Organic and Inorganic Fractions of Diesel Exhaust Particles Produce Changes in Mucin Profile of Mouse Trachea Explants

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSERIANI, Robson
dc.contributor.authorJUNQUEIRA, Mara S.
dc.contributor.authorTOLEDO, Alessandra C.
dc.contributor.authorCORREA, Aristides T.
dc.contributor.authorSILVA, Luiz F. F.
dc.contributor.authorMARTINS, Milton A.
dc.contributor.authorSALDIVA, Paulo H. N.
dc.contributor.authorMAUAD, Thais
dc.contributor.authorMACCHIONE, Mariangela
dc.date.accessioned2015-07-01T20:17:43Z
dc.date.available2015-07-01T20:17:43Z
dc.date.issued2015
dc.description.abstractDiesel exhaust particles (DEP) contain organic and inorganic elements that produce damage to the respiratory epithelium. The aim of this study was to determine the mucus profile of tracheal explants exposed to either crude diesel exhaust particles (DEP) or DEP treated with nitric acid (DEP/NA), with hexane (DEP/HEX), or with methanol (DEP/MET) at concentrations of 50 and 100 mu g/ml for 30 and 60 min. Tracheal explants were subjected to morphometric analyses to study acidic (AB+), neutral (PAS+), and mixed (AB+/PAS+) mucus production and vacuolization (V). Incubation with 50 mu g/ml crude DEP resulted in a rise in acid mucus production, an increase in vacuolization at 30 min, and reduction in neutral mucus at 30 and 60 min. Tracheas exposed to DEP/MET at 50 mu g/ml for 30 or 60 min resulted in a significant decrease in neutral mucus production and an elevation in acid mucus production. DEP/HEX increased vacuolization at both 50 and 100 mu g/ml at 30 and 60 min of exposure. Treatment with 50 mu g/ml for 30 or 60 min significantly elevated mixed mucus levels. These results suggest that DEP appear to be more toxic when administered in combination with HEX or MET. DEP/MET modified the mucus profile of the epithelium, while DEP/HEX altered mucus extrusion, and these responses might be due to bioavailability of individual elements in DEP fractions.
dc.description.indexMEDLINE
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq [Processo: 484189/2007-7]
dc.description.sponsorshipHospital das Clinicas, Faculdade de Medicina da Universidade de Sao Paulo, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP [2011/50334-7]
dc.identifier.citationJOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.78, n.4, p.215-225, 2015
dc.identifier.doi10.1080/15287394.2014.947456
dc.identifier.eissn1087-2620
dc.identifier.issn1528-7394
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/9250
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS INC
dc.relation.ispartofJournal of Toxicology and Environmental Health-Part A-current Issues
dc.rightsrestrictedAccess
dc.rights.holderCopyright TAYLOR & FRANCIS INC
dc.subject.otherallergic airway inflammation
dc.subject.otherbronchial epithelial-cells
dc.subject.otherpulmonary inflammation
dc.subject.otherinhalation exposure
dc.subject.otherpollution particles
dc.subject.otheroxidative stress
dc.subject.othersulfur-dioxide
dc.subject.otherterm exposure
dc.subject.othernasal-mucosa
dc.subject.othermice
dc.subject.wosEnvironmental Sciences
dc.subject.wosPublic, Environmental & Occupational Health
dc.subject.wosToxicology
dc.titleOrganic and Inorganic Fractions of Diesel Exhaust Particles Produce Changes in Mucin Profile of Mouse Trachea Explants
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.citation.scopus9
hcfmusp.contributor.author-fmusphcROBSON SERIANI
hcfmusp.contributor.author-fmusphcMARA DE SOUZA JUNQUEIRA
hcfmusp.contributor.author-fmusphcALESSANDRA CHOQUETA DE TOLEDO ARRUDA
hcfmusp.contributor.author-fmusphcARISTIDES TADEU CORREIA
hcfmusp.contributor.author-fmusphcVITOR BRITO DA SILVA
hcfmusp.contributor.author-fmusphcMILTON DE ARRUDA MARTINS
hcfmusp.contributor.author-fmusphcPAULO HILARIO NASCIMENTO SALDIVA
hcfmusp.contributor.author-fmusphcTHAIS MAUAD
hcfmusp.contributor.author-fmusphcMARIANGELA MACCHIONE
hcfmusp.description.beginpage215
hcfmusp.description.endpage225
hcfmusp.description.issue4
hcfmusp.description.volume78
hcfmusp.origemWOS
hcfmusp.origem.pubmed25674825
hcfmusp.origem.scopus2-s2.0-84923328610
hcfmusp.origem.wosWOS:000349330600001
hcfmusp.publisher.cityPHILADELPHIA
hcfmusp.publisher.countryUSA
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