Growth Responses Following a Single Intra-Muscular hGH Plasmid Administration Compared to Daily Injections of hGH in Dwarf Mice

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorHIGUTI, Eliza
dc.contributor.authorCECCHI, Claudia R.
dc.contributor.authorOLIVEIRA, Nelio A. J.
dc.contributor.authorVIEIRA, Daniel P.
dc.contributor.authorJENSEN, Thomas G.
dc.contributor.authorJORGE, Alexander A. L.
dc.contributor.authorBARTOLINI, Paolo
dc.contributor.authorPERONI, Cibele N.
dc.date.accessioned2013-07-30T14:41:59Z
dc.date.available2013-07-30T14:41:59Z
dc.date.issued2012
dc.description.abstractIn previous work, sustained levels of circulating human growth hormone (hGH) and a highly significant weight increase were observed after electrotransfer of naked plasmid DNA (hGH-DNA) into the muscle of immunodeficient dwarf mice (lit/scid). In the present study, the efficacy of this in vivo gene therapy strategy is compared to daily injections (5 mu g/twice a day) of recombinant hGH (r-hGH) protein, as assessed on the basis of several growth parameters. The slopes of the two growth curves were found to be similar (P>0.05): 0.095 g/mouse/d for protein and 0.094 g/mouse/d for DNA injection. In contrast, the weight increases averaged 35.5% (P<0.001) and 23.1% (P<0.01) for protein and DNA administration, respectively, a difference possibly related to the electroporation methodology. The nose-to-tail linear growth increases were 15% and 9.6% for the protein and DNA treatments, respectively, but mouse insulin-like growth factor I (mIGF-I) showed a greater increase over the control with DNA (5- to 7-fold) than with protein (3- to 4-fold) administration. The weight increases of several organs and tissues (kidneys, spleen, liver, heart, quadriceps and gastrocnemius muscles) were 1.3- to 4.6-fold greater for protein than for DNA administration, which gave a generally more proportional growth. Glucose levels were apparently unaffected, suggesting the absence of effects on glucose tolerance. A gene transfer strategy based on a single hGH-DNA administration thus appears to be comparable to repeated hormone injections for promoting growth and may represent a feasible alternative for the treatment of growth hormone deficiency.
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP, Sao Paulo, Brazil [2010/51116-0, 2010/51848-1, 2011/21708-6]
dc.description.sponsorshipNational Research Council (CNPq), Brasilia, Brazil [143639/2008-0, PQ 300473/2009-5]
dc.identifier.citationCURRENT GENE THERAPY, v.12, n.6, p.437-443, 2012
dc.identifier.doi10.2174/156652312803519797
dc.identifier.issn1566-5232
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/514
dc.language.isoeng
dc.publisherBENTHAM SCIENCE PUBL LTD
dc.relation.ispartofCurrent Gene Therapy
dc.rightsrestrictedAccess
dc.rights.holderCopyright BENTHAM SCIENCE PUBL LTD
dc.subjecthuman growth hormone
dc.subjectimmunodeficient little mice
dc.subjectmouse insulin-like growth factor I
dc.subjectnaked DNA
dc.subject.otherhormone-releasing-hormone
dc.subject.otherphase-i trial
dc.subject.otherprimary human keratinocytes
dc.subject.otherlong-term growth
dc.subject.othergene-therapy
dc.subject.otherhypophysectomized mice
dc.subject.otheranimal-model
dc.subject.otherexpression
dc.subject.otherelectroporation
dc.subject.otherdeficiency
dc.subject.wosGenetics & Heredity
dc.titleGrowth Responses Following a Single Intra-Muscular hGH Plasmid Administration Compared to Daily Injections of hGH in Dwarf Mice
dc.typearticle
dc.type.categoryreview
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryDinamarca
hcfmusp.affiliation.countryisodk
hcfmusp.author.externalHIGUTI, Eliza:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.author.externalCECCHI, Claudia R.:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.author.externalOLIVEIRA, Nelio A. J.:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.author.externalVIEIRA, Daniel P.:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.author.externalJENSEN, Thomas G.:Aarhus Univ, Dept Biomed, DK-8000 Aarhus C, Denmark
hcfmusp.author.externalBARTOLINI, Paolo:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.author.externalPERONI, Cibele N.:Natl Nucl Energy Commiss IPEN CNEN, Dept Biotechnol, Sao Paulo, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcALEXANDER AUGUSTO DE LIMA JORGE
hcfmusp.description.beginpage437
hcfmusp.description.endpage443
hcfmusp.description.issue6
hcfmusp.description.volume2012
hcfmusp.lim.ref2012
hcfmusp.origemWOS
hcfmusp.origem.pubmed22974419
hcfmusp.origem.scopus2-s2.0-84870164679
