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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | TEIXEIRA, Priscila Camillo | - |
dc.contributor.author | SANTOS, Ronaldo Honorato Barros | - |
dc.contributor.author | FIORELLI, Alfredo Inacio | - |
dc.contributor.author | BILATE, Angelina Morand Bianchi | - |
dc.contributor.author | BENVENUTI, Luiz Alberto | - |
dc.contributor.author | STOLF, Noedir Antonio | - |
dc.contributor.author | KALIL, Jorge | - |
dc.contributor.author | CUNHA-NETO, Edecio | - |
dc.date.accessioned | 2017-11-27T16:24:53Z | - |
dc.date.available | 2017-11-27T16:24:53Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | PLOS NEGLECTED TROPICAL DISEASES, v.5, n.6, article ID e1205, 9p, 2011 | - |
dc.identifier.issn | 1935-2727 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/22849 | - |
dc.description.abstract | Background: Chronic Chagas disease cardiomyopathy (CCC) is an inflammatory dilated cardiomyopathy with a worse prognosis than other cardiomyopathies. CCC occurs in 30 % of individuals infected with Trypanosoma cruzi, endemic in Latin America. Heart failure is associated with impaired energy metabolism, which may be correlated to contractile dysfunction. We thus analyzed the myocardial gene and protein expression, as well as activity, of key mitochondrial enzymes related to ATP production, in myocardial samples of end-stage CCC, idiopathic dilated (IDC) and ischemic (IC) cardiomyopathies. Methodology/Principal Findings: Myocardium homogenates from CCC (N = 5), IC (N = 5) and IDC (N = 5) patients, as well as from heart donors (N = 5) were analyzed for protein and mRNA expression of mitochondrial creatine kinase (CKMit) and muscular creatine kinase (CKM) and ATP synthase subunits aplha and beta by immunoblotting and by real-time RT-PCR. Total myocardial CK activity was also assessed. Protein levels of CKM and CK activity were reduced in all three cardiomyopathy groups. However, total CK activity, as well as ATP synthase alpha chain protein levels, were significantly lower in CCC samples than IC and IDC samples. CCC myocardium displayed selective reduction of protein levels and activity of enzymes crucial for maintaining cytoplasmic ATP levels. Conclusions/Significance: The selective impairment of the CK system may be associated to the loss of inotropic reserve observed in CCC. Reduction of ATP synthase alpha levels is consistent with a decrease in myocardial ATP generation through oxidative phosphorylation. Together, these results suggest that the energetic deficit is more intense in the myocardium of CCC patients than in the other tested dilated cardiomyopathies. | - |
dc.description.sponsorship | Brazilian Council for Scientific and Technological Development - CNPq | - |
dc.description.sponsorship | Sao Paulo State Research Funding Agency - FAPESP | - |
dc.language.iso | eng | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.relation.ispartof | Plos Neglected Tropical Diseases | - |
dc.rights | openAccess | - |
dc.subject.other | idiopathic dilated cardiomyopathy | - |
dc.subject.other | mitochondrial respiratory-chain | - |
dc.subject.other | trypanosoma-cruzi infection | - |
dc.subject.other | heart-failure | - |
dc.subject.other | ischemia-reperfusion | - |
dc.subject.other | energy-metabolism | - |
dc.subject.other | cardiac disease | - |
dc.subject.other | nitric-oxide | - |
dc.subject.other | myocarditis | - |
dc.subject.other | expression | - |
dc.title | Selective Decrease of Components of the Creatine Kinase System and ATP Synthase Complex in Chronic Chagas Disease Cardiomyopathy | - |
dc.type | article | - |
dc.rights.holder | Copyright PUBLIC LIBRARY SCIENCE | - |
dc.identifier.doi | 10.1371/journal.pntd.0001205 | - |
dc.identifier.pmid | 21738806 | - |
dc.subject.wos | Infectious Diseases | - |
dc.subject.wos | Parasitology | - |
dc.subject.wos | Tropical Medicine | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | BILATE, Angelina Morand Bianchi:Univ Sao Paulo, Sch Med, Immunol Lab, Inst Heart, Sao Paulo, Brazil | - |
hcfmusp.description.articlenumber | e1205 | - |
hcfmusp.description.issue | 6 | - |
hcfmusp.description.volume | 5 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000292139600041 | - |
hcfmusp.origem.id | 2-s2.0-79959843202 | - |
hcfmusp.publisher.city | SAN FRANCISCO | - |
hcfmusp.publisher.country | USA | - |
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dc.description.index | MEDLINE | - |
hcfmusp.citation.scopus | 24 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/11 Artigos e Materiais de Revistas Científicas - LIM/19 |
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