Basophil Activation Test as a Biomarker for Taxanes Anaphylaxis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCAMPOS, Lucila De
dc.contributor.authorGIAVINA-BIANCHI, Pedro
dc.contributor.authorACHARYA, Shree
dc.contributor.authorLYNCH, Donna-Marie
dc.contributor.authorKALIL, Jorge
dc.contributor.authorCASTELLS, Mariana C.
dc.date.accessioned2023-10-30T14:24:56Z
dc.date.available2023-10-30T14:24:56Z
dc.date.issued2022
dc.description.abstractIntroductionTaxanes are widely used chemotherapy agents, and their administration, despite premedication, is associated with hypersensitivity reactions (HR) in up to 9% of patients, 1% of which are severe. The mechanisms of these reactions are not fully understood. Finding biomarkers for early diagnosis and better understanding the underlying mechanisms of these reactions are key to defining the best treatment strategy for patients.MethodsThe purpose of this study was to evaluate the effectiveness of the basophil activation test (BAT) to diagnose patients with anaphylactic reactions to taxanes. Patients with anaphylaxis to taxane compounds (n = 15) were assessed through clinical history, skin testing (when possible), and BAT. BAT was performed immediately before rapid drug desensitization or before skin testing using anti-CD123 conjugated (APC-Biolegend), anti-HLADR conjugated (FITC-Biolegend) to gate Basophils and anti-CD63 conjugated (PE-Biolegend), and anti-CD203c conjugated (BV-Biolegend) to assess CD203c and CD63 expression on basophils under taxane stimulation. BAT was also performed in eight healthy volunteers.ResultsBAT was positive for CD203c in eight out of 15 patients and for CD63 in four out of 15 patients and in two out of eight controls. The sensitivity for CD203c was 53%, the specificity was 87%, and the area under the curve was 0.66 (p = 0.19%). For CD63, these rates were 33%, 87%, and 0.6 (p = 0.4). In a subgroup analysis of patients with positive skin tests (11 patients), CD203c was positive in six patients (sensitivity of 54.5% and specificity of 87.5%), and CD63 was positive in five patients (sensitivity of 45% and specificity of 75%).ConclusionsBAT as a diagnostic tool for immediate hypersensitivity reactions to taxanes may be relevant in patients with selected phenotypes and endotypes, especially those with severe reactions or when the diagnosis cannot be established by the skin test. Increased expression of CD203c was more frequent than of CD63 in patients with positive results, and the sensitivity of this biomarker was higher in patient sub-group with positive skin tests, i.e., patients with IgE-mediated endotypes.eng
dc.description.indexPubMed
dc.description.indexWoS
dc.description.sponsorshipThis text acknowledges the contributions of all the colleagues from the Laboratory of the Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Womenamp;apos;s Hospital (BWH), Harvard Medical School, Boston, MA co. that sup
dc.description.sponsorshipDivision of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Womenamp;apos;s Hospital (BWH)
dc.description.sponsorshipHarvard Medical School, Boston, MA co.
dc.identifier.citationFRONTIERS IN ALLERGY, v.3, article ID 787749, 9p, 2022
dc.identifier.doi10.3389/falgy.2022.787749
dc.identifier.eissn2673-6101
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/55998
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SAeng
dc.relation.ispartofFrontiers in Allergy
dc.rightsopenAccesseng
dc.rights.holderCopyright FRONTIERS MEDIA SAeng
dc.subjectbasophil activation test (BAT)eng
dc.subjectimmediate hypersensitivity reactioneng
dc.subjecttaxanes agentseng
dc.subjectanaphylaxiseng
dc.subjectchemotherapyeng
dc.subject.otherhypersensitivity reactionseng
dc.subject.otherrapid desensitizationeng
dc.subject.othercomplement activationeng
dc.subject.otherantibody 97a6eng
dc.subject.othercremophor-eleng
dc.subject.otherchemotherapyeng
dc.subject.otherpaclitaxeleng
dc.subject.wosAllergyeng
dc.titleBasophil Activation Test as a Biomarker for Taxanes Anaphylaxiseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalACHARYA, Shree:Harvard Med Sch, Brigham & Womens Hosp BWH, Dept Med, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA
hcfmusp.author.externalLYNCH, Donna-Marie:Harvard Med Sch, Brigham & Womens Hosp BWH, Dept Med, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA
hcfmusp.author.externalCASTELLS, Mariana C.:Harvard Med Sch, Brigham & Womens Hosp BWH, Dept Med, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA
hcfmusp.contributor.author-fmusphcLUCILA DE CAMPOS
hcfmusp.contributor.author-fmusphcPEDRO FRANCISCO GIAVINA-BIANCHI JUNIOR
hcfmusp.contributor.author-fmusphcJORGE ELIAS KALIL FILHO
hcfmusp.description.articlenumber787749
hcfmusp.description.volume3
hcfmusp.origemWOS
hcfmusp.origem.pubmed35910859
hcfmusp.origem.wosWOS:001058648300001
hcfmusp.publisher.cityLAUSANNEeng
hcfmusp.publisher.countrySWITZERLANDeng
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