Calorie restriction increases cerebral mitochondrial respiratory capacity in a NO center dot-mediated mechanism: Impact on neuronal survival

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCERQUEIRA, Fernanda M.
dc.contributor.authorCUNHA, Fernanda M.
dc.contributor.authorLAURINDO, Francisco R. M.
dc.contributor.authorKOWALTOWSKI, Alicia J.
dc.date.accessioned2013-07-30T17:52:39Z
dc.date.available2013-07-30T17:52:39Z
dc.date.issued2012
dc.description.abstractCalorie restriction (CR) enhances animal life span and prevents age-related diseases, including neurological decline. Recent evidence suggests that a mechanism involved in CR-induced life-span extension is NO-stimulated mitochondrial biogenesis. We examine here the effects of CR on brain mitochondrial content. CR increased eNOS and nNOS and the content of mitochondria] proteins (cytochrome c oxidase, citrate synthase, and mitofusin) in the brain. Furthermore, we established an in vitro system to study the neurological effects of CR using serum extracted from animals on this diet. In cultured neurons, CR serum enhanced nNOS expression and increased levels of nitrite (a NO product). CR serum also enhanced the levels of cytochrome c oxidase and increased citrate synthase activity and respiratory rates in neurons. CR serum effects were inhibited by L-NAME and mimicked by the NO donor SNAP. Furthermore, both CR sera and SNAP were capable of improving neuronal survival. Overall, our results indicate that CR increases mitochondrial biogenesis in a NO-mediated manner, resulting in enhanced reserve respiratory capacity and improved survival in neurons.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia de Processos Redox em Biomedicina
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipJohn Simon Guggenheim Memorial Foundation
dc.identifier.citationFREE RADICAL BIOLOGY AND MEDICINE, v.52, n.7, p.1236-1241, 2012
dc.identifier.doi10.1016/j.freeradbiomed.2012.01.011
dc.identifier.issn0891-5849
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/1517
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofFree Radical Biology and Medicine
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER SCIENCE INC
dc.subjectCaloric restriction
dc.subjectnitric oxide synthase
dc.subjectmitochondrial biogenesis
dc.subjectaging
dc.subject.otherendogenous nitric-oxide
dc.subject.otherskeletal-muscle
dc.subject.otheroxidative stress
dc.subject.othercortical-neurons
dc.subject.others-nitrosylation
dc.subject.othercomplex-i
dc.subject.otherbiogenesis
dc.subject.otherglutamate
dc.subject.otherbrain
dc.subject.othermice
dc.subject.wosBiochemistry & Molecular Biology
dc.subject.wosEndocrinology & Metabolism
dc.titleCalorie restriction increases cerebral mitochondrial respiratory capacity in a NO center dot-mediated mechanism: Impact on neuronal survival
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalCERQUEIRA, Fernanda M.:Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalCUNHA, Fernanda M.:Univ Sao Paulo, Escola Artes Ciencias & Humanindades, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalKOWALTOWSKI, Alicia J.:Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508900 Sao Paulo, Brazil
hcfmusp.citation.scopus51
hcfmusp.contributor.author-fmusphcFRANCISCO RAFAEL MARTINS LAURINDO
hcfmusp.description.beginpage1236
hcfmusp.description.endpage1241
hcfmusp.description.issue7
hcfmusp.description.volume52
hcfmusp.lim.ref2012
hcfmusp.origemWOS
hcfmusp.origem.pubmed22310960
hcfmusp.origem.scopus2-s2.0-84857921494
hcfmusp.origem.wosWOS:000301628200011
hcfmusp.publisher.cityNEW YORK
hcfmusp.publisher.countryUSA
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hcfmusp.remissive.sponsorshipCAPES
hcfmusp.remissive.sponsorshipCNPq
hcfmusp.remissive.sponsorshipFAPESP
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