Genetic diversity of hepatitis B virus quasispecies in different biological compartments reveals distinct genotypes

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLAGO, Barbara Vieira do
dc.contributor.authorBEZERRA, Cristianne Sousa
dc.contributor.authorMOREIRA, Daniel Andrade
dc.contributor.authorPARENTE, Thiago Estevam
dc.contributor.authorPORTILHO, Moyra Machado
dc.contributor.authorPESSOA, Rodrigo
dc.contributor.authorSANABANI, Sabri Saeed
dc.contributor.authorVILLAR, Livia Melo
dc.date.accessioned2023-12-15T19:00:53Z
dc.date.available2023-12-15T19:00:53Z
dc.date.issued2023
dc.description.abstractThe selection pressure imposed by the host immune system impacts hepatitis B virus (HBV) quasispecies variability. This study evaluates HBV genetic diversity in different biological fluids. Twenty paired serum, oral fluid, and DBS samples from chronic HBV carriers were analyzed using both Sanger and next generation sequencing (NGS). The mean HBV viral load in serum was 5.19 +/- 4.3 log IU/mL (median 5.29, IQR 3.01-7.93). Genotype distribution was: HBV/A1 55% (11/20), A2 15% (3/20), D3 10% (2/20), F2 15% (3/20), and F4 5% (1/20). Genotype agreement between serum and oral fluid was 100% (genetic distances 0.0-0.006), while that between serum and DBS was 80% (genetic distances 0.0-0.115). Two individuals presented discordant genotypes in serum and DBS. Minor population analysis revealed a mixed population. All samples displayed mutations in polymerase and/or surface genes. Major population analysis of the polymerase pointed to positions H122 and M129 as the most polymorphic (>= 75% variability), followed by V163 (55%) and I253 (50%). Neither Sanger nor NGS detected any antiviral primary resistance mutations in the major populations. Minor population analysis, however, demonstrated the rtM204I resistance mutation in all individuals, ranging from 2.8 to 7.5% in serum, 2.5 to 6.3% in oral fluid, and 3.6 to 7.2% in DBS. This study demonstrated that different fluids can be used to assess HBV diversity, nonetheless, genotypic differences according to biological compartments can be observed.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.sponsorshipOswaldo Cruz IOC/Fiocruz/Ministry of Health, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico of Brazil-CNPq
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior of Brasil-CAPES [001]
dc.identifier.citationSCIENTIFIC REPORTS, v.13, n.1, article ID 17023, 10p, 2023
dc.identifier.doi10.1038/s41598-023-43655-0
dc.identifier.issn2045-2322
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/57654
dc.language.isoeng
dc.publisherNATURE PORTFOLIOeng
dc.relation.ispartofScientific Reports
dc.rightsopenAccesseng
dc.rights.holderCopyright NATURE PORTFOLIOeng
dc.subject.othermajor hydrophilic regioneng
dc.subject.otherviral replicationeng
dc.subject.otherhbveng
dc.subject.othermutationseng
dc.subject.otherbrazileng
dc.subject.otherglycosylationeng
dc.subject.otheralignmenteng
dc.subject.otherhbsageng
dc.subject.wosMultidisciplinary Scienceseng
dc.titleGenetic diversity of hepatitis B virus quasispecies in different biological compartments reveals distinct genotypeseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalLAGO, Barbara Vieira do:Fundacao Oswaldo Cruz, Lab Hepatites Virais, Inst Oswaldo Cruz, Rio De Janeiro, RJ, Brazil
hcfmusp.author.externalBEZERRA, Cristianne Sousa:Fundacao Oswaldo Cruz, Lab Hepatites Virais, Inst Oswaldo Cruz, Rio De Janeiro, RJ, Brazil; Inst Fed Educ Ciencia & Tecnol Ceara, Dept Educ, Fortaleza, CE, Brazil
hcfmusp.author.externalMOREIRA, Daniel Andrade:Fundacao Oswaldo Cruz, Lab Genomica Aplicada & Bioinovacoes, Inst Oswaldo Cruz, Rio De Janeiro, Brazil
hcfmusp.author.externalPARENTE, Thiago Estevam:Fundacao Oswaldo Cruz, Lab Genomica Aplicada & Bioinovacoes, Inst Oswaldo Cruz, Rio De Janeiro, Brazil
hcfmusp.author.externalPORTILHO, Moyra Machado:Fundacao Oswaldo Cruz, Inst Goncalo Moniz, Salvador, BA, Brazil
hcfmusp.author.externalPESSOA, Rodrigo:Fed Univ Sao Paulo UNIFESP, Dept Med, Postgrad Program Translat Med, Sao Paulo, Brazil
hcfmusp.author.externalVILLAR, Livia Melo:Fundacao Oswaldo Cruz, Lab Hepatites Virais, Inst Oswaldo Cruz, Rio De Janeiro, RJ, Brazil
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcSABRI SAEED MOHAMMED ALSANABANI
hcfmusp.description.articlenumber17023
hcfmusp.description.issue1
hcfmusp.description.volume13
hcfmusp.origemWOS
hcfmusp.origem.pubmed37813888
hcfmusp.origem.scopus2-s2.0-85173329184
hcfmusp.origem.wosWOS:001099954200069
hcfmusp.publisher.cityBERLINeng
hcfmusp.publisher.countryGERMANYeng
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