Proficiency of DNA repair genes and microsatellite instability in operated colorectal cancer patients with clinical suspicion of lynch syndrome

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFREITAS, Isabella Nicacio de
dc.contributor.authorCAMPOS, Fabio Guilherme Caserta Maryssael de
dc.contributor.authorALVES, Venancio Avancini Ferreira
dc.contributor.authorCAVALCANTE, Juliana Magalhaes
dc.contributor.authorCARRARO, Dirce
dc.contributor.authorCOUDRY, Renata de Almeida
dc.contributor.authorDINIZ, Marcio Augusto
dc.contributor.authorNAHAS, Sergio Carlos
dc.contributor.authorRIBEIRO JR., Ulysses
dc.date.accessioned2016-07-04T13:58:30Z
dc.date.available2016-07-04T13:58:30Z
dc.date.issued2015
dc.description.abstractBackground: Lynch syndrome (LS) diagnosis is underestimated, and most of the patients remain undetected after colorectal resections. The study aims to assess the frequency of LS in patients undergoing surgical treatment for colorectal cancer (CRC). Methods: A total of 458 CRC patients were operated from January 2005 to December 2008. Positive CRC family history (FH) was present in 118 (25.8%) patients. Histologic sections were reviewed for microsatellite instability (MSI) criteria (Bethesda guidelines), immunohistochemical (IHC) analysis for MLH1, MSH2, MSH6, PMS2 proteins, through the avidin-biotin-peroxidase complex, MSI (BAT-25, BAT-26, NR-21, NR-24 and MONO-27) and BRAF somatic mutation. Results: Of the 118 patients with FH, 61 (51.69%) met at least one of the revised Bethesda criteria. IHC was abnormal in 8 (13.1%) and MSI in 12 patients (20%). BRAF was negative in all cases. MSI histopathological included: intratumoral lymphocytes (47.5%), expansive tumors (29.5%) mucinous component (27.8%) and Crohn's like reaction in (14.7%). There was an association between the revised Bethesda criteria with: sex, mucinous histology and Crohn's like reaction; MSI and IHC with PMS2 and MLH1. Revised Bethesda criteria 4 had 10.6 increased chances to display positive MSI. We have proposed a score to contribute as a practical tool in the diagnosis of LS. Conclusions: The frequence of LS in resected CRC patients was 2.6%. The criterion 4 Revised Bethesda was associated more strongly with the presence of MSI.
dc.description.indexPubMed
dc.identifier.citationJOURNAL OF GASTROINTESTINAL ONCOLOGY, v.6, n.6, p.628-637, 2015
dc.identifier.doi10.3978/j.issn.2078-6891.2015.089
dc.identifier.eissn2219-679X
dc.identifier.issn2078-6891
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/13981
dc.language.isoeng
dc.publisherPIONEER BIOSCIENCE PUBL CO
dc.relation.ispartofJournal of Gastrointestinal Oncology
dc.rightsopenAccess
dc.rights.holderCopyright PIONEER BIOSCIENCE PUBL CO
dc.subjectHereditary nonpolyposis colorectal cancer (HNPCC)
dc.subjectimmunohistochemical (IHC)
dc.subjectlynch syndrome (LS)
dc.subjectmicrosatellite instability (MSI)
dc.subjectB-raf
dc.subject.otherrevised bethesda guidelines
dc.subject.otherhnpcc
dc.subject.otherrisk
dc.subject.otheridentification
dc.subject.othermutations
dc.subject.otherimmunohistochemistry
dc.subject.othercarcinomas
dc.subject.othermanagement
dc.subject.otherpathology
dc.subject.otherfeatures
dc.subject.wosOncology
dc.titleProficiency of DNA repair genes and microsatellite instability in operated colorectal cancer patients with clinical suspicion of lynch syndrome
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalCAVALCANTE, Juliana Magalhaes:Hosp AC Camargo Fund Antonio Prudente, Sao Paulo, SP, Brazil
hcfmusp.author.externalCARRARO, Dirce:Hosp AC Camargo Fund Antonio Prudente, Sao Paulo, SP, Brazil
hcfmusp.author.externalCOUDRY, Renata de Almeida:Hosp AC Camargo Fund Antonio Prudente, Sao Paulo, SP, Brazil
hcfmusp.author.externalDINIZ, Marcio Augusto:Univ Sao Paulo, Sch Med, Dept Gastroenterol & Pathol, Sao Paulo, SP, Brazil
hcfmusp.citation.scopus8
