Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

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art_VESGUEIRO_Intragenomic_variation_in_the_second_internal_transcribed_spacer_2011.PDF (1.03 MB)
Citações na Scopus
25
Tipo de produção
article
Data de publicação
2011
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FUNDACO OSWALDO CRUZ
Autores
VESGUEIRO, Fabiana Tavares
DEMARI-SILVA, Bruna
SALLUM, Maria Anice Mureb
MARRELLI, Mauro Toledo
Citação
MEMORIAS DO INSTITUTO OSWALDO CRUZ, v.106, n.1, p.1-8, 2011
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Culex is the largest genus of Culicini and includes vectors of several arboviruses and filarial worms. Many species of Culex are morphologically similar, which makes their identification difficult, particularly when using female specimens. To aid evolutionary studies and species distinction, molecular techniques are often used. Sequences of the second internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) from 16 species of the genus Culex and one of Lutzia were used to assess their genomic variability and to verify their applicability in the phylogenetic analysis of the group. The distance matrix (uncorrected p-distance) that was obtained revealed intragenomic and intraspecific variation. Because of the intragenomic variability, we selected ITS2 copies for use in distance analyses based on their secondary structures. Neighbour-joining topology was obtained with an uncorrected p-distance. Despite the heterogeneity observed, individuals of the same species were grouped together and correlated with the current, morphology-based classification, thereby showing that ITS2 is an appropriate marker to be used in the taxonomy of Culex.
Palavras-chave
Diptera, Culex, ITS2, rDNA, phylogeny, concerted evolution
URI
https://observatorio.fm.usp.br/handle/OPI/23576
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Coleções
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/49