Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
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Citações na Scopus
9
Tipo de produção
article
Data de publicação
2017
Título da Revista
ISSN da Revista
Título do Volume
Editora
HOSPITAL CLINICAS, UNIV SAO PAULO
Autores
GIANNOCCO, Gisele
SILVA, Ismael Dale Cotrim Guerreiro da
MALIQUEO, Manuel
Citação
CLINICS, v.72, n.8, p.510-514, 2017
Resumo
OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats.
Palavras-chave
Polycystic Ovary Syndrome, Hypothalamus, Animal Models, Kisspeptin, Testosterone
Referências
- Alexanderson C, 2007, ENDOCRINOLOGY, V148, P5369, DOI 10.1210/en.2007-0305
- DiVall SA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0119995
- Dumesic DA, 2015, ENDOCR REV, V36, P487, DOI 10.1210/er.2015-1018
- Feng Y, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0006638
- Foecking EM, 2005, BIOL REPROD, V72, P1475, DOI 10.1095/biolreprod.105.039800
- Goodarzi MO, 2011, NAT REV ENDOCRINOL, V7, P219, DOI 10.1038/nrendo.2010.217
- Herbison AE, 2016, NAT REV ENDOCRINOL, V12, P452, DOI 10.1038/nrendo.2016.70
- Hiroi R, 2013, ENDOCRINOLOGY, V154, P1802, DOI 10.1210/en.2012-2253
- Lehman MN, 2010, ENDOCRINOLOGY, V151, P3479, DOI 10.1210/en.2010-0022
- Mahamed RR, 2011, FERTIL STERIL, V95, P1507, DOI 10.1016/j.fertnstert.2010.07.1093
- Marcondes RR, 2015, GEN COMP ENDOCR, V212, P28, DOI 10.1016/j.ygcen.2015.01.006
- Pielecka J, 2006, ENDOCRINOLOGY, V147, P1474, DOI 10.1210/en.2005-1029
- Radovick Sally, 2012, Front Endocrinol (Lausanne), V3, P52, DOI 10.3389/fendo.2012.00052
- Roa J, 2008, ENDOCRINOLOGY, V149, P1627, DOI 10.1210/en.2007-1540
- Sun JL, 2010, ENDOCRINOLOGY, V151, P5349, DOI 10.1210/en.2010-0385
- Topaloglu AK, 2009, NAT GENET, V41, P354, DOI 10.1038/ng.306
- Walters KA, 2012, BIOL REPROD, V86, DOI 10.1095/biolreprod.111.097808
- Wintermantel TM, 2006, NEURON, V52, P271, DOI 10.1016/j.neuron.2006.07.023
- Yan XN, 2014, J ENDOCRINOL, V222, P73, DOI 10.1530/JOE-14-0021