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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | BATISSOCO, Ana C. | |
dc.contributor.author | SALAZAR-SILVA, Rodrigo | |
dc.contributor.author | OITICICA, Jeanne | |
dc.contributor.author | BENTO, Ricardo F. | |
dc.contributor.author | MINGRONI-NETTO, Regina C. | |
dc.contributor.author | HADDAD, Luciana A. | |
dc.date.accessioned | 2019-01-17T13:34:12Z | - |
dc.date.available | 2019-01-17T13:34:12Z | - |
dc.date.issued | 2018 | |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, n.9, article ID 2535, 20p, 2018 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29979 | - |
dc.description.abstract | GJB2 mutations are the leading cause of non-syndromic inherited hearing loss. GJB2 encodes connexin-26 (CX26), which is a connexin (CX) family protein expressed in cochlea, skin, liver, and brain, displaying short cytoplasmic N-termini and C-termini. We searched for CX26 C-terminus binding partners by affinity capture and identified 12 unique proteins associated with cell junctions or cytoskeleton (CGN, DAAM1, FLNB, GAPDH, HOMER2, MAP7, MAPRE2 (EB2), JUP, PTK2B, RAI14, TJP1, and VCL) by using mass spectrometry. We show that, similar to other CX family members, CX26 co-fractionates with TJP1, VCL, and EB2 (EB1 paralogue) as well as the membrane-associated protein ASS1. The adaptor protein CGN (cingulin) co-immuno-precipitates with CX26, ASS1, and TJP1. In addition, CGN co-immunoprecipitation with CX30, CX31, and CX43 indicates that CX association is independent on the CX C-terminus length or sequence. CX26, CGN, FLNB, and DAMM1 were shown to distribute to the organ of Corti and hepatocyte plasma membrane. In the mouse liver, CX26 and TJP1 co-localized at the plasma membrane. In conclusion, CX26 associates with components of other membrane junctions that integrate with the cytoskeleton. | eng |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)-CEPID-Centro de Pesquisa sobre o Genoma Humano e Celulas-Tronco [13/08028-1] | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/CNPq [140009/2007-8] | |
dc.description.sponsorship | FAPESP [12/50154-1] | |
dc.language.iso | eng | |
dc.publisher | MDPI | eng |
dc.relation.ispartof | International Journal of Molecular Sciences | |
dc.rights | openAccess | eng |
dc.subject | connexin | eng |
dc.subject | connexin 26 | eng |
dc.subject | GJB2 gene | eng |
dc.subject | deafness | eng |
dc.subject | protein-protein interaction | eng |
dc.subject | TJP1 | eng |
dc.subject | CGN | eng |
dc.subject | FLNB | eng |
dc.subject | DAAM1 | eng |
dc.subject | organ of Corti | eng |
dc.subject.other | f-box protein | eng |
dc.subject.other | zonula occludens-1 | eng |
dc.subject.other | tight-junction | eng |
dc.subject.other | gap-junctions | eng |
dc.subject.other | cardiac myocytes | eng |
dc.subject.other | directly binds | eng |
dc.subject.other | mouse-brain | eng |
dc.subject.other | pdz domain | eng |
