Assessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSILVA, Thiago Henrique Gomes da
dc.contributor.authorPAZETTI, Rogerio
dc.contributor.authorAOKI, Fabio Gava
dc.contributor.authorCARDOSO, Paulo Francisco Guerreiro
dc.contributor.authorVALENGA, Marcelo Henrique
dc.contributor.authorDEFFUNE, Elenice
dc.contributor.authorEVARISTO, Thaiane
dc.contributor.authorPEGO-FERNANDES, Paulo Manuel
dc.contributor.authorMORIYA, Henrique Takachi
dc.date.accessioned2015-02-06T19:10:54Z
dc.date.available2015-02-06T19:10:54Z
dc.date.issued2014
dc.description.abstractOBJECTIVES: Despite the recent success regarding the transplantation of tissue-engineered airways, the mechanical properties of these grafts are not well understood. Mechanical assessment of a tissue-engineered airway graft before implantation may be used in the future as a predictor of function. The aim of this preliminary work was to develop a noninvasive image-processing environment for the assessment of airway mechanics. METHOD: Decellularized, recellularized and normal tracheas (groups DECEL, RECEL, and CONTROL, respectively) immersed in Krebs-Henseleit solution were ventilated by a small-animal ventilator connected to a Fleisch pneumotachograph and two pressure transducers (differential and gauge). A camera connected to a stereomicroscope captured images of the pulsation of the trachea before instillation of saline solution and after instillation of Krebs-Henseleit solution, followed by instillation with Krebs-Henseleit with methacholine 0.1 M (protocols A, K and KMCh, respectively). The data were post-processed with computer software and statistical comparisons between groups and protocols were performed. RESULTS: There were statistically significant variations in the image measurements of the medial region of the trachea between the groups (two-way analysis of variance [ANOVA], p < 0.01) and of the proximal region between the groups and protocols (two-way ANOVA, p < 0.01). CONCLUSIONS: The technique developed in this study is an innovative method for performing a mechanical assessment of engineered tracheal grafts that will enable evaluation of the viscoelastic properties of neo-tracheas prior to transplantation.
dc.description.indexMEDLINE
dc.description.sponsorshipCNPq (National Counsel of Technological and Scientific Development)
dc.identifier.citationCLINICS, v.69, n.7, p.500-503, 2014
dc.identifier.doi10.6061/clinics/2014(07)11
dc.identifier.eissn1980-5322
dc.identifier.issn1807-5932
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/8380
dc.language.isoeng
dc.publisherHOSPITAL CLINICAS, UNIV SAO PAULO
dc.relation.ispartofClinics
dc.rightsopenAccess
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULO
dc.subjectInstrumentation
dc.subjectRespiratory Mechanics
dc.subjectTrachea
dc.subject.othertransplantation
dc.subject.wosMedicine, General & Internal
dc.titleAssessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalSILVA, Thiago Henrique Gomes da:Univ Sao Paulo, Biomed Engn Lab, Escola Politecn, Sao Paulo, Brazil
hcfmusp.author.externalAOKI, Fabio Gava:Univ Sao Paulo, Biomed Engn Lab, Escola Politecn, Sao Paulo, Brazil
hcfmusp.author.externalVALENGA, Marcelo Henrique:Univ Sao Paulo, Biomed Engn Lab, Escola Politecn, Sao Paulo, Brazil
hcfmusp.author.externalDEFFUNE, Elenice:Univ Estadual Paulista UNESP, Tissue Engn Lab, Botucatu, SP, Brazil
hcfmusp.author.externalEVARISTO, Thaiane:Univ Estadual Paulista UNESP, Tissue Engn Lab, Botucatu, SP, Brazil
hcfmusp.author.externalMORIYA, Henrique Takachi:Univ Sao Paulo, Biomed Engn Lab, Escola Politecn, Sao Paulo, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcROGERIO PAZETTI
hcfmusp.contributor.author-fmusphcPAULO FRANCISCO GUERREIRO CARDOSO
hcfmusp.contributor.author-fmusphcPAULO MANUEL PEGO FERNANDES
hcfmusp.description.beginpage500
hcfmusp.description.endpage503
hcfmusp.description.issue7
hcfmusp.description.volume69
hcfmusp.origemWOS
hcfmusp.origem.scopus2-s2.0-84904387621
hcfmusp.origem.wosWOS:000341379400011
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.scopus.lastupdate2024-05-10
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