CXCR3 Controls T-Cell Accumulation in Fat Inflammation

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Arquivos
art_ROCHA_CXCR3_Controls_T_Cell_Accumulation_in_Fat_Inflammation_2014.PDF (1 MB)
Citações na Scopus
28
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
LIPPINCOTT WILLIAMS & WILKINS
Autores
FOLCO, Eduardo J.
OZDEMIR, Cafer
SHEIKINE, Yuri
CHRISTEN, Thomas
SUKHOVA, Galina K.
TANG, Eva H. C.
LUSTER, Andrew D.
Citação
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, v.34, n.7, p.1374-+, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Objective-Obesity associates with increased numbers of inflammatory cells in adipose tissue (AT), including T cells, but the mechanism of T-cell recruitment remains unknown. This study tested the hypothesis that the chemokine (C-X-C motif) receptor 3 (CXCR3) participates in T-cell accumulation in AT of obese mice and thus in the regulation of local inflammation and systemic metabolism. Approach and Results-Obese wild-type mice exhibited higher mRNA expression of CXCR3 in periepididymal AT-derived stromal vascular cells compared with lean mice. We evaluated the function of CXCR3 in AT inflammation in vivo using CXCR3-deficient and wild-type control mice that consumed a high-fat diet. Periepididymal AT from obese CXCR3-deficient mice contained fewer T cells than obese controls after 8 and 16 weeks on high-fat diet, as assessed by flow cytometry. Obese CXCR3-deficient mice had greater glucose tolerance than obese controls after 8 weeks, but not after 16 weeks. CXCR3-deficient mice fed high-fat diet had reduced mRNA expression of proinflammatory mediators, such as monocyte chemoattractant protein-1 and regulated on activation, normal T cell expressed and secreted, and anti-inflammatory genes, such as Foxp3, IL-10, and arginase-1 in periepididymal AT, compared with obese controls. Conclusions-These results demonstrate that CXCR3 contributes to T-cell accumulation in periepididymal AT of obese mice. Our results also suggest that CXCR3 regulates the accumulation of distinct subsets of T cells and that the ratio between these functional subsets across time likely modulates local inflammation and systemic metabolism.
Palavras-chave
adipose tissue, CXCR3, receptors, inflammation, lymphocytes, macrophages, obesity
URI
https://observatorio.fm.usp.br/handle/OPI/7436
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCP
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - ODS/03