hcfmusp.origem.wosWOS:000313577900001
hcfmusp.publisher.citySHARJAH
hcfmusp.publisher.countryU ARAB EMIRATES
hcfmusp.relation.referenceBaumgartner I, 2009, MOL THER, V17, P914, DOI 10.1038/mt.2009.24
hcfmusp.relation.referenceBellini MH, 2003, FASEB J, V17, P2322, DOI 10.1096/fj.03-0018fje
hcfmusp.relation.referenceBELLINI MH, 1993, ENDOCRINOLOGY, V132, P2051, DOI 10.1210/en.132.5.2051
hcfmusp.relation.referenceBodles-Brakhop AM, 2009, MOL THER, V17, P585, DOI 10.1038/mt.2009.5
hcfmusp.relation.referenceBraun S, 2008, CURR GENE THER, V8, P391, DOI 10.2174/156652308786070998
hcfmusp.relation.referenceBrown PA, 2009, BMC BIOTECHNOL, V9, DOI 10.1186/1472-6750-9-4
hcfmusp.relation.referenceDagnaes-Hansen F, 2002, J MOL MED-JMM, V80, P665, DOI 10.1007/s00109-002-0371-1
hcfmusp.relation.referenceDaud AI, 2008, J CLIN ONCOL, V26, P5896, DOI 10.1200/JCO.2007.15.6794
hcfmusp.relation.referenceDeitel K, 2002, J SURG ONCOL, V81, P75, DOI 10.1002/jso.10136
hcfmusp.relation.referenceDraghia-Akli R, 2006, COMB CHEM HIGH T SCR, V9, P181, DOI 10.2174/138620706776055502
hcfmusp.relation.referenceDraghia-Akli R, 2002, MOL THER, V6, P830, DOI 10.1006/mthe.2002.0807
hcfmusp.relation.referenceDraghiaAkli R, 1997, NAT BIOTECHNOL, V15, P1285, DOI 10.1038/nbt1197-1285
hcfmusp.relation.referenceDraghia-Akli R, 2002, FASEB J, V16, P426, DOI 10.1096/fj.01-0702fje
hcfmusp.relation.referenceDraghia-Akli R, 1999, NAT BIOTECHNOL, V17, P1179, DOI 10.1038/70718
hcfmusp.relation.referenceEICHER EM, 1976, J HERED, V67, P87
hcfmusp.relation.referenceFattori E, 2005, J GENE MED, V7, P228, DOI 10.1002/jgm.652
hcfmusp.relation.referenceGazdhar A, 2006, J GENE MED, V8, P910, DOI 10.1002/jgm.911
hcfmusp.relation.referenceGothelf A, 2010, CURR GENE THER, V10, P287
hcfmusp.relation.referenceGothelf A, 2010, GENE THER, V17, P1077, DOI 10.1038/gt.2010.46
hcfmusp.relation.referenceGupta R, 2009, CIRC RES, V105, P724, DOI 10.1161/CIRCRESAHA.109.200386
hcfmusp.relation.referenceJANSSON JO, 1986, SCIENCE, V232, P511, DOI 10.1126/science.3008329
hcfmusp.relation.referenceKhamaisi M, 2007, GROWTH HORM IGF RES, V17, P279, DOI 10.1016/j.ghir.2007.01.018
hcfmusp.relation.referenceKhan AS, 2010, MOL THER, V18, P327, DOI 10.1038/mt.2009.224
hcfmusp.relation.referenceLIN SC, 1993, NATURE, V364, P208, DOI 10.1038/364208a0
hcfmusp.relation.referenceLosordo DW, 2002, CIRCULATION, V105, P2012, DOI 10.1161/01.CIR.0000015982.70785.B7
hcfmusp.relation.referenceMelman A, 2007, ISRAEL MED ASSOC J, V9, P143
hcfmusp.relation.referenceMichaluart P, 2008, CANCER GENE THER, V15, P676, DOI 10.1038/cgt.2008.35
hcfmusp.relation.referenceMolitch ME, 2011, J CLIN ENDOCR METAB, V96, P1587, DOI 10.1210/jc.2011-0179
hcfmusp.relation.referenceMorishita R, 2011, ARTERIOSCL THROM VAS, V31, P713, DOI 10.1161/ATVBAHA.110.219550
hcfmusp.relation.referenceMurakami T, 2011, CURR GENE THER, V11, P447
hcfmusp.relation.referenceOliveira JE, 1999, J CHROMATOGR A, V852, P441
hcfmusp.relation.referenceOliveira NAJ, 2010, J GENE MED, V12, P580, DOI 10.1002/jgm.1470
hcfmusp.relation.referencePeroni CN, 2005, CURR GENE THER, V5, P493, DOI 10.2174/156652305774329258
hcfmusp.relation.referencePeroni CN, 2008, J GENE MED, V10, P734, DOI 10.1002/jgm.1196
hcfmusp.relation.referencePeroni CN, 2006, MOL BIOTECHNOL, V34, P239, DOI 10.1385/MB:34:2:239
hcfmusp.relation.referencePrud'homme GJ, 2007, GENE THER, V14, P553, DOI 10.1038/sj.gt.3302907
hcfmusp.relation.referenceRatanamart J, 2006, CURR GENE THER, V6, P93, DOI 10.2174/156652306775515583
hcfmusp.relation.referenceRIBELA MTCP, 1993, J IMMUNOL METHODS, V159, P269, DOI 10.1016/0022-1759(93)90166-5
hcfmusp.relation.referenceRocha MGM, 2008, J PEDIATR ENDOCR MET, V21, P673
hcfmusp.relation.referenceSalvatori R, 2004, REV ENDOCR METAB DIS, V5, P15, DOI 10.1023/B:REMD.0000016121.58762.6d
hcfmusp.relation.referenceSchorpp M, 1996, NUCLEIC ACIDS RES, V24, P1787, DOI 10.1093/nar/24.9.1787
hcfmusp.relation.referenceSondergaard M, 2003, AM J PHYSIOL-ENDOC M, V285, pE427, DOI 10.1152/ajpendo.00573.2002
hcfmusp.relation.referenceVijayakumar A, 2010, GROWTH HORM IGF RES, V20, P1, DOI 10.1016/j.ghir.2009.09.002
hcfmusp.remissive.sponsorshipCNPq
hcfmusp.remissive.sponsorshipFAPESP
hcfmusp.scopus.lastupdate2024-05-17
relation.isAuthorOfPublication872493f0-3232-484e-a574-207e16fcf202
relation.isAuthorOfPublication.latestForDiscovery872493f0-3232-484e-a574-207e16fcf202
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
art_JORGE_Growth_Responses_Following_a_Single_Intra_Muscular_hGH_2012.PDF
Tamanho:
764.8 KB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/25