Protoporphyrin fluorescence induced by methyl-ALA in skin healing

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorGONDIM, Roberta M. F.
dc.contributor.authorVIEIRA, Vinicius C. C.
dc.contributor.authorVERAS, Mariana M.
dc.contributor.authorFERREIRA, Marcelo A.
dc.contributor.authorCALDINI, Elia Tamaso Espin Garcia
dc.contributor.authorMUNOZ, Daniel Romero
dc.contributor.authorBAPTISTA, Mauricio S.
dc.date.accessioned2014-04-25T21:54:34Z
dc.date.available2014-04-25T21:54:34Z
dc.date.issued2013
dc.description.abstractBackground: Estimation of the time period that precedes an injury is critical in forensic medicine. However, there is no reliable method that can be used to evaluate the oldness of a lesion. The aim of this work is to develop a fluorimetric method that can be used to follow the aging process of lesions by applying methyl ALA (MAL) on wounds and by quantifying protoporphyrin IX (PPIX) fluorescence during the healing process. We also aim to understand the changes in PPIX fluorescence by establishing a correlation with histological evaluations during the heating process. Methods: Standardized linear wounds were made on the dorsum of 72 mice, which were divided in control (MAL -) and experimental (MAL +) groups. In vivo fluorescence spectra (FS) were collected from normal and wound skin sites of control and experimental groups, corresponding to four groups of FS spectra: (a) FS of skin wound after MAL (+/+); (b) FS of normal skin after MAL (-/+); (c) FS of skin wound without MAL (+/-) and (d) FS of normal skin without MAL (-/-). Animals were monitored periodically for 3 months and euthanized. Tissue specimens were processed for histological analysis using design-based stereological methods. Serial cross-sections were analyzed to evaluate the organization of the dermis and epidermis, collagen deposition and cellular proliferation. Results: FS of skin wound with MAL (+/+) showed an expressive intensity increase from the beginning of the experiment to the 34th day, with maximum fluorescence being observed on the similar to 11th day after wounding. There was preferential PPIX accumulation in healing sites as compared to adjacent normal skin (+/-) in the early stage of healing. Histological findings allowed correlation of the fluorescence increase mainly with cell proliferation. The drastic decrease in the FS intensity observed in the end of the healing process was correlated with the decrease in the proliferation rate as well as with the presence of new extracellular fibrous materials. Conclusions: In the mice wound-healing model tested here, it was possible to distinguish whether the injury was in early or advanced stages by using PPIX fluorescence induced by MAL. We conclude that this method is a promising approach to evaluate the age of skin wounding and we hope this work will stimulate human studies to allow this technique to become standardized in forensic medicine.
dc.description.indexMEDLINE
dc.identifier.citationPHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, v.10, n.4, p.389-398, 2013
dc.identifier.doi10.1016/j.pdpdt.2013.05.005
dc.identifier.eissn1873-1597
dc.identifier.issn1572-1000
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5178
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER SCIENCE BV
dc.subjectSpectroscopy
dc.subjectLesion age
dc.subjectTraumatic lesion
dc.subjectExperimental surgery
dc.subjectDermatology
dc.subjectLegal medicine
dc.subject.otherbasal-cell carcinoma
dc.subject.other5-aminolevulinic acid
dc.subject.othertopical application
dc.subject.otherphotodynamic therapy
dc.subject.otherin-vivo
dc.subject.otherreflectance spectroscopy
dc.subject.otherwound age
dc.subject.otheraminolevulinate
dc.subject.otherdiagnosis
dc.subject.otherester
dc.subject.wosOncology
dc.titleProtoporphyrin fluorescence induced by methyl-ALA in skin healing
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalVIEIRA, Vinicius C. C.:Univ Sao Paulo, Inst Quim, BR-05508 Sao Paulo, Brazil
hcfmusp.author.externalBAPTISTA, Mauricio S.:Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508 Sao Paulo, Brazil
hcfmusp.citation.scopus4
hcfmusp.contributor.author-fmusphcROBERTA MARINHO FALCAO GONDIM
hcfmusp.contributor.author-fmusphcMARIANA MATERA VERAS
hcfmusp.contributor.author-fmusphcMARCELO ALVES FERREIRA
hcfmusp.contributor.author-fmusphcELIA TAMASO ESPIN GARCIA CALZOLARI
hcfmusp.contributor.author-fmusphcDANIEL ROMERO MUñOZ
hcfmusp.description.beginpage389
hcfmusp.description.endpage398
hcfmusp.description.issue4
hcfmusp.description.volume10
hcfmusp.origemWOS
hcfmusp.origem.pubmed24284091
hcfmusp.origem.scopus2-s2.0-84888028026
hcfmusp.origem.wosWOS:000328666900010
hcfmusp.publisher.cityAMSTERDAM
hcfmusp.publisher.countryNETHERLANDS
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