Acute, subacute toxicity and mutagenic effects of anacardic acids from cashew (Anacardium occidentale Linn.) in mice

Imagem de Miniatura
Arquivos
art_CARVALHO_Acute_subacute_toxicity_and_mutagenic_effects_of_anacardic_2011.PDF (235.97 KB)
Citações na Scopus
52
Tipo de produção
article
Data de publicação
2011
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER IRELAND LTD
Autores
BORELLI, Primavera
FOCK, Ricardo Ambrosio
TREVISAN, Maria Teresa Salles
Citação
JOURNAL OF ETHNOPHARMACOLOGY, v.135, n.3, p.730-736, 2011
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Aim of the study: Anacardium occidentale Linn. (cashew) is a Brazilian plant that is usually consumed in natura and is used in folk medicine. Anacardic acids (AAs) in the cashew nut shell liquid are biologically active as gastroprotectors, inhibitors of the activity of various deleterious enzymes, antitumor agents and antioxidants. Yet, there are no reports of toxicity testing to guarantee their use in vivo models. Materials and methods: We evaluated AAs biosafety by measuring the acute, subacute and mutagenic effects of AAs administration in BALB/c mice. In acute tests, BALB/c mice received a single oral dose of 2000 mg/kg, whereas animals in subacute tests received 300, 600 and 1000 mg/kg for 30 days. Hematological, biochemical and histological analyses were performed in all animals. Mutagenicity was measured with the acute micronucleus test 24 h after oral administration of 250 mg/kg AAs. Results: Our results showed that the AAs acute minimum lethal dose in BALB/c mice is higher than 2000 mg/kg since this concentration did not produce any symptoms. In subacute tests, females which received the highest doses (600 or 1000 mg/kg) were more susceptible, which was seen by slightly decreased hematocrit and hemoglobin levels coupled with a moderate increase in urea. Anacardic acids did not produce any mutagenic effects. Conclusions: The data indicate that doses less than 300 mg/kg did not produce biochemical and hematological alterations in BALB/c mice. Additional studies must be conducted to investigate the pharmacological potential of this natural substance in order to ensure their safe use in vivo.
Palavras-chave
Anacardic acids, Anacardium occidentale, Acute toxicity, Subacute toxicity, Mutagenicity
URI
https://observatorio.fm.usp.br/handle/OPI/23495
Referências
  1. ALMEIDA AS, 2008, BRAZILIAN J PATHOLOG, V44, P429
  2. Masuoka N, 2004, BBA-MOL BASIS DIS, V1688, P245, DOI 10.1016/j.bbadis.2003.12.010
  3. Dekker FJ, 2009, DRUG DISCOV TODAY, V14, P942, DOI 10.1016/j.drudis.2009.06.008
  4. Paramashivappa R, 2001, J AGR FOOD CHEM, V49, P2548, DOI 10.1021/jf001222j
  5. Kubo I, 2006, FOOD CHEM, V99, P555, DOI 10.1016/j.foodchem.2005.08.023
  6. Kubo J, 1999, J AGR FOOD CHEM, V47, P533, DOI 10.1021/jf9808980
  7. Paramashivappa R, 2003, BIOORG MED CHEM LETT, V13, P657, DOI 10.1016/S0960-894X(02)01006-5
  8. Tsai PP, 2002, LAB ANIM-UK, V36, P411, DOI 10.1258/002367702320389071
  9. Konan NA, 2007, J ETHNOPHARMACOL, V110, P30, DOI 10.1016/j.jep.2006.08.033
  10. Kubo I, 2003, J AGR FOOD CHEM, V51, P7624, DOI 10.1021/jf034674f
  11. Olajide OA, 2004, J ETHNOPHARMACOL, V95, P139, DOI 10.1016/j.jep.2004.06.033
  12. KUBO I, 1994, J NAT PROD, V57, P545, DOI 10.1021/np50106a021
  13. Nemzek JA, 2001, INFLAMM RES, V50, P523, DOI 10.1007/PL00000229
  14. KUBO I, 1993, J AGR FOOD CHEM, V41, P1012, DOI 10.1021/jf00030a035
  15. MOTA MLR, 1985, J ETHNOPHARMACOL, V13, P289
  16. Konan NA, 2007, J ETHNOPHARMACOL, V112, P237, DOI 10.1016/j.jep.2007.03.003
  17. Sung B, 2008, BLOOD, V111, P4880, DOI 10.1182/blood-2007-10-117994
  18. MACGREGOR JT, 1987, MUTAT RES, V189, P103, DOI 10.1016/0165-1218(87)90016-4
  19. Morais TC, 2010, CHEM-BIOL INTERACT, V183, P264, DOI 10.1016/j.cbi.2009.10.008
  20. Cavalcante AAM, 2003, ENVIRON MOL MUTAGEN, V41, P360, DOI 10.1002/em.10158
  21. Muroi H, 1996, J APPL BACTERIOL, V80, P387, DOI 10.1111/j.1365-2672.1996.tb03233.x
  22. Ramaiah SK, 2007, FOOD CHEM TOXICOL, V45, P1551, DOI 10.1016/j.fct.2007.007
  23. Rodrigues FHA, 2006, J BRAZIL CHEM SOC, V17, P265, DOI 10.1590/S0103-50532006000200008
  24. Sun YL, 2006, FEBS LETT, V580, P4353, DOI 10.1016/j.febslet.2006.06.092
  25. Acevedo HR, 2006, MUTAT RES-GEN TOX EN, V609, P43, DOI 10.1016/j.mrgentox.2006.06.002
  26. Barcelos GRM, 2007, J ETHNOPHARMACOL, V114, P268, DOI 10.1016/j.jep.2007.08.006
  27. Barcelos GRM, 2007, TOXICOL IN VITRO, V21, P1468, DOI 10.1016/j.tiv.2007.06.006
  28. Barrios M, 2009, THROMB RES, V124, P338, DOI 10.1016/j.thromres.2008.11.001
  29. EPA, 2000, 712C00366 EPA
  30. *EPA, 2002, 712C02190 EPA
  31. GRAZZINI R, 1991, BIOCHEM BIOPH RES CO, V176, P775, DOI 10.1016/S0006-291X(05)80252-9
  32. Green IR, 2008, EUR J MED CHEM, V43, P1315, DOI 10.1016/j.ejmech.2007.08.012
  33. Green IR, 2007, BIOORGAN MED CHEM, V15, P6236, DOI 10.1016/j.bmc.2007.06.022
  34. Ha TJ, 2005, J AGR FOOD CHEM, V53, P4350, DOI 10.1021/jf048184e
  35. Mazzaccara C, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003772
  36. MELO AFM, 2004, REV BRASILEIRA CIENC, V8, P217
  37. Narasimhan B, 2008, J FOOD PROCESS PRES, V32, P600, DOI 10.1111/j.1745-4549.2008.00201.x
  38. Satyanarayana PSV, 2001, METHOD FIND EXP CLIN, V23, P175, DOI 10.1358/mf.2001.23.4.634641
  39. SCHMID W., 1976, PRINCIPLES METHODS T, V4, P31
  40. SHOBHA SV, 1994, J NAT PRODUCTS, V57, P1755, DOI 10.1021/np50114a025
  41. TREVISAN MTS, 2006, FOOD CHEM TOXICOL, V44, P88
  42. *U SAO PAUL SAO PA, 2000, PAR BIOL PRINC AN EX

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/05