LIM/36 - Laboratório de Pediatria Clínica
URI Permanente desta comunidade
O Laboratório de Pediatria Clínica é ligado ao Departamento de Pediatria da Faculdade de Medicina da Universidade de São Paulo (FMUSP).
Linhas de pesquisa: marcadores celulares e moleculares de infecções pediátricas; investigação molecular de doenças genéticas; investigação laboratorial de imunodeficiência; alergia alimentar na infância; investigação laboratorial de doenças endocrinológicas pediátricas; fisiopatologia e diagnóstico de afecções nefrológicas em crianças; fisiopatologia e diagnóstico de doenças autoimunes; investigação laboratorial de neoplasias pediátricas; investigação clínica e laboratorial do binômio mãe-filho e genômica pediátrica.
Site oficial: http://limhc.fm.usp.br/portal/lim36-laboratorio-de-pediatria-clinica/
Linhas de pesquisa: marcadores celulares e moleculares de infecções pediátricas; investigação molecular de doenças genéticas; investigação laboratorial de imunodeficiência; alergia alimentar na infância; investigação laboratorial de doenças endocrinológicas pediátricas; fisiopatologia e diagnóstico de afecções nefrológicas em crianças; fisiopatologia e diagnóstico de doenças autoimunes; investigação laboratorial de neoplasias pediátricas; investigação clínica e laboratorial do binômio mãe-filho e genômica pediátrica.
Site oficial: http://limhc.fm.usp.br/portal/lim36-laboratorio-de-pediatria-clinica/
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- LIM/36 - Laboratório de Pediatria Clínica
- LIM/36 - Laboratório de Pediatria Clínica
- LIM/36 - Laboratório de Pediatria Clínica
Submissões Recentes
Data-driven, cross-disciplinary collaboration: lessons learned at the largest academic health center in Latin America during the COVID-19 pandemic
(2024) RITTO, Ana Paula; ARAUJO, Adriana Ladeira de; CARVALHO, Carlos Roberto Ribeiro de; SOUZA, Heraldo Possolo De; FAVARETTO, Patricia Manga e Silva; SABOYA, Vivian Renata Boldrim; GARCIA, Michelle Louvaes; KULIKOWSKI, Leslie Domenici; KALLAS, Esper Georges; PEREIRA, Antonio Jose Rodrigues; COBELLO JUNIOR, Vilson; SILVA, Katia Regina; ABDALLA, Eidi Raquel Franco; SEGURADO, Aluisio Augusto Cotrim; SABINO, Ester Cerdeira; RIBEIRO JUNIOR, Ulysses; FRANCISCO, Rossana Pulcineli Vieira; MIETHKE-MORAIS, Anna; LEVIN, Anna Sara Shafferman; SAWAMURA, Marcio Valente Yamada; FERREIRA, Juliana Carvalho; SILVA, Clovis Artur; MAUAD, Thais; GOUVEIA, Nelson da Cruz; LETAIF, Leila Suemi Harima; BEGO, Marco Antonio; BATTISTELLA, Linamara Rizzo; DUARTE, Alberto Jose da Silva; SEELAENDER, Marilia Cerqueira Leite; MARCHINI, Julio; FORLENZA, Orestes Vicente; ROCHA, Vanderson Geraldo; MENDES-CORREA, Maria Cassia; COSTA, Silvia Figueiredo; CERRI, Giovanni Guido; BONFA, Eloisa Silva Dutra de Oliveira; CHAMMAS, Roger; BARROS FILHO, Tarcisio Eloy Pessoa de; BUSATTO FILHO, Geraldo
Introduction The COVID-19 pandemic has prompted global research efforts to reduce infection impact, highlighting the potential of cross-disciplinary collaboration to enhance research quality and efficiency.Methods At the FMUSP-HC academic health system, we implemented innovative flow management routines for collecting, organizing and analyzing demographic data, COVID-related data and biological materials from over 4,500 patients with confirmed SARS-CoV-2 infection hospitalized from 2020 to 2022. This strategy was mainly planned in three areas: organizing a database with data from the hospitalizations; setting-up a multidisciplinary taskforce to conduct follow-up assessments after discharge; and organizing a biobank. Additionally, a COVID-19 curated collection was created within the institutional digital library of academic papers to map the research output.Results Over the course of the experience, the possible benefits and challenges of this type of research support approach were identified and discussed, leading to a set of recommended strategies to enhance collaboration within the research institution. Demographic and clinical data from COVID-19 hospitalizations were compiled in a database including adults and a minority of children and adolescents with laboratory confirmed COVID-19, covering 2020-2022, with approximately 350 fields per patient. To date, this database has been used in 16 published studies. Additionally, we assessed 700 adults 6 to 11 months after hospitalization through comprehensive, multidisciplinary in-person evaluations; this database, comprising around 2000 fields per subject, was used in 15 publications. Furthermore, thousands of blood samples collected during the acute phase and follow-up assessments remain stored for future investigations. To date, more than 3,700 aliquots have been used in ongoing research investigating various aspects of COVID-19. Lastly, the mapping of the overall research output revealed that between 2020 and 2022 our academic system produced 1,394 scientific articles on COVID-19.Discussion Research is a crucial component of an effective epidemic response, and the preparation process should include a well-defined plan for organizing and sharing resources. The initiatives described in the present paper were successful in our aim to foster large-scale research in our institution. Although a single model may not be appropriate for all contexts, cross-disciplinary collaboration and open data sharing should make health research systems more efficient to generate the best evidence.
THE INFLUENCE OF ENVIRONMENTAL FACTORS RELATING TO JUVENILE DERMATOMYOSITIS′S COURSE AND REFRACTORINESS TO TREATMENT
(2023) VALOES, Clarissa C. M.; ARABI, Tamima M. A.; BRAGA, Alfesio L. F.; FARHAT, Sylvia C. L.; SALLUM, Adriana M. E.