dc.subject.other | zo-1 | eng |
dc.subject.other | interacts | eng |
dc.title | A Cell Junctional Protein Network Associated with Connexin-26 | eng |
dc.type | article | eng |
dc.rights.holder | Copyright MDPI | eng |
dc.identifier.doi | 10.3390/ijms19092535 | |
dc.identifier.pmid | 30150563 | |
dc.subject.wos | Biochemistry & Molecular Biology | eng |
dc.subject.wos | Chemistry, Multidisciplinary | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | SALAZAR-SILVA, Rodrigo:Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, Human Genome & Stem Cell Res Ctr, BR-05508090 Sao Paulo, Brazil | |
hcfmusp.author.external | MINGRONI-NETTO, Regina C.:Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, Human Genome & Stem Cell Res Ctr, BR-05508090 Sao Paulo, Brazil | |
hcfmusp.author.external | HADDAD, Luciana A.:Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, Human Genome & Stem Cell Res Ctr, BR-05508090 Sao Paulo, Brazil | |
hcfmusp.description.articlenumber | 2535 | |
hcfmusp.description.issue | 9 | |
hcfmusp.description.volume | 19 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000449988100071 | |
hcfmusp.origem.id | 2-s2.0-85052502387 | |
hcfmusp.publisher.city | BASEL | eng |
hcfmusp.publisher.country | SWITZERLAND | eng |
hcfmusp.relation.reference | Agullo-Pascual E, 2014, CARDIOVASC RES, V104, P371, DOI 10.1093/cvr/cvu195 | eng |
hcfmusp.relation.reference | Ajima R, 2007, BIOCHEM BIOPH RES CO, V356, P851, DOI 10.1016/j.bbrc.2007.03.060 | eng |
hcfmusp.relation.reference | Angeli S, 2012, ANAT REC, V295, P1812, DOI 10.1002/ar.22579 | eng |
hcfmusp.relation.reference | Azaiez H, 2015, PLOS GENET, V11, DOI 10.1371/journal.pgen.1005137 | eng |
hcfmusp.relation.reference | Baker JA, 2017, BMC BIOL, V15, DOI 10.1186/s12915-017-0404-4 | eng |
hcfmusp.relation.reference | Beltramello M, 2005, NAT CELL BIOL, V7, P63, DOI 10.1038/ncb1205 | eng |
hcfmusp.relation.reference | Chkourko HS, 2012, HEART RHYTHM, V9, P1133, DOI 10.1016/j.hrthm.2012.03.003 | eng |
hcfmusp.relation.reference | Cordenonsi M, 1999, J CELL BIOL, V147, P1569, DOI 10.1083/jcb.147.7.1569 | eng |
hcfmusp.relation.reference | Dbouk HA, 2009, CELL COMMUN SIGNAL, V7, DOI 10.1186/1478-811X-7-4 | eng |
hcfmusp.relation.reference | del Castillo FJ, 2010, HUM MOL GENET, V19, P262, DOI 10.1093/hmg/ddp490 | eng |
hcfmusp.relation.reference | Derangeon M, 2009, BBA-BIOMEMBRANES, V1788, P768, DOI 10.1016/j.bbamem.2008.10.023 | eng |
hcfmusp.relation.reference | Donahue HJ, 2018, NAT REV RHEUMATOL, V14, P42, DOI 10.1038/nrrheum.2017.204 | eng |
hcfmusp.relation.reference | Dror AA, 2009, ANNU REV GENET, V43, P411, DOI 10.1146/annurev-genet-102108-134135 | eng |
hcfmusp.relation.reference | ExPASy, SIB BIOINF RES PORT | eng |
hcfmusp.relation.reference | Flam BR, 2001, NITRIC OXIDE-BIOL CH, V5, P187, DOI 10.1006/niox.2001.0340 | eng |
hcfmusp.relation.reference | Flores CE, 2008, P NATL ACAD SCI USA, V105, P12545, DOI 10.1073/pnas.0804793105 | eng |
