Thymopoiesis in Pre- and Post-Hematopoietic Stem Cell Transplantation

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art_ROCHA_Thymopoiesis_in_Pre_and_PostHematopoietic_Stem_Cell_Transplantation_2018.PDF (3.08 MB)
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Autores
BANDEIRA, Francine
BOLLINI, Alexia
TESTA, Lucia Helena de A.
SIMIONE, Anderson Joao
SOUZA, Marina de O. e
ZANETTI, Lilian P.
OLIVEIRA, Leila Cibele S. de
SANTOS, Ana Claudia F. dos
Citação
FRONTIERS IN IMMUNOLOGY, v.9, article ID 1889, 13p, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Hematopoietic stem cell transplantation (HSCT) is an important therapeutic option for some hematological diseases. However, patients who undergo HSCT acquire a state of immunodeficiency that causes significant mortality. Reconstitution of thymic function is needed to support the immune system. One way to measure thymic function is through T-cell receptor excision circle (TREC) quantification. TRECs are generated by T-cell receptor gene rearrangements during T-cell maturation in the thymus and represent a reliable marker for thymic output. In this study, we aimed to assess aging and malignant hematological diseases as two important factors that may influence thymic output before HSCT. We observed that patients before HSCT presented signal joint TREC (sjTREC) numbers lower than 606.55 copies/mu g DNA (low values) compared with healthy individuals, with an odds ratio (OR) of 12.88 [95% confidence interval (CI): 5.26-31.53; p < 0.001]. Our results showed that a group of older individuals (>= 50 years old), comprising both healthy individuals and patients, had an OR of 10.07 (95% CI: 2.80-36.20) for low sjTREC values compared with younger individuals (<= 24 years old; p < 0.001). Multiple logistic regression analysis confirmed that both older age (>= 50 years old) and malignant hematological diseases and their treatments were important and independent risk factors related to thymic function impairment (p < 0.001). The median sjTREC value for patients of all ages was significantly lower than the sjTREC median for the subgroup of older healthy individuals (>= 50 years old; p < 0.001). These data suggested that patients before HSCT and healthy individuals exhibited age-dependent thymic impairment, and that prior treatment for hematological diseases may exacerbate aging-related deterioration of natural thymic function. Furthermore, we analyzed these patients 9 months post-HSCT and compared patients who underwent autologous HSCT with those who underwent allogeneic HSCT. Both groups of patients achieved sjTREC copy numbers similar to those of healthy individuals. We did not find a close relationship between impaired thymic function prior to HSCT and worse thymic recovery after HSCT.
Palavras-chave
T-cell receptor excision circles, T-cell receptor gene, autologous hematopoietic stem cell transplantation, allogeneic hematopoietic stem cell transplantation, malignant hematological diseases, immune reconstitution, thymopoiesis, adaptive immune system
URI
https://observatorio.fm.usp.br/handle/OPI/29337
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/19
Artigos e Materiais de Revistas Científicas - LIM/52
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