Synbiotic Supplementation Modulates Gut Microbiota, Regulates beta-Catenin Expression and Prevents Weight Gain in ob/ob Mice: Preliminary Findings

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorDUARTE, Sebastiao Mauro B.
dc.contributor.authorSTEFANO, Jose Tadeu
dc.contributor.authorFRANCO, Lucas A. M.
dc.contributor.authorMARTINS, Roberta C.
dc.contributor.authorMORAES, Bruna D. G. C.
dc.contributor.authorBARBEIRO, Denise Frediani
dc.contributor.authorOLIVEIRA, Nathalia
dc.contributor.authorNERI, Junia Marielle Teixeira Rodrigues
dc.contributor.authorCOGLIATI, Bruno
dc.contributor.authorVANNI, Denise Siqueira
dc.contributor.authorSABINO, Ester C.
dc.contributor.authorCARRILHO, Flair J.
dc.contributor.authorOLIVEIRA, Claudia P.
dc.date.accessioned2022-10-26T14:27:08Z
dc.date.available2022-10-26T14:27:08Z
dc.date.issued2022
dc.description.abstractBackground: Obesity is one of the main health problems in the world today, and dysbiosis seems to be one of the factors involved. The aim of this study was to examine the impact of synbiotic supplementation on obesity and the microbiota in ob/ob mice. Twenty animals were divided into four groups: obese treated (OT), obese control (OC), lean treated (LT) and lean control (LC). All animals received a standard diet for 8 weeks. The treated groups received a synbiotic (Simbioflora-Invictus Farmanutricao Ltd., Sao Paulo, Brazil) in water, while the nontreated groups received only water. After 8 weeks, all animals were sacrificed, and gut tissue and stool samples were collected for mRNA isolation and microbiota analysis, respectively. beta-Catenin, occludin, cadherin and zonulin in the gut tissue were analyzed via RT-qPCR. Microbiome DNA was extracted from stool samples and sequenced using an Ion PGM Torrent platform. Results: Synbiotic supplementation reduced body weight gain in the OT group compared with the OC group (p = 0.0398) and was associated with an increase in Enterobacteriaceae (p = 0.005) and a decrease in Cyanobacteria (p = 0.047), Clostridiaceae (p = 0.026), Turicibacterales (p = 0.005) and Coprococcus (p = 0.047). On the other hand, a significant reduction in Sutterella (p = 0.009) and Turicibacter (p = 0.005) bacteria was observed in the LT group compared to the LC group. Alpha and beta diversities were different among all treated groups. beta-Catenin gene expression was significantly decreased in the gut tissue of the OT group (p <= 0.0001) compared to the other groups. No changes were observed in occludin, cadherin or zonulin gene expression in the gut tissue. Conclusions: Synbiotic supplementation prevents excessive weight gain, modulates the gut microbiota, and reduces beta-catenin expression in ob/ob mice.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, n.18, article ID 10483, 16p, 2022
dc.identifier.doi10.3390/ijms231810483
dc.identifier.eissn1422-0067
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/49241
dc.language.isoeng
dc.publisherMDPIeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rightsopenAccesseng
dc.rights.holderCopyright MDPIeng
dc.subjectgut microbiotaeng
dc.subjectsynbiotic supplementationeng
dc.subjectprobioticseng
dc.subjectprebioticseng
dc.subjectobeng
dc.subjectob miceeng
dc.subject.otherfatty liver-diseaseeng
dc.subject.otherinterventioneng
dc.subject.otheractivationeng
dc.subject.otherjunctionseng
dc.subject.otherpatternseng
dc.subject.otherobesityeng
dc.subject.otherhealtheng
dc.subject.othercancereng