hcfmusp.relation.reference | Fowler SL, 2013, J PROTEOME RES, V12, P2597, DOI 10.1021/pr301166p | eng |
hcfmusp.relation.reference | Furuse M, 1998, J CELL BIOL, V141, P1539, DOI 10.1083/jcb.141.7.1539 | eng |
hcfmusp.relation.reference | Gasparini P, 1997, EUR J HUM GENET, V5, P83 | eng |
hcfmusp.relation.reference | Giepmans BNG, 1998, CURR BIOL, V8, P931, DOI 10.1016/S0960-9822(07)00375-2 | eng |
hcfmusp.relation.reference | Haines Ricci J, 2011, Int J Biochem Mol Biol, V2, P8 | eng |
hcfmusp.relation.reference | Havlis J, 2003, ANAL CHEM, V75, P1300, DOI 10.1021/ac026136s | eng |
hcfmusp.relation.reference | Henzl MT, 2004, HEARING RES, V191, P101, DOI 10.1016/j.heares.2004.01.005 | eng |
hcfmusp.relation.reference | Herve JC, 2012, BBA-BIOMEMBRANES, V1818, P1844, DOI 10.1016/j.bbamem.2011.12.009 | eng |
hcfmusp.relation.reference | Johnson SL, 2017, J NEUROSCI, V37, P258, DOI [10.1523/JNEUROSCI.2251-16.2017, 10.1523/JNEUROSCI.2251-16.2016] | eng |
hcfmusp.relation.reference | Karademir LB, 2016, BIOCHEM J, V473, P1391, DOI 10.1042/BCJ20160200 | eng |
hcfmusp.relation.reference | Kausalya PJ, 2001, FEBS LETT, V505, P92, DOI 10.1016/S0014-5793(01)02786-7 | eng |
hcfmusp.relation.reference | Keller A, 2002, ANAL CHEM, V74, P5383, DOI 10.1021/ac025747h | eng |
hcfmusp.relation.reference | Kelsell DP, 1997, NATURE, V387, P80, DOI 10.1038/387080a0 | eng |
hcfmusp.relation.reference | Kojima T, 1999, BIOCHEM BIOPH RES CO, V266, P222, DOI 10.1006/bbrc.1999.1778 | eng |
hcfmusp.relation.reference | Kojima T, 2001, EXP CELL RES, V263, P193, DOI 10.1006/excr.2000.5103 | eng |
hcfmusp.relation.reference | Komarova Y, 2005, MOL BIOL CELL, V16, P5334, DOI 10.1091/mbc.E05-07-0614 | eng |
hcfmusp.relation.reference | Laird DW, 2010, TRENDS CELL BIOL, V20, P92, DOI 10.1016/j.tcb.2009.11.001 | eng |
hcfmusp.relation.reference | Leithe E, 2018, BBA-BIOMEMBRANES, V1860, P48, DOI 10.1016/j.bbamem.2017.05.008 | eng |
hcfmusp.relation.reference | Li XB, 2004, EUR J NEUROSCI, V19, P2132, DOI 10.1111/j.1460-9568.2004.03283.x | eng |
hcfmusp.relation.reference | Maeda S, 2009, NATURE, V458, P597, DOI 10.1038/nature07869 | eng |
hcfmusp.relation.reference | MASSON D, 1995, J CELL BIOL, V131, P1015, DOI 10.1083/jcb.131.4.1015 | eng |
hcfmusp.relation.reference | Metzger T, 2012, NATURE, V484, P120, DOI 10.1038/nature10914 | eng |
hcfmusp.relation.reference | Nelson RF, 2007, J NEUROSCI, V27, P5163, DOI 10.1523/JNEUROSCI.0206-07.2007 | eng |
hcfmusp.relation.reference | Nielsen PA, 2003, MOL BIOL CELL, V14, P2470, DOI 10.1091/mbc.E02-10-0637 | eng |
hcfmusp.relation.reference | Nusrat A, 2000, J BIOL CHEM, V275, P29816, DOI 10.1074/jbc.M002450200 | eng |
hcfmusp.relation.reference | Peixoto PM, 2009, BIOCHEM BIOPH RES CO, V390, P38, DOI 10.1016/j.bbrc.2009.09.054 | eng |
hcfmusp.relation.reference | Penes MC, 2005, EUR J NEUROSCI, V22, P404, DOI 10.1111/j.1460-9568.2005.04225.x | eng |
