Evaluation of a High Throughput Method for the Detection of Mutations Associated with Thrombosis and Hereditary Hemochromatosis in Brazilian Blood Donors
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4
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article
Data de publicação
2015
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PUBLIC LIBRARY SCIENCE
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PLOS ONE, v.10, n.5, article ID UNSP e0125460, 9p, 2015
Resumo
Background The aim of this study was to evaluate the OpenArray platform for genetic testing of blood donors and to assess the genotype frequencies of nucleotide-polymorphisms (SNPs) associated with venous thrombosis (G1691A and G20210A), hyperhomocysteinemia (C677T, A1298C), and hereditary hemochromatosis (C282Y, H63D and S65C) in blood donors from Sao Paulo, Brazil. Methods We examined 400 blood donor samples collected from October to November 2011. The SNPs were detected using OpenArray technology. The blood samples were also examined using a real-time PCR-FRET system to compare the results and determine the accuracy of the OpenArray method. Results We observed 100% agreement in all assays tested, except HFE C282Y, which showed 99.75% agreement. The HFE C282Y assay was further confirmed through direct sequencing, and the results showed that OpenArray analysis was accurate. The calculated frequencies of each SNP were FV G1691A 98.8% (G/G), 1.2% (G/A); FII G2021A 99.5% (G/G), 0.5% (G/A); MTHFR C677T 45.5%(C/C), 44.8% (C/T), 9.8% (T/T); MTHFR A1298C 60.3% (A/A), 33.6% (A/C), 6.1% (C/C); HFE C282Y 96%(G/G), 4%(G/A), HFE H63D 78.1%(C/C), 20.3% (C/G), 1.6% (G/G); and HFE S65C 98.1%(A/A), 1.9% (A/T). Conclusion Taken together, these results describe the frequencies of SNPs associated with diseases and are important to enhance our current knowledge of the genetic profiles of Brazilian blood donors, although a larger study is needed for a more accurate determination of the frequency of the alleles. Furthermore, the OpenArray platform showed a high concordance rate with standard FRET RT-PCR.
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Referências
- Alessio ACM, 2004, AM J MED GENET A, V128A, P256, DOI 10.1002/ajmg.a.30108
- Alexander J, 2009, GENET MED, V11, P307, DOI [10.1097/GIM.0b013e31819d30f2, 10.1097/GIM.0b013e31819d3012]
- Anstee DJ, 2009, BLOOD, V114, P248, DOI 10.1182/blood-2008-11-146860
- Aznar J, 2013, AM J OBSTET GYNECOL, V209, P156, DOI 10.1016/j.ajog.2013.02.036
- Bauduer F, 2005, MOL GENET METAB, V86, P91, DOI 10.1016/j.ymgme.2005.04.002
- Bernard PS, 1998, AM J PATHOL, V153, P1055, DOI 10.1016/S0002-9440(10)65650-7
- BERTINA RM, 1994, NATURE, V369, P64, DOI 10.1038/369064a0
- Beutler E, 2002, LANCET, V359, P211, DOI 10.1016/S0140-6736(02)07447-0
- Bezemer ID, 2008, JAMA-J AM MED ASSOC, V299, P1306, DOI 10.1001/jama.299.11.1306
