Lipid core nanoparticles resembling low-density lipoprotein and regression of atherosclerotic lesions: effects of particle size
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Citações na Scopus
8
Tipo de produção
article
Data de publicação
2018
Título da Revista
ISSN da Revista
Título do Volume
Editora
ASSOC BRAS DIVULG CIENTIFICA
Citação
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.51, n.3, article ID e7090, 8p, 2018
Resumo
Particles are usually polydispersed and size is an important feature for lipid-based drug delivery systems in order to optimize cell-particle interactions as to pharmacologic action and toxicity. Lipid nanoparticles (LDE) with composition similar to that of low-density lipoprotein carrying paclitaxel were shown to markedly reduce atherosclerosis lesions induced in rabbits by cholesterol feeding. The aim of this study was to test whether two LDE fractions, one with small (20-60 nm) and the other with large (60-100 nm) particles, had different actions on the atherosclerotic lesions. The two LDE-paclitaxel fractions, prepared by microfluidization, were separated by density gradient ultracentrifugation and injected (4 mg/body weight, intravenously once a week) into two groups of rabbits previously fed cholesterol for 4 weeks. A group of cholesterol-fed animals injected with saline solution was used as control to assess lesion reduction with treatment. After the treatment period, the animals were euthanized for analysis. After treatment, both the small and large nanoparticle preparations of LDE-paclitaxel had equally strong anti-atherosclerosis action. Both reduced lesion extension in the aorta by roughly 50%, decreased the intima width by 75% and the macrophage presence in the intima by 50%. The two preparations also showed similar toxicity profile. In conclusion, within the 20-100 nm range, size is apparently not an important feature regarding the LDE nanoparticle system and perhaps other solid lipid-based systems.
Palavras-chave
Solid lipid nanoparticles, Drug targeting, Paclitaxel, Atherosclerosis, Particle size
Referências
- Ades A, 2001, GYNECOL ONCOL, V82, P84, DOI 10.1006/gyno.2001.6203
- Azevedo CHM, 2005, GYNECOL ONCOL, V97, P178, DOI 10.1016/j.ygyno.2004.12.015
- Boverhof DR, 2015, REGUL TOXICOL PHARM, V73, P137, DOI 10.1016/j.yrtph.2015.06.001
- Bulgarelli A, 2012, J CARDIOVASC PHARM, V59, P308, DOI 10.1097/FJC.0b013e318241c385
- Champion JA, 2008, PHARM RES-DORDR, V25, P1815, DOI 10.1007/s11095-008-9562-y
- Dias MLN, 2007, CANCER CHEMOTH PHARM, V59, P105, DOI 10.1007/s00280-006-0252-3
- Dorlhiac-Llacer PE, 2001, BRAZ J MED BIOL RES, V34, P1257, DOI 10.1590/S0100-879X2001001000004
- Feng L, 2013, CANCER LETT, V334, P157, DOI 10.1016/j.canlet.2012.07.006
- Goldstein JL, 2009, ARTERIOSCL THROM VAS, V29, P431, DOI 10.1161/ATVBAHA.108.179564
- Graziani SR, 2002, GYNECOL ONCOL, V85, P493, DOI 10.1006/gyno.2002.6654
- He CB, 2010, BIOMATERIALS, V31, P3657, DOI 10.1016/j.biomaterials.2010.01.065
- Hungria VTM, 2004, CANCER CHEMOTH PHARM, V53, P51, DOI 10.1007/s00280-003-0692-y
- Jiang LQ, 2017, INT J NANOMED, V12, P6383, DOI 10.2147/IJN.S142060
- Kathe N, 2014, DRUG DEV IND PHARM, V40, P1565, DOI 10.3109/03639045.2014.909840
- Kelly C, 2011, J DRUG DELIV, DOI 10.1155/2011/727241
- Kretzer IF, 2016, INT J NANOMED, V11, P885, DOI 10.2147/IJN.S88546
- Lameijer MA, 2013, EXPERT REV MOL DIAGN, V13, P567, DOI 10.1586/14737159.2013.819216
- Maranhao RC, 2008, ATHEROSCLEROSIS, V197, P959, DOI 10.1016/j.atherosclerosis.2007.12.051
- Maranhao RC, 2015, EXPERT OPIN DRUG DEL, V12, P1135, DOI 10.1517/17425247.2015.999663
- MARANHAO RC, 1994, CANCER RES, V54, P4660
- Maranhao RC, 2002, CANCER CHEMOTH PHARM, V49, P487, DOI 10.1007/s00280-002-0437-3
- Mendes S, 2009, GYNECOL ONCOL, V112, P400, DOI 10.1016/j.ygyno.2008.10.018
- Moura JA, 2011, INT J NANOMED, V6, P2285, DOI 10.2147/IJN.S18039
- Namdee K, 2014, ATHEROSCLEROSIS, V237, P279, DOI 10.1016/j.atherosclerosis.2014.09.025
- Naoum FA, 2004, AM J HEMATOL, V77, P340, DOI 10.1002/ajh.20206
- Occhiutto Marcelo L, 2012, Pharmaceutics, V4, P252, DOI 10.3390/pharmaceutics4020252
- Palombo M, 2014, ANNU REV PHARMACOL, V54, P581, DOI 10.1146/annurev-pharmtox-010611-134615
- Pinheiro KV, 2006, CANCER CHEMOTH PHARM, V57, P624, DOI 10.1007/s00280-005-0090-8
- Pires L, 2009, CANCER CHEMOTH PHARM, V63, P281, DOI 10.1007/s00280-008-0738-2
- Psarros C, 2012, NANOMED-NANOTECHNOL, V8, pS59, DOI 10.1016/j.nano.2012.05.006
- REDGRAVE TG, 1975, ANAL BIOCHEM, V65, P42, DOI 10.1016/0003-2697(75)90488-1
- Rodrigues DG, 2005, CANCER CHEMOTH PHARM, V55, P565, DOI 10.1007/s00280-004-0930-y
- Rodrigues DG, 2002, J PHARM PHARMACOL, V54, P765, DOI 10.1211/0022357021779104
- Sacks FM, 2015, CURR OPIN LIPIDOL, V26, P56, DOI 10.1097/MOL.0000000000000146
- Sercombe L, 2015, FRONT PHARMACOL, V6, DOI 10.3389/fphar.2015.00286
- Setyawati MI, 2015, CHEM SOC REV, V44, P8174, DOI 10.1039/c5cs00499c
- Teixeira RS, 2004, J PHARM PHARMACOL, V56, P909, DOI 10.1211/0022357023826
- Valduga CJ, 2003, J PHARM PHARMACOL, V55, P1615, DOI 10.1211/0022357022232
- Wissing SA, 2004, ADV DRUG DELIVER REV, V56, P1257, DOI 10.1016/j.addr.2003.12.002