hcfmusp.relation.reference | Peng YF, 2000, GENES CELLS, V5, P1001, DOI 10.1046/j.1365-2443.2000.00381.x | eng |
hcfmusp.relation.reference | Riazuddin S, 2006, AM J HUM GENET, V79, P1040, DOI 10.1086/510022 | eng |
hcfmusp.relation.reference | Rodriguez-Sinovas A, 2006, CIRC RES, V99, P93, DOI 10.1161/01.RES.0000230315.56904.de | eng |
hcfmusp.relation.reference | Sajan SA, 2007, GENETICS, V177, P631, DOI 10.1534/genetics.107.078584 | eng |
hcfmusp.relation.reference | Schubert AL, 2002, BIOCHEMISTRY-US, V41, P5754, DOI 10.1021/bi0121656 | eng |
hcfmusp.relation.reference | Shaw RM, 2007, CELL, V128, P547, DOI 10.1016/j.cell.2006.12.037 | eng |
hcfmusp.relation.reference | Shearer AE, 1993, GENEREVIEWS | eng |
hcfmusp.relation.reference | Shen J, 2015, DATABASE-OXFORD, DOI 10.1093/database/bav071 | eng |
hcfmusp.relation.reference | Shevchenko A, 1996, ANAL CHEM, V68, P850, DOI 10.1021/ac950914h | eng |
hcfmusp.relation.reference | Shiraishi-Yamaguchi Y, 2009, BMC NEUROSCI, V10, DOI 10.1186/1471-2202-10-25 | eng |
hcfmusp.relation.reference | Singh D, 2005, J BIOL CHEM, V280, P30416, DOI 10.1074/jbc.M506799200 | eng |
hcfmusp.relation.reference | Smith RJ, 1993, GENEREVIEWS | eng |
hcfmusp.relation.reference | Sorgen PL, 2018, INT J MOL SCI, V19, DOI 10.3390/ijms19051428 | eng |
hcfmusp.relation.reference | Stains JP, 2016, SEMIN CELL DEV BIOL, V50, P31, DOI 10.1016/j.semcdb.2015.12.017 | eng |
hcfmusp.relation.reference | STRING, FUNCT PROT ASS NETW | eng |
hcfmusp.relation.reference | Thevenin AF, 2013, PHYSIOLOGY, V28, P93, DOI 10.1152/physiol.00038.2012 | eng |
hcfmusp.relation.reference | Toyofuku T, 1998, J BIOL CHEM, V273, P12725, DOI 10.1074/jbc.273.21.12725 | eng |
hcfmusp.relation.reference | Wang XG, 1998, J MEMBRANE BIOL, V162, P169, DOI 10.1007/s002329900354 | eng |
hcfmusp.relation.reference | Wangemann P, 2002, AUDIOL NEURO-OTOL, V7, P199, DOI 10.1159/000063736 | eng |
hcfmusp.relation.reference | Xiao DZ, 2014, INT J BIOCHEM CELL B, V55, P288, DOI 10.1016/j.biocel.2014.09.021 | eng |
hcfmusp.relation.reference | Xu X, 2006, DEVELOPMENT, V133, P3629, DOI 10.1242/dev.02543 | eng |
hcfmusp.relation.reference | Yue JP, 2014, DEV CELL, V31, P572, DOI 10.1016/j.devcel.2014.10.025 | eng |
hcfmusp.relation.reference | Yum SW, 2007, AM J PHYSIOL-CELL PH, V293, pC1032, DOI 10.1152/ajpcell.00011.2007 | eng |
hcfmusp.relation.reference | Zemljic-Harpf AE, 2014, J CELL SCI, V127, P1104, DOI 10.1242/jcs.143743 | eng |
hcfmusp.relation.reference | Zhang YP, 2005, P NATL ACAD SCI USA, V102, P15201, DOI 10.1073/pnas.0501859102 | eng |
hcfmusp.relation.reference | Zhu Y, 2015, SCI REP-UK, V5, DOI 10.1038/srep15647 | eng |
hcfmusp.relation.reference | Ziegler WH, 2008, BIOCHEM SOC T, V36, P235, DOI 10.1042/BST0360235 | eng |
dc.description.index | MEDLINE | eng |
hcfmusp.citation.scopus | 12 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
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