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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | TANAKA, Leonardo Yuji | - |
dc.contributor.author | ARAUJO, Haniel Alves | - |
dc.contributor.author | CSORDAS, Andre Alcantara | - |
dc.contributor.author | HIRONAKA, Gustavo Ken | - |
dc.contributor.author | TAKIMURA, Celso Kiyochi | - |
dc.contributor.author | LAURINDO, Francisco Rafael Martins | - |
dc.date.accessioned | 2013-10-11T21:31:47Z | - |
dc.date.available | 2013-10-11T21:31:47Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | FASEB JOURNAL, v.26, 2012 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/3145 | - |
dc.description.abstract | Endoplasmic reticulum(ER) redox chaperone protein disulfide isomerase(PDI) regulates vascular/phagocytic NADPH oxidase and supports cell migration. We investigated the role of PDI during vascular repair after injury(AI) induced by balloon in rabbit iliac artery. There was marked increase of PDI mRNA and protein(5–10-fold) at 4, 7 and 14 days AI vs. intact control(CT). PDI immunostaining was greater in intima = neo-endothelium > media. Increased cell-surface PDI was also evident. ER stress-related KDEL chaperones also increased with similar time-course AI. PDI siRNA(siPDI) transfection in cultured vessel rings collected 14 days AI enhanced KDEL expression vs. scrambled siRNA(siScr) (siScr 2.1±0.9 vs. siPDI 5.0±2.3-fold vs. CT, p<0.05), apoptosis (siScr 4.6±0.2 vs. siPDI 6.2±0.9 %TUNEL + nuclei, p<0.05) and proliferation marker PCNA (siScr 1.2±0.3 vs. siPDI 4.0 ±0.2 AU, p<0.05), and decreased differentiation marker calponin-C (siScr 0.52±0.04 vs. siPDI 0.36 ±0.04 AU, p<0.05). siPDI in CT rings did not alter such variables. PCR array analysis showed analogous pattern of mRNA changes. Also, siPDI 14 days AI upregulated Nox1 and downregulated Nox4 NADPH oxidase, while siPDI attenuated oxidant production (in situ hydroethidine) only in CT vessels. Thus, strongly-overexpressed PDI 14 days AI protects against apoptosis and ER stress and sustains VSMC differentiation. | - |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Instituto Nacional de Ciência e Tecnologia (INCT) de Processos Redox em Biomedicina (Redoxoma) | - |
dc.language.iso | eng | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.relation.ispartof | Faseb Journal | - |
dc.rights | restrictedAccess | - |
dc.title | Role of Protein Disulfide Isomerase during vascular repair after injury | - |
dc.type | conferenceObject | - |
dc.rights.holder | Copyright FEDERATION AMER SOC EXP BIOL | - |
dc.description.conferencedate | APR 21-25, 2012 | - |
dc.description.conferencelocal | San Diego - CA, EUA | - |
dc.description.conferencename | Experimental Biology Meeting | - |
dc.subject.wos | Biochemistry & Molecular Biology | - |
dc.subject.wos | Biology | - |
dc.subject.wos | Cell Biology | - |
dc.type.category | meeting abstract | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.volume | 26 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000310711304460 | - |
hcfmusp.publisher.city | BETHESDA | - |
hcfmusp.publisher.country | USA | - |
dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | CNPq | - |
hcfmusp.remissive.sponsorship | FAPESP | - |
hcfmusp.remissive.sponsorship | INCTs | - |
Appears in Collections: | Comunicações em Eventos - HC/InCor Comunicações em Eventos - LIM/19 |
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