hcfmusp.contributor.author-fmusphcISABELLA NICACIO DE FREITAS
hcfmusp.contributor.author-fmusphcFABIO GUILHERME CASERTA MARYSSAEL DE CAMPOS
hcfmusp.contributor.author-fmusphcVENANCIO AVANCINI FERREIRA ALVES
hcfmusp.contributor.author-fmusphcSERGIO CARLOS NAHAS
hcfmusp.contributor.author-fmusphcULYSSES RIBEIRO JUNIOR
hcfmusp.description.beginpage628
hcfmusp.description.endpage637
hcfmusp.description.issue6
hcfmusp.description.volume6
hcfmusp.origemWOS
hcfmusp.origem.pubmed26697194
hcfmusp.origem.scopus2-s2.0-84995768017
hcfmusp.origem.wosWOS:000366908400007
hcfmusp.publisher.cityHONG KONG
hcfmusp.publisher.countryPEOPLES R CHINA
hcfmusp.relation.referenceHampel H, 2005, NEW ENGL J MED, V352, P1851, DOI 10.1056/NEJMoa043146
hcfmusp.relation.referenceGologan A, 2005, ARCH PATHOL LAB MED, V129, P1390
hcfmusp.relation.referencePerez-Carbonell L, 2012, GUT, V61, P865, DOI 10.1136/gutjnl-2011-300041
hcfmusp.relation.referencede la Chapelle A, 2004, NAT REV CANCER, V4, P769, DOI 10.1038/nrc1453
hcfmusp.relation.referenceZhang LY, 2008, J MOL DIAGN, V10, P301, DOI 10.2353/jmoldx.2008.080062
hcfmusp.relation.referenceWolf B, 2006, INT J CANCER, V118, P1465, DOI 10.1002/ijc.21524
hcfmusp.relation.referenceHendriks YMC, 2006, CA-CANCER J CLIN, V56, P213
hcfmusp.relation.referenceKoehler-Santos P, 2011, WORLD J GASTROENTERO, V17, P766, DOI 10.3748/wjg.v17.i6.766
hcfmusp.relation.referenceVasen HFA, 1999, GASTROENTEROLOGY, V116, P1453, DOI 10.1016/S0016-5085(99)70510-X
hcfmusp.relation.referenceRodriguezBigas MA, 1997, J NATL CANCER I, V89, P1758, DOI 10.1093/jnci/89.23.1758
hcfmusp.relation.referenceKastrinos F, 2011, GASTROENTEROLOGY, V140, P73, DOI 10.1053/j.gastro.2010.08.021
hcfmusp.relation.referenceLYNCH HT, 1966, ARCH INTERN MED, V117, P206, DOI 10.1001/archinte.117.2.206
hcfmusp.relation.referenceVASEN HF, 1993, DIS COLON RECTUM, V36, P1, DOI 10.1007/BF02050292
hcfmusp.relation.referenceVASEN HFA, 1991, DIS COLON RECTUM, V34, P424, DOI 10.1007/BF02053699
hcfmusp.relation.referenceGoecke T, 2006, J CLIN ONCOL, V24, P4285, DOI 10.1200/JCO.2005.03.7333
hcfmusp.relation.referenceTruta B, 2008, FAM CANCER, V7, P267, DOI 10.1007/s10689-008-9186-8
hcfmusp.relation.referenceJenkins MA, 2007, GASTROENTEROLOGY, V133, P48, DOI 10.1053/j.gastro.2007.04.044
hcfmusp.relation.referenceSerrano M, 2012, FAM CANCER, V11, P571, DOI 10.1007/s10689-012-9550-6
hcfmusp.relation.referenceLynch HT, 2007, J NATL CANCER I, V99, P261, DOI 10.1093/jnci/djk077
hcfmusp.relation.referenceJASS JR, 1994, ANTICANCER RES, V14, P1631
hcfmusp.relation.referenceKastrinos F, 2012, J CLIN ONCOL, V30, P1024, DOI 10.1200/JCO.2011.40.7171
hcfmusp.relation.referencePinol V, 2005, JAMA-J AM MED ASSOC, V293, P1986, DOI 10.1001/jama.293.16.1986
hcfmusp.relation.referenceLynch HT, 1996, CANCER, V78, P1149, DOI 10.1002/(SICI)1097-0142(19960915)78:6<1149::AID-CNCR1>3.0.CO;2-5
hcfmusp.relation.referenceLee HJ, 2010, AM J CLIN PATHOL, V133, P802, DOI 10.1309/AJCPO3F2ENKMDTUS
hcfmusp.relation.referenceKakar S, 2004, MODERN PATHOL, V17, P696, DOI 10.1038/modpathol.3800093
hcfmusp.relation.referenceUmar A, 2004, J NATL CANCER I, V96, P261, DOI 10.1093/jnci/djh034
hcfmusp.relation.referencePalomaki GE, 2009, GENET MED, V11, P42, DOI 10.1097/GIM.0b013e31818fa2db
hcfmusp.relation.referenceUrso E, 2008, INT J COLORECTAL DIS, V23, P801, DOI 10.1007/s00384-008-0484-2
hcfmusp.relation.referenceFerreira S, 2009, ACTA MEDICA PORT, V22, P207
hcfmusp.relation.referenceHampel H, 2008, J CLIN ONCOL, V26, P5783, DOI 10.1200/JCO.2008.17.5950
hcfmusp.relation.referenceGrover S, 2010, J NATL COMPR CANC NE, V8, P98
hcfmusp.relation.referenceWright CL, 2003, AM J SURG PATHOL, V27, P1393, DOI 10.1097/00000478-200311000-00001
hcfmusp.relation.referenceLynch HT, 2003, NEW ENGL J MED, V348, P919
hcfmusp.scopus.lastupdate2024-05-17
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