Genetic differences of dengue virus 2 in patients with distinct clinical outcome
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Citações na Scopus
1
Tipo de produção
article
Data de publicação
2023
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
MARQUES, Beatriz de Carvalho
SACCHETTO, Livia
BANHO, Cecilia Artico
ESTOFOLETE, Cassia Fernanda
DOURADO, Fernanda Simoes
CANDIDO, Darlan da Silva
DUTRA, Karina Rocha
Citação
BRAZILIAN JOURNAL OF MICROBIOLOGY, v.54, n.3, p.1411-1419, 2023
Resumo
The genetic diversity of the dengue virus is characterized by four circulating serotypes, several genotypes, and an increasing number of existing lineages that may have differences in the potential to cause epidemics and disease severity. Accurate identification of the genetic variability of the virus is essential to identify lineages responsible for an epidemic and understanding the processes of virus spread and virulence. Here, we characterize, using portable nanopore genomic sequencing, different lineages of dengue virus 2 (DENV-2) detected in 22 serum samples from patients with and without dengue warning signs attended at Hospital de Base of Sao Jose do Rio Preto (SJRP) in 2019, during a DENV-2 outbreak. Demographic, epidemiological, and clinical data were also analyzed. The phylogenetic reconstruction and the clinical data showed that two lineages belonging to the American/Asian genotype of DENV-2-BR3 and BR4 (BR4L1 and BR4L2)-were co-circulating in SJRP. Although preliminary, these results indicate no specific association between clinical form and phylogenetic clustering at the virus consensus sequence level. Studies with larger sample sizes and which explore single nucleotide variants are needed. Therefore, we showed that portable nanopore genome sequencing could generate quick and reliable sequences for genomic surveillance to monitor viral diversity and its association with disease severity as an epidemic unfolds.
Palavras-chave
Genomic surveillance, Epidemic, DENV-2, Dengue severity, Sequencing, Brazil
Referências
- Bhatt P, 2021, CURR MICROBIOL, V78, P17, DOI 10.1007/s00284-020-02284-w
- Bhatt S, 2013, NATURE, V496, P504, DOI 10.1038/nature12060
- Carneiro AR, 2012, MEM I OSWALDO CRUZ, V107, P805, DOI 10.1590/S0074-02762012000600016
- Chen RB, 2011, VIRUSES-BASEL, V3, P1562, DOI 10.3390/v3091562
- Chiaravalloti NF., 1997, DESCRIPTION AEDES AE
- Chin-inmanu K, 2023, ASIAN PAC J ALLERGY, V41, P361, DOI [10.12932/ap-230620-0887, 10.12932/AP-230620-0887]
- Cidade Brasil, 2021, MUN SAO JOS RIO PRET
- Das Manandhar K, 2021, AM J TROP MED HYG, V104, P115, DOI 10.4269/ajtmh.20-0163
- de Bruycker-Nogueira F, 2016, INFECT GENET EVOL, V45, P454, DOI 10.1016/j.meegid.2016.09.025
- de Jesus JG, 2020, MEM I OSWALDO CRUZ, V115, DOI 10.1590/0074-02760190423
- Drumond BP, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0059422
- Faria NR, 2018, SCIENCE, V361, P894, DOI 10.1126/science.aat7115
- Faria NR, 2017, NATURE, V546, P406, DOI 10.1038/nature22401
- Fonseca V, 2019, PLOS NEGLECT TROP D, V13, DOI 10.1371/journal.pntd.0007231
- Nunes PCG, 2016, J MED VIROL, V88, P1130, DOI 10.1002/jmv.24464
- Hill SC, 2020, PLOS PATHOG, V16, DOI 10.1371/journal.ppat.1008699
- Hill SC, 2019, EMERG INFECT DIS, V25, P784, DOI 10.3201/eid2504.180958
- Instituto Brasileiro de Geografia e Estatistica, 2022, SAO JOS RIO PRET
- Johnson BW, 2005, J CLIN MICROBIOL, V43, P4977, DOI 10.1128/JCM.43.10.4977-4983.2005
- Kurane Ichiro, 2011, Trop Med Health, V39, P45, DOI 10.2149/tmh.2011-S09
- Larsson A, 2014, BIOINFORMATICS, V30, P3276, DOI 10.1093/bioinformatics/btu531
- Li H, 2009, BIOINFORMATICS, V25, P1094, DOI [10.1093/bioinformatics/btp100, 10.1093/bioinformatics/btp324]
- Lindenbach BD, 2013, FIELDS VIROLOGY, P712
- Loman NJ, 2015, NAT METHODS, V12, P733, DOI [10.1038/NMETH.3444, 10.1038/nmeth.3444]
- Mir Daiana, 2014, PLoS One, V9, pe98519, DOI 10.1371/journal.pone.0098519
- Modhiran N, 2015, SCI TRANSL MED, V7, DOI 10.1126/scitranslmed.aaa3863
- Mondini A, 2009, PLOS NEGLECT TROP D, V3, DOI 10.1371/journal.pntd.0000448
- Nunes MRT, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0002769
- Oliveira MF, 2010, EMERG INFECT DIS, V16, P576, DOI 10.3201/eid1603.090996
- Patro ARK, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11010034
- Quick J, 2017, NAT PROTOC, V12, P1261, DOI 10.1038/nprot.2017.066
- Rocco IM, 2012, REV INST MED TROP SP, V54, P49, DOI 10.1590/S0036-46652012000100009
- Rocha BAM, 2017, INFECT DIS POVERTY, V6, DOI 10.1186/s40249-017-0328-9
- Sagulenko P, 2018, VIRUS EVOL, V4, DOI 10.1093/ve/vex042
- Secretaria da Saude-Governo do Estado de Sao Paulo, 2019, DISTR CAS DENG NOT C
- Secretaria de Saude Prefeitura de Sao Jose do Rio Preto, B DENG
- Secretaria de Vigilancia em Saude-Ministerio da Saude, 2020, MON CAS ARB URB TRAN
- Srikiatkhachorn A, 2011, CLIN INFECT DIS, V53, P563, DOI 10.1093/cid/cir451
- Tian HY, 2017, PLOS NEGLECT TROP D, V11, DOI 10.1371/journal.pntd.0005694
- Trifinopoulos J, 2016, NUCLEIC ACIDS RES, V44, pW232, DOI 10.1093/nar/gkw256
- Vicente CR, 2016, BMC INFECT DIS, V16, DOI 10.1186/s12879-016-1668-y
- Weaver SC, 2009, INFECT GENET EVOL, V9, P523, DOI 10.1016/j.meegid.2009.02.003
- Wick RR, 2019, GENOME BIOL, V20, DOI 10.1186/s13059-019-1727-y
- Williams M, 2014, AM J TROP MED HYG, V91, P611, DOI 10.4269/ajtmh.13-0600
- World Health Organization, 2021, DENG GUID DIAGN TREA
- World Health Organization, 2021, GLOB STRAT DENG PREV
- Yenamandra SP, 2021, SCI REP-UK, V11, DOI 10.1038/s41598-021-92783-y