- Cooper DN, 2013, HUM GENET, V132, P1077, DOI 10.1007/s00439-013-1331-2
- Dalmaz CA, 2006, BLOOD CELL MOL DIS, V37, P107, DOI 10.1016/j.bcmd.2006.07.005
- Davis CF, 2010, ANN HEMATOL, V89, P375, DOI 10.1007/s00277-009-0839-y
- Sabino AD, 2007, J THROMB THROMBOLYS, V24, P261, DOI 10.1007/s11239-007-0024-x
- Delluc A, 2010, THROMB HAEMOSTASIS, V103, P1161, DOI 10.1160/TH09-07-0430
- De Stefano V, 2013, THROMB HAEMOSTASIS, V110, P697, DOI 10.1160/TH13-01-0011
- De Stefano V, 1999, NEW ENGL J MED, V341, P801, DOI 10.1056/NEJM199909093411104
- Dusse LMS, 2007, EUR J OBSTET GYN R B, V134, P20, DOI 10.1016/j.ejogrb.2006.09.006
- Eldibany MM, 2007, ARCH PATHOL LAB MED, V131, P872
- Endler G, 2003, CLIN CHIM ACTA, V330, P31, DOI 10.1016/S0009-8981(03)00022-6
- Filho Isaac Lima da Silva, 2011, Arq Neuropsiquiatr, V69, P431
- Goette L, 2009, TRANSFUSION, V49, P524, DOI 10.1111/j.1537-2995.2008.02007.x
- Jacques PF, 1996, CIRCULATION, V93, P7
- Kvasnicka Jan, 2012, Cas Lek Cesk, V151, P76
- Lee DH, 1996, CAN MED ASSOC J, V155, P285
- Leitman SF, 2013, HEMATOL-AM SOC HEMAT, P645
- Lenhard MJ, 2013, TRANSFUSION, V53, P2776, DOI 10.1111/trf.12135
- Lima MBPLV, 2009, GENET MOL BIOL, V32, P264, DOI 10.1590/S1415-47572009000200010
- Madjunkova S, 2012, GENET TEST MOL BIOMA, V16, P259, DOI 10.1089/gtmb.2011.0173
- Mira Y, 2000, CLIN APPL THROMB-HEM, V6, P162, DOI 10.1177/107602960000600308
- Morrison T, 2006, NUCLEIC ACIDS RES, V34, DOI 10.1093/nar/gkl639
- Pena SDJ, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0017063
- Pennings G, 2005, J MED ETHICS, V31, P69, DOI 10.1136/jme.2002.001271
- Poort SR, 1996, BLOOD, V88, P3698
- Ramos Catarina Paula da Silva, 2008, J. Bras. Patol. Med. Lab., V44, P79, DOI 10.1590/S1676-24442008000200003
- Ramos Catarina P. S., 2006, Rev. Bras. Hematol. Hemoter., V28, P131, DOI 10.1590/S1516-84842006000200013
- Santos PCJL, 2012, INT J MOL SCI, V13, P1497, DOI 10.3390/ijms13021497
- Santos PCJL, 2010, BLOOD CELL MOL DIS, V45, P302, DOI 10.1016/j.bcmd.2010.08.008
- Santos PCJL, 2010, BRAZ J MED BIOL RES, V43, P107, DOI 10.1590/S0100-879X2009007500031
- Santos RV, 2009, CURR ANTHROPOL, V50, P787, DOI 10.1086/644532
- Sazci A, 2006, BRAIN RES BULL, V71, P45, DOI 10.1016/j.brainresbull.2006.07.014
- Sottilotta G, 2009, CLIN APPL THROMB-HEM, V15, P356, DOI 10.1177/1076029607310218
- Spinola C, 2011, ANN HEMATOL, V90, P29, DOI 10.1007/s00277-010-1034-x
- Stur E, 2012, GENET TEST MOL BIOMA, V16, P219, DOI 10.1089/gtmb.2011.0163
- Terada CT, 2009, TRANSFUSION MED, V19, P245, DOI 10.1111/j.1365-3148.2009.00944.x
- van den Bergh FAJTM, 2000, CLIN CHEM, V46, P1191
- Vares M, 2010, AM J MED GENET B, V153B, P610, DOI 10.1002/ajmg.b.31030
- Westhoff CM, 2006, CURR OPIN HEMATOL, V13, P471, DOI 10.1097/01.moh.0000245695.77758.3d
- Zappacosta B, 2014, CELL BIOCHEM FUNCT, V32, P1, DOI 10.1002/cbf.3019