Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSANTOS, Keity Souza
dc.contributor.authorGADERMAIER, Gabriele
dc.contributor.authorVEJVAR, Eva
dc.contributor.authorARCURI, Helen Andrade
dc.contributor.authorGALVAO, Clovis Eduardo
dc.contributor.authorYANG, Ariana Campos
dc.contributor.authorRESENDE, Virginia Maria Ferreira
dc.contributor.authorMARTINS, Carlo de Oliveira
dc.contributor.authorHIMLY, Martin
dc.contributor.authorMARI, Adriano
dc.contributor.authorLISO, Marina
dc.contributor.authorPOMPONI, Debora
dc.contributor.authorBREITENEDER, Heimo
dc.contributor.authorWAGNER, Stefan
dc.contributor.authorKALIL, Jorge
dc.contributor.authorFERREIRA, Fatima
dc.contributor.authorCASTRO, Fabio Fernandes Morato
dc.date.accessioned2013-09-23T16:35:57Z
dc.date.available2013-09-23T16:35:57Z
dc.date.issued2013
dc.description.abstractScope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods and results The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome. Conclusion Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP [2008/05595-4]
dc.description.sponsorshipINCT-Instituto Nacional de Ciencia e Tecnologia
dc.description.sponsorshipThermofisher Scientific, Brazil
dc.description.sponsorshipAustrian Science Fund (FWF)
dc.description.sponsorshipChristian Doppler Research Association
dc.description.sponsorshipBiomay AG
dc.identifier.citationMOLECULAR NUTRITION & FOOD RESEARCH, v.57, n.6, Special Issue, p.1100-1109, 2013
dc.identifier.doi10.1002/mnfr.201200433
dc.identifier.issn1613-4125
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/1709
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofMolecular Nutrition & Food Research
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subjectFood allergy
dc.subjectHev b 5
dc.subjectIgE cross-reactivity
dc.subjectIntrinsically disordered protein
dc.subjectLatex-fruit syndrome
dc.subjectNew allergen
dc.subject.othernatively unfolded proteins
dc.subject.otherige-binding epitopes
dc.subject.otherfruit syndrome
dc.subject.otheranaphylactic reaction
dc.subject.otherreactivity
dc.subject.othercassava
dc.subject.otheridentification
dc.subject.otherbrasiliensis
dc.subject.othersequence
dc.subject.otherregions
dc.subject.wosFood Science & Technology
dc.titleNovel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryItália
hcfmusp.affiliation.countryÁustria
hcfmusp.affiliation.countryisoat
hcfmusp.affiliation.countryisoit
hcfmusp.author.externalGADERMAIER, Gabriele:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalVEJVAR, Eva:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalHIMLY, Martin:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalMARI, Adriano:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalLISO, Marina:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalPOMPONI, Debora:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalBREITENEDER, Heimo:Med Univ Vienna, Dept Pathophysiol & Allergy Res, Ctr Pathophysiol Infectiol & Immunol, Vienna, Austria
hcfmusp.author.externalWAGNER, Stefan:Med Univ Vienna, Dept Pathophysiol & Allergy Res, Ctr Pathophysiol Infectiol & Immunol, Vienna, Austria
hcfmusp.author.externalFERREIRA, Fatima:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.citation.scopus14
hcfmusp.contributor.author-fmusphcKEITY SOUZA SANTOS
hcfmusp.contributor.author-fmusphcHELEN ANDRADE ARCURI
hcfmusp.contributor.author-fmusphcCLOVIS EDUARDO SANTOS GALVAO
hcfmusp.contributor.author-fmusphcARIANA CAMPOS YANG
hcfmusp.contributor.author-fmusphcVIRGINIA MARIA FERREIRA RESENDE
hcfmusp.contributor.author-fmusphcCARLO DE OLIVEIRA MARTINS
hcfmusp.contributor.author-fmusphcJORGE ELIAS KALIL FILHO
hcfmusp.contributor.author-fmusphcFABIO FERNANDES MORATO CASTRO
hcfmusp.description.beginpage1100
hcfmusp.description.endpage1109
hcfmusp.description.issue6
hcfmusp.description.issueSpecial Issue
hcfmusp.description.volume57
hcfmusp.origemWOS
hcfmusp.origem.pubmed23526605
hcfmusp.origem.scopus2-s2.0-84878897708
hcfmusp.origem.wosWOS:000320114800017
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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