dc.subject.wosBiochemistry & Molecular Biologyeng
dc.subject.wosChemistry, Multidisciplinaryeng
dc.titleSynbiotic Supplementation Modulates Gut Microbiota, Regulates beta-Catenin Expression and Prevents Weight Gain in ob/ob Mice: Preliminary Findingseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalCOGLIATI, Bruno:Univ Sao Paulo, Dept Pathol, Escola Med Vet & Ciencia Anim, BR-05508270 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus2
hcfmusp.contributor.author-fmusphcSEBASTIAO MAURO BEZERRA DUARTE
hcfmusp.contributor.author-fmusphcJOSE TADEU STEFANO
hcfmusp.contributor.author-fmusphcLUCAS AUGUSTO MOYSES FRANCO
hcfmusp.contributor.author-fmusphcROBERTA CRISTINA RUEDAS MARTINS
hcfmusp.contributor.author-fmusphcBRUNA DEL GUERRA DE CARVALHO MORAES
hcfmusp.contributor.author-fmusphcDENISE FREDIANI BARBEIRO
hcfmusp.contributor.author-fmusphcNATHALIA OLIVEIRA
hcfmusp.contributor.author-fmusphcJUNIA MARIELLE TEIXEIRA RODRIGUES NERI
hcfmusp.contributor.author-fmusphcDENISE SIQUEIRA VANNI
hcfmusp.contributor.author-fmusphcESTER CERDEIRA SABINO
hcfmusp.contributor.author-fmusphcFLAIR JOSE CARRILHO
hcfmusp.contributor.author-fmusphcCLAUDIA PINTO MARQUES SOUZA DE OLIVEIRA
hcfmusp.description.articlenumber10483
hcfmusp.description.issue18
hcfmusp.description.volume23
hcfmusp.origemWOS
hcfmusp.origem.pubmed36142396
hcfmusp.origem.scopus2-s2.0-85138361606
hcfmusp.origem.wosWOS:000858373900001
hcfmusp.publisher.cityBASELeng
hcfmusp.publisher.countrySWITZERLANDeng
hcfmusp.relation.referenceAhmad A, 2019, METABOLISM, V90, P31, DOI 10.1016/j.metabol.2018.10.004eng
hcfmusp.relation.referenceAli MS, 2019, RSC ADV, V9, P29368, DOI 10.1039/c9ra04388heng
hcfmusp.relation.referenceAnstee QM, 2006, INT J EXP PATHOL, V87, P1, DOI 10.1111/j.0959-9673.2006.00465.xeng
hcfmusp.relation.referenceAuchtung TA, 2016, GENOME ANNOUNCEMENTS, V4, DOI 10.1128/genomeA.00114-16eng
hcfmusp.relation.referenceBasu Sayon, 2018, F1000Res, V7, DOI 10.12688/f1000research.15782.1eng
hcfmusp.relation.referenceBenzler J, 2013, ENDOCRINOLOGY, V154, P4737, DOI 10.1210/en.2013-1746eng
hcfmusp.relation.referenceCampbell HK, 2017, EXP CELL RES, V358, P39, DOI 10.1016/j.yexcr.2017.03.061eng
hcfmusp.relation.referenceCaporaso JG, 2011, P NATL ACAD SCI USA, V108, P4516, DOI 10.1073/pnas.1000080107eng
hcfmusp.relation.referenceCesaro C, 2011, DIGEST LIVER DIS, V43, P431, DOI 10.1016/j.dld.2010.10.015eng
hcfmusp.relation.referenceCotillard A, 2013, NATURE, V500, P585, DOI 10.1038/nature12480eng
hcfmusp.relation.referenceDeSantis TZ, 2006, APPL ENVIRON MICROB, V72, P5069, DOI 10.1128/AEM.03006-05eng
hcfmusp.relation.referenceEdgar RC, 2010, BIOINFORMATICS, V26, P2460, DOI 10.1093/bioinformatics/btq461eng
hcfmusp.relation.referenceEverard A, 2014, ISME J, V8, P2116, DOI 10.1038/ismej.2014.45eng
hcfmusp.relation.referenceFerrario C, 2014, J NUTR, V144, P1787, DOI 10.3945/jn.114.197723eng
hcfmusp.relation.referenceGoldenberg JZ, 2013, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD006095.pub3eng
hcfmusp.relation.referenceGoretsky T, 2016, J BIOL CHEM, V291, P4166, DOI 10.1074/jbc.M115.669416eng
hcfmusp.relation.referenceHalmos T, 2016, ORVOSI HETILAP, V157, P13, DOI 10.1556/650.2015.30296eng
hcfmusp.relation.referenceHiippala K, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.01706eng
hcfmusp.relation.referenceHorie M, 2017, EXP ANIM TOKYO, V66, P405, DOI 10.1538/expanim.17-0021eng
hcfmusp.relation.referenceHouschyar KS, 2018, INT J ARTIF ORGANS, V41, P247, DOI 10.1177/0391398818762357eng
