The potential use of stem cells derived from human amniotic fluid in renal diseases

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorNORONHA, Irene L.
dc.contributor.authorCAVAGLIERI, Rita C.
dc.contributor.authorJANZ, Felipe L.
dc.contributor.authorDUARTE, Sergio A.
dc.contributor.authorLOPES, Marco A. B.
dc.contributor.authorZUGAIB, Marcelo
dc.contributor.authorBYDLOWSKI, Sergio P.
dc.date.accessioned2017-11-27T16:23:32Z
dc.date.available2017-11-27T16:23:32Z
dc.date.issued2011
dc.description.abstractAmniotic fluid (AF) contains a variety of cell types derived from fetal tissues that can easily grow in culture. These cells can be obtained during amniocentesis for prenatal screening of fetal genetic diseases, usually performed during the second trimester of pregnancy. Of particular interest, some expanded sub-populations derived from AF cells are capable of extensive self-renewal and maintain prolonged undifferentiated proliferation, which are defining properties of stem cells. These human AF stem cells (hAFSCs) exhibit multilineage potential and can differentiate into the three germ layers. They have high proliferation rates and express mesenchymal and embryonic markers, but do not induce tumor formation. In this study, hAFSCs derived from amniocentesis performed at 16-20 weeks of pregnancy were isolated, grown in culture, and characterized by flow cytometry and by their potential ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. After 4-7 passages, 5 x 10(5) hAFSCs were inoculated under the kidney capsule of Wistar rats that were subjected to an experimental model of chronic renal disease, the 5/6 nephrectomy model (Nx). After 30 days, Nx rats treated with hAFSCs displayed significant reductions in blood pressure, proteinuria, macrophages, and a-smooth muscle actin expression compared with Nx animals. These preliminary results suggest that hAFSCs isolated and expanded from AF obtained by routine amniocentesis can promote renoprotection in the Nx model. Considering that the AF cells not used for fetal karyotyping are usually discarded, and that their use does not raise ethical issues, they may represent an alternative source of stem cells for cell therapy and regenerative medicine.
dc.description.indexPubMed
dc.identifier.citationKIDNEY INTERNATIONAL SUPPLEMENTS, v.1, n.3, p.77-82, 2011
dc.identifier.doi10.1038/kisup.2011.18
dc.identifier.issn2157-1724
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/22616
dc.language.isoeng
dc.publisherNATURE PUBLISHING GROUP
dc.relation.ispartofKidney International Supplements
dc.rightsrestrictedAccess
dc.rights.holderCopyright NATURE PUBLISHING GROUP
dc.subjectamniotic fluid stem cells
dc.subjectchronic kidney disease
dc.subjectmesenchymal stem cells
dc.subject.wosUrology & Nephrology
dc.titleThe potential use of stem cells derived from human amniotic fluid in renal diseases
dc.typearticle
dc.type.categoryreview
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalDUARTE, Sergio A.:Univ Sao Paulo, Dept Obstet & Gynecol, BR-01246903 Sao Paulo, Brazil
hcfmusp.citation.scopus9
hcfmusp.contributor.author-fmusphcIRENE DE LOURDES NORONHA
hcfmusp.contributor.author-fmusphcRITA DE CASSIA CAVAGLIERI
hcfmusp.contributor.author-fmusphcFELIPE DE LARA JANZ
hcfmusp.contributor.author-fmusphcMARCO ANTONIO BORGES LOPES
hcfmusp.contributor.author-fmusphcMARCELO ZUGAIB
hcfmusp.contributor.author-fmusphcSERGIO PAULO BYDLOWSKI
hcfmusp.description.beginpage77
hcfmusp.description.endpage82
hcfmusp.description.issue3
hcfmusp.description.volume1
hcfmusp.origemWOS
hcfmusp.origem.pubmed25028628
hcfmusp.origem.scopus2-s2.0-84884562396
hcfmusp.origem.wosWOS:000208711000004
hcfmusp.publisher.cityNEW YORK
hcfmusp.publisher.countryUSA
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