and it can be used as an alternative method in HD diagnosis.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.indexScielo
dc.description.sponsorshipFAPESP [2019/10339-1]
dc.identifier.citationCLINICS, v.78, article ID 100198, 6p, 2023
dc.identifier.doi10.1016/j.clinsp.2023.100198
dc.identifier.eissn1980-5322
dc.identifier.issn1807-5932
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/53992
dc.language.isoeng
dc.publisherELSEVIER ESPANAeng
dc.relation.ispartofClinics
dc.rightsopenAccesseng
dc.rights.holderCopyright ELSEVIER ESPANAeng
dc.subjectHirschsprung 's Diseaseeng
dc.subjectRectal biopsyeng
dc.subjectGanglion cellseng
dc.subjectSubmucosaeng
dc.subject.otherrectal suction biopsyeng
dc.subject.othercalretinineng
dc.subject.othermegacoloneng
dc.subject.wosMedicine, General & Internaleng
dc.titleA new systematization of histological analysis for the diagnosis of Hirschsprung's diseaseeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcSUELLEN SERAFINI
hcfmusp.contributor.author-fmusphcMARIA DAS MERCES DOS SANTOS
hcfmusp.contributor.author-fmusphcANA CRISTINA AOUN TANNURI
hcfmusp.contributor.author-fmusphcCELSO DI LORETO
hcfmusp.contributor.author-fmusphcJOSIANE DE OLIVEIRA GONCALVES
hcfmusp.contributor.author-fmusphcUENIS TANNURI
hcfmusp.description.articlenumber100198
hcfmusp.description.volume78
hcfmusp.origemWOS
hcfmusp.origem.pubmed37075514
hcfmusp.origem.scieloSCIELO:S1807-59322023000100235
hcfmusp.origem.scopus2-s2.0-85152516879
hcfmusp.origem.wosWOS:000987046700001
hcfmusp.publisher.cityMADRIDeng
hcfmusp.publisher.countrySPAINeng
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