Usefulness of aqueous and vitreous humor analysis in infectious uveitis

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art_SANTOSGH_Usefulness_of_aqueous_and_vitreous_humor_analysis_in_2020.PDF (375.01 KB)
Citações na Scopus
12
Tipo de produção
article
Data de publicação
2020
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
HOSPITAL CLINICAS, UNIV SAO PAULO
Autores
SANTOSGH, Helen Nazareth Veloso dos
FERRACIOLI-ODA, Eduardo
OTANI, Camila Sayuri Vicentini
TANAKA, Tatiana
SILVA, Luciane de Carvalho Sarahyba da
LOPES, Guilherme de Oliveira
HIRATA, Carlos Eduardo
YAMAMOTO, Joyce Hisae
Citação
CLINICS, v.75, article ID e1498, 6p, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.
Palavras-chave
Polymerase Chain Reaction, Infectious Disease, Uveitis, Aqueous Humor, Vitreous Humor
URI
https://observatorio.fm.usp.br/handle/OPI/35614
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - ODS/03