Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/276
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | ANTONANGELO, L. | - |
dc.contributor.author | VARGAS, F. S. | - |
dc.contributor.author | ACENCIO, M. M. P. | - |
dc.contributor.author | CORA, A. P. | - |
dc.contributor.author | TEIXEIRA, L. R. | - |
dc.contributor.author | GENOFRE, E. H. | - |
dc.contributor.author | SALES, R. K. B. | - |
dc.date.accessioned | 2013-07-30T14:35:06Z | - |
dc.date.available | 2013-07-30T14:35:06Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | CYTOPATHOLOGY, v.23, n.2, p.103-107, 2012 | - |
dc.identifier.issn | 0956-5507 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/276 | - |
dc.description.abstract | L. Antonangelo, F. S. Vargas, M. M. P. Acencio, A. P. Cora, L. R. Teixeira, E. H. Genofre and R. K. B. Sales Effect of temperature and storage time on cellular analysis of fresh pleural fluid samples Objective: Despite the methodological variability in preparation techniques for pleural fluid cytology, it is fundamental that the cells should be preserved, permitting adequate morphological classification. We evaluated numerical and morphological changes in pleural fluid specimens processed after storage at room temperature or under refrigeration. Methods: Aliquots of pleural fluid from 30 patients, collected in ethylenediaminetetraacetic acid-coated tubes and maintained at room temperature (21 degrees C) or refrigeration (4 degrees C) were evaluated after 2 and 6 hours and 1, 2, 3, 4, 7 and 14 days. Evaluation of cytomorphology and global and percentage counts of leucocytes, macrophages and mesothelial cells were included. Results: The samples had quantitative cellular variations from day 3 or 4 onwards, depending on the storage conditions. Morphological alterations occurred earlier in samples maintained at room temperature (day 2) than in those under refrigeration (day 4). Conclusions: This study confirms that storage time and temperature are potential pre-analytical causes of error in pleural fluid cytology. | - |
dc.description.sponsorship | Research Support Foundation for the State of Sao Paulo (FAPESP) | - |
dc.description.sponsorship | National Research Council (CNPq), Brazil | - |
dc.language.iso | eng | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.ispartof | Cytopathology | - |
dc.rights | restrictedAccess | - |
dc.subject | pleural fluid | - |
dc.subject | cytology | - |
dc.subject | mesothelial cells | - |
dc.subject | cytomorphology | - |
dc.subject | storage conditions | - |
dc.subject | error factors | - |
dc.subject.other | effusions | - |
dc.title | Effect of temperature and storage time on cellular analysis of fresh pleural fluid samples | - |
dc.type | article | - |
dc.rights.holder | Copyright WILEY-BLACKWELL | - |
dc.identifier.doi | 10.1111/j.1365-2303.2011.00863.x | - |
dc.identifier.pmid | 21418346 | - |
dc.subject.wos | Cell Biology | - |
dc.subject.wos | Pathology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.beginpage | 103 | - |
hcfmusp.description.endpage | 107 | - |
hcfmusp.description.issue | 2 | - |
hcfmusp.description.volume | 23 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84858633911 | - |
hcfmusp.origem.id | WOS:000301652800006 | - |
hcfmusp.publisher.city | MALDEN | - |
hcfmusp.publisher.country | USA | - |
hcfmusp.relation.reference | Antonangelo L, 2007, CLINICS, V62, P585 | - |
hcfmusp.relation.reference | Conner BD, 2003, CHEST, V123, P1181, DOI 10.1378/chest.123.4.1181 | - |
hcfmusp.relation.reference | GUZMAN J, 1993, ACTA CYTOL, V37, P267 | - |
hcfmusp.relation.reference | GUZMAN J, 1992, ANAL QUANT CYTOL, V14, P203 | - |
hcfmusp.relation.reference | LIGHT RW, 1972, ANN INTERN MED, V77, P507 | - |
hcfmusp.relation.reference | Lopes Cardozo P, 1979, ATLAS CLIN CYTOLOGY | - |
hcfmusp.relation.reference | Manosca F, 2007, DIAGN CYTOPATHOL, V35, P6, DOI 10.1002/dc.20587 | - |
hcfmusp.relation.reference | Maskell NA, 2003, THORAX S2, V58, pii8 | - |
dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | CNPq | - |
hcfmusp.remissive.sponsorship | FAPESP | - |
hcfmusp.lim.ref | 2012 | - |
hcfmusp.citation.scopus | 17 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - HC/IOT Artigos e Materiais de Revistas Científicas - LIM/03 Artigos e Materiais de Revistas Científicas - LIM/09 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_ANTONANGELO_Effect_of_temperature_and_storage_time_on_cellular_2012_eng.pdf Restricted Access | publishedVersion (English) | 120.45 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.