Detection of coinfection with Primate Erythroparvovirus 1 and arboviruses (DENV, CHIKV and ZIKV) in individuals with acute febrile illness in the state of Rio Grande do Norte in 2016

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMORAIS, Vanessa dos Santos
dc.contributor.authorSANTANA, Lidia Maria Reis
dc.contributor.authorBEZERRA, Joao Felipe
dc.contributor.authorCRUZ, Flavia Emmanuelle
dc.contributor.authorSOUZA, Themis Rocha de
dc.contributor.authorTAHMASEBI, Roozbeh
dc.contributor.authorRAPOSO, Rafael Augusto Alves
dc.contributor.authorMARCATTI, Roberta
dc.contributor.authorBARBOSA, Erick Matheus Garcia
dc.contributor.authorHEFFORD, Philip Michael
dc.contributor.authorBUCCHERI, Renata
dc.contributor.authorSABINO, Ester Cerdeira
dc.contributor.authorCOSTA, Antonio Charlys da
dc.date.accessioned2023-12-15T18:52:42Z
dc.date.available2023-12-15T18:52:42Z
dc.date.issued2023
dc.description.abstractBackgroundArthropod-borne viruses, known as arboviruses, pose substantial risks to global public health. Dengue (DENV), Chikungunya (CHIKV) and Zika (ZIKV) viruses stand out as significant concerns in Brazil and worldwide. Their overlapping clinical manifestations make accurate diagnosis a challenge, underscoring the need for reliable laboratory support. This study employs a comprehensive molecular diagnostic approach to track viral infections in individuals with acute febrile illness, a period marked by widespread outbreaks of DENV, CHIKV and ZIKV.MethodsBetween January and August 2016, we received a total of 713 serum samples obtained from individuals with acute febrile illness, previously tested for DENV, CHIKV or ZIKV, with initial negative results, from LACEN-NATAL. Of the total 713 samples, 667 were from females (354 of them pregnant) and 46 from males. Molecular diagnosis was conducted using the Multiplex RT-qPCR technique for simultaneous detection of DENV, CHIKV and ZIKV. Additionally, we performed differential diagnosis by RT-qPCR for other viruses of the Flavivirus, Alphavirus Enterovirus genera and qPCR for Primate Erythroparvovirus 1 (B19V) species, in accordance with Ministry of Health guidelines.ResultsAmong the 713 cases, 78.2% tested positive for viral infections, including 48% with CHIKV viremia, 0.6% with DENV and 0.1% with ZIKV. Arboviral coinfections totaled 2.4%, including DENV-CHIKV (1.7%) and CHIKV-ZIKV (0.7%). Moreover, 8% exhibited B19V viremia. Simultaneous infections were identified in 17.5%, encompassing B19V-CHIKV (17.1%), B19V-DENV (0.1%), and B19V-ZIKV (0.3%) Triple infections were observed in 1.3% of cases with B19V-DENV-CHIKV (1%) and B19V-CHIKV-ZIKV (0.3%).ConclusionMolecular testing demonstrated high efficacy in diagnosing prevalent arboviruses and detecting multiple coinfections. This approach helps to elucidate etiologies for symptomatic cases, especially during arbovirus outbreaks, and aids comprehensive surveillance. Our findings underscore the importance of monitoring co-circulating pathogens, such as B19V, with implications for clinical management, particularly in pregnant individuals. This study enhances our understanding of arbovirus epidemiology and reinforces the critical role of molecular diagnosis in disease surveillance and control.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.sponsorshipWe thank the Central Public Health Laboratory (LACEN-RN) and the Guamare Health Department for their support in the production of this manuscript. To the Ministry of Health for donating the reagents used in this study.
dc.identifier.citationPLOS NEGLECTED TROPICAL DISEASES, v.17, n.11, article ID e0011701, 14p, 2023
dc.identifier.doi10.1371/journal.pntd.0011701
dc.identifier.issn1935-2735
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/57441
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCEeng
dc.relation.ispartofPlos Neglected Tropical Diseases
dc.rightsopenAccesseng
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCEeng
dc.subject.otherparvovirus b19 infectioneng
dc.subject.otherpregnancyeng
dc.subject.otheroutbreakeng
dc.subject.otherbrazileng
dc.subject.otherviruseng
dc.subject.otherurbaneng
dc.subject.wosInfectious Diseaseseng
dc.subject.wosParasitologyeng
dc.subject.wosTropical Medicineeng
dc.titleDetection of coinfection with Primate Erythroparvovirus 1 and arboviruses (DENV, CHIKV and ZIKV) in individuals with acute febrile illness in the state of Rio Grande do Norte in 2016eng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryInglaterra
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisogb
hcfmusp.affiliation.countryisous
hcfmusp.author.externalSANTANA, Lidia Maria Reis:Epidemiol Surveillance Ctr Prof Alexandre Vranjac, Sao Paulo Hlth Dept, Sao Paulo, Brazil; Univ Fed Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalBEZERRA, Joao Felipe:Univ Fed Paraiba, Joao Pessoa, Paraiba, Brazil
hcfmusp.author.externalCRUZ, Flavia Emmanuelle:Guamare Hlth Dept, Guamare, Brazil
hcfmusp.author.externalSOUZA, Themis Rocha de:Cent Publ Hlth Lab State Rio Grande Do Norte, Natal, Brazil
hcfmusp.author.externalTAHMASEBI, Roozbeh:Univ Sao Paulo, Dept Infect & Parasit Dis, Sao Paulo, Brazil
hcfmusp.author.externalRAPOSO, Rafael Augusto Alves:Univ Sao Paulo, Dept Infect & Parasit Dis, Sao Paulo, Brazil
hcfmusp.author.externalHEFFORD, Philip Michael:Univ Hosp Sussex NHS Fdn Trust, Chichester, England
hcfmusp.author.externalBUCCHERI, Renata:Vitalant Res Inst, Dept Epidemiol, San Francisco, CA USA
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcVANESSA DOS SANTOS MORAIS
hcfmusp.contributor.author-fmusphcROBERTA MARCATTI DE AZEVEDO
hcfmusp.contributor.author-fmusphcERICK MATHEUS GARCIA BARBOSA
hcfmusp.contributor.author-fmusphcESTER CERDEIRA SABINO
hcfmusp.contributor.author-fmusphcANTONIO CHARLYS DA COSTA
hcfmusp.description.articlenumbere0011701
hcfmusp.description.issue11
hcfmusp.description.volume17
hcfmusp.origemWOS
hcfmusp.origem.pubmed37917659
hcfmusp.origem.scopus2-s2.0-85176973614
hcfmusp.origem.wosWOS:001099906400001
hcfmusp.publisher.citySAN FRANCISCOeng
hcfmusp.publisher.countryUSAeng
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