hcfmusp.relation.referenceIpsen DH, 2018, CELL MOL LIFE SCI, V75, P3313, DOI 10.1007/s00018-018-2860-6eng
hcfmusp.relation.referenceJayakumar S, 2019, ALIMENT PHARM THER, V50, P144, DOI 10.1111/apt.15314eng
hcfmusp.relation.referenceJia QJ, 2020, PHARMACOL RES, V151, DOI 10.1016/j.phrs.2019.104552eng
hcfmusp.relation.referenceJiao N, 2018, PHYSIOL GENOMICS, V50, P244, DOI 10.1152/physiolgenomics.00114.2017eng
hcfmusp.relation.referenceKaplan RC, 2019, GENOME BIOL, V20, DOI 10.1186/s13059-019-1831-zeng
hcfmusp.relation.referenceKu CS, 2013, J MED FOOD, V16, P103, DOI 10.1089/jmf.2012.2468eng
hcfmusp.relation.referenceKuczynski Justin, 2011, Curr Protoc Bioinformatics, VChapter 10, DOI [10.1002/9780471729259.mc01e05s27, 10.1002/0471250953.bi1007s36]eng
hcfmusp.relation.referenceKuugbee ED, 2016, DIGEST DIS SCI, V61, P2908, DOI 10.1007/s10620-016-4238-7eng
hcfmusp.relation.referenceLe Chatelier E, 2013, NATURE, V500, P541, DOI 10.1038/nature12506eng
hcfmusp.relation.referenceLiu JP, 2016, WORLD J GASTROENTERO, V22, P7353, DOI 10.3748/wjg.v22.i32.7353eng
hcfmusp.relation.referenceMadrid AM, 2011, DIGEST DIS SCI, V56, P155, DOI 10.1007/s10620-010-1239-9eng
hcfmusp.relation.referenceMonga SP, 2015, GASTROENTEROLOGY, V148, P1294, DOI 10.1053/j.gastro.2015.02.056eng
hcfmusp.relation.referencePedrogo DAM, 2019, INFLAMM BOWEL DIS, V25, P902, DOI 10.1093/ibd/izy318eng
hcfmusp.relation.referenceNiessen CM, 2007, J INVEST DERMATOL, V127, P2525, DOI 10.1038/sj.jid.5700865eng
hcfmusp.relation.referencePelletier RM, 2020, REPROD BIOL ENDOCRIN, V18, DOI 10.1186/s12958-020-00583-2eng
hcfmusp.relation.referenceSanders ME, 2019, NAT REV GASTRO HEPAT, V16, P605, DOI 10.1038/s41575-019-0173-3eng
hcfmusp.relation.referenceSchinner S, 2009, HORM METAB RES, V41, P159, DOI 10.1055/s-0028-1119408eng
hcfmusp.relation.referenceSchrezenmeir J, 2001, AM J CLIN NUTR, V73, p361S, DOI 10.1093/ajcn/73.2.361seng
hcfmusp.relation.referenceSekirov I, 2010, PHYSIOL REV, V90, P859, DOI 10.1152/physrev.00045.2009eng
hcfmusp.relation.referenceShao YK, 2017, OBES SURG, V27, P295, DOI 10.1007/s11695-016-2297-7eng
hcfmusp.relation.referenceShimizu H, 2015, MICROBIOL IMMUNOL, V59, P643, DOI 10.1111/1348-0421.12332eng
hcfmusp.relation.referenceShinde T, 2020, EUR J NUTR, V59, P3669, DOI 10.1007/s00394-020-02200-9eng
hcfmusp.relation.referenceSneddon LU, 2017, J EXP BIOL, V220, P3007, DOI 10.1242/jeb.147058eng
hcfmusp.relation.referenceTao JY, 2014, GASTROENTEROLOGY, V147, P690, DOI 10.1053/j.gastro.2014.05.004eng
hcfmusp.relation.referenceValdes AM, 2018, BMJ-BRIT MED J, V361, DOI 10.1136/bmj.j2179eng
hcfmusp.relation.referenceVallianou N, 2020, CURR OBES REP, V9, P179, DOI 10.1007/s13679-020-00379-weng
hcfmusp.relation.referenceVazquez-Baeza Y, 2013, GIGASCIENCE, V2, DOI 10.1186/2047-217X-2-16eng
hcfmusp.relation.referenceVelazquez KT, 2019, WORLD J HEPATOL, V11, P619, DOI 10.4254/wjh.v11.i8.619eng
hcfmusp.relation.referenceXie CC, 2019, NUTRIENTS, V11, DOI 10.3390/nu11112837eng
hcfmusp.relation.referenceYao FF, 2019, ARCH MED SCI, V15, P1336, DOI 10.5114/aoms.2019.86611eng
hcfmusp.relation.referenceZamparelli MS, 2017, UNITED EUR GASTROENT, V5, P944, DOI 10.1177/2050640617705576eng
hcfmusp.relation.referenceZheng YJ, 2021, PHYTOMEDICINE, V88, DOI 10.1016/j.phymed.2020.153455eng
hcfmusp.relation.referenceZhou ZW, 2018, FRONT ONCOL, V8, DOI 10.3389/fonc.2018.00371eng
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