Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLIMA, Gisele Negro
dc.contributor.authorMAGANHIN, Carla Cristina
dc.contributor.authorSIMOES, Ricardo Santos
dc.contributor.authorBARACAT, Maria Candida Pinheiro
dc.contributor.authorSASSO, Gisela Rodrigues da Silva
dc.contributor.authorFUCHS, Luiz Fernando Portugal
dc.contributor.authorSIMOES, Manuel de Jesus
dc.contributor.authorBARACAT, Edmund Chada
dc.contributor.authorSOARES JUNIOR, Jose Maria
dc.date.accessioned2015-08-14T15:39:00Z
dc.date.available2015-08-14T15:39:00Z
dc.date.issued2015
dc.description.abstractOBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 mu g/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip (R) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip (R) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis.
dc.description.indexMEDLINE
dc.description.sponsorshipFederal University of Sao Paulo
dc.description.sponsorshipFAPESP [2011/51581-8]
dc.identifier.citationCLINICS, v.70, n.2, p.144-151, 2015
dc.identifier.doi10.6061/clinics/2015(02)12
dc.identifier.eissn1980-5322
dc.identifier.issn1807-5932
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/9825
dc.language.isoeng
dc.publisherHOSPITAL CLINICAS, UNIV SAO PAULO
dc.relation.ispartofClinics
dc.rightsopenAccess
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULO
dc.subjectMelatonin
dc.subjectSteroidogenesis
dc.subjectGene Expression
dc.subjectOvary
dc.subjectRat
dc.subject.otherurinary 6-sulfatoxymelatonin excretion
dc.subject.othercyclic adenosine-3',5'-monophosphate
dc.subject.otherhyperandrogenic women
dc.subject.otheraromatase-activity
dc.subject.othercytochromes p450
dc.subject.otherbreast-cancer
dc.subject.otherestrous-cycle
dc.subject.otherfemale rats
dc.subject.othercells
dc.subject.otherpinealectomy
dc.subject.wosMedicine, General & Internal
dc.titleSteroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalLIMA, Gisele Negro:Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Sao Paulo, SP, Brazil
hcfmusp.author.externalMAGANHIN, Carla Cristina:Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Sao Paulo, SP, Brazil
hcfmusp.author.externalSASSO, Gisela Rodrigues da Silva:Univ Fed Sao Paulo, EPM UNIFESP, Paulista Sch Med, Dept Morphol & Genet, Sao Paulo, SP, Brazil
hcfmusp.author.externalSIMOES, Manuel de Jesus:Univ Fed Sao Paulo, EPM UNIFESP, Paulista Sch Med, Dept Morphol & Genet, Sao Paulo, SP, Brazil
hcfmusp.citation.scopus21
hcfmusp.contributor.author-fmusphcRICARDO DOS SANTOS SIMOES
hcfmusp.contributor.author-fmusphcMARIA CANDIDA PINHEIRO BARACAT REZENDE
hcfmusp.contributor.author-fmusphcLUIZ FERNANDO PORTUGAL FUCHS
hcfmusp.contributor.author-fmusphcEDMUND CHADA BARACAT
hcfmusp.contributor.author-fmusphcJOSE MARIA SOARES JUNIOR
hcfmusp.description.beginpage144
hcfmusp.description.endpage151
hcfmusp.description.issue2
hcfmusp.description.volume70
hcfmusp.origemWOS
hcfmusp.origem.pubmed25789524
hcfmusp.origem.scieloSCIELO:S1807-59322015000200144
hcfmusp.origem.scopus2-s2.0-84924931567
hcfmusp.origem.wosWOS:000351602700012
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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