NON-MYELOABLATIVE HAPLOIDENTICAL BMT WITH PTCY FOR CHILDREN AND ADULTS WITH SICKLE CELL DISEASES: THE BRAZILIAN EXPERIENCE
(2023) BONFIM, Carmem; SIMOES, Belinda; GOUVEIA, Roseane V.; SILVA, Roberto Luiz; LEITE, Lauro Augusto C.; FERNANDES, Juliana Folloni; GARCIA, Julia Lopes; ZECCHIN, Victor Gottardello; LEE, Maria Lucia; KUWAHARA, Cilmara; GOMES, Alessandra Araujo; RODRIGUES, Celso Arrais; NICHELE, Samantha; LOTH, Gisele; GINANI, Valeria Cortze; FELICIANO, Joao Vitor; LIMA, Alberto Cardoso Martins; DARRIGO JR., Luiz Guilherme; FUENTE, Josu De La; KASSIM, Adetola; SEBER, Adriana
Pineal region high grade neuroepithelial tumors with NTRK fusions belonging to the novel methylation class ""diffuse high grade glioma, IDH-wildtype, subtype E"" (HGG_E)-A distinct clinicopathological and molecular presentation
(2023) COSTA, F. D'Almeida; CASTRO, J. V. Alves de; KULIKOWSKI, L. Domenici; WOLFF, B.; GREGIANIN, L. J.; NETO, C. Scapulatempo; KOTIDIS, C.; DALAHMAH, O. Al; CANOLL, P.; BRUCE, J.; ALDAPE, K.; ABDULLAEV, Z.; NASRALLAH, M.; ZANAZZI, G.
Buccal cell whole exome sequencing improves the diagnostic yield in a Cornelia de Lange Syndrome Brazilian cohort
(2024) NUNES, Beatriz Carvalho; FAVILLA, Bianca Pereira; VILELLA, Thaina; PINHEIRO, Isabel; AOI, Haromi; SEYAMA, Rie; MATSUMOTO, Naomichi; BELLUCCO, Fernanda Teixeira; KIM, Chong; MELARAGNO, Maria Isabel
Investigation of copy number variations as possible genetic modifiers in patients with the 22q11.2 deletion syndrome
(2023) NUNES, Beatriz Carvalho; ZAMARIOLLI, Malu; DANTAS, Anelisa Gollo; SOARES, Diogo Cordeiro Queiroz; KIM, Chong Ae; MELARAGNO, Maria Isabel
A 43 years-old patient with Cornelia de Lange Syndrome with NIPBL gene mutation and a mild phenotype
(2024) VILELLA, Thaina; NUNES, Beatriz Carvalho; PINHEIRO, Isabel; AOI, Haromi; MATSUMOTO, Naomichi; KIM, Chong; MELARAGNO, Maria Isabel
Are the Levels of Cytokines Good Biomarkers for Smoldering Disease Activity in Childhood-Takayasu Arteritis?
(2023) CLEMENTE, Gleice; TERRERI, Maria Teresa; GUALANO, Bruno; SILVA, Clovis; SOUZA, Alexandre Wagner De
Establishment of oral microbiome in very low birth weight infants during the first weeks of life and the impact of oral diet implementation
(2023) VANZELE, Pedro A. R.; SPARVOLI, Luiz Gustavo; CAMARGO, Patricia P. de; TRAGANTE, Carla R.; BEOZZO, Glenda P. N. S.; KREBS, Vera L. J.; CORTEZ, Ramon V.; TADDEI, Carla R.
Very low birth weight (VLBW) infants, mostly preterm, have many barriers to feeding directly from the mother's breast, and need to be fed alternatively. Feeding is a major influencer in oral microbial colonization, and this colonization in early life is crucial for the promotion of human health. Therefore, this research aimed to observe the establishment of oral microbiome in VLBW infants during their first month of life through hospitalization, and to verify the impact caused by the implementation of oral diet on the colonization of these newborns. We included 23 newborns followed during hospitalization and analyzed saliva samples collected weekly, using 16S rRNA gene sequencing. We observed a significant decrease in richness and diversity and an increase in dominance over time (q-value < 0.05). The oral microbiome is highly dynamic during the first weeks of life, and beta diversity suggests a microbial succession in early life. The introduction of oral diet does not change the community structure, but affects the abundance, especially of Streptococcus. Our results indicate that although time is related to significant changes in the oral microbial profile, oral feeding benefits genera that will remain colonizers throughout the host's life.
A randomized, double-blind, non-inferiority trial comparing the immunogenicity and safety of two seasonal inactivated influenza vaccines in adults
(2023) VANNI, Tazio; SALOMAO, Maria da Grada; VISCONDI, Juliana Yukari Kodaira; BRAGA, Patricia Emilia; SILVA, Anderson da; PIORELLI, Roberta de Oliveira; SANTOS, Joane do Prado; GATTAS, Vera Lucia; LUCCHESI, Maria Beatriz Bastos; OLIVEIRA, Mayra Martho Moura de; KOIKE, Marcelo Eiji; CAMPOS, Lucia M. A.; COELHO, Eduardo B.; WECKX, Lily Yin; LARA, Amanda Nazareth; PAIVA, Terezinha M.; TIMENETSKY, Maria do Carmo S. T.; PRECIOSO, Alexander Roberto
Background: To enhance the production and availability of influenza vaccines in different regions of the world is paramount to mitigate the global burden of this disease. Instituto Butantan developed and man-ufactured an embryonated egg-based inactivated split-virion trivalent seasonal influenza vaccine as part of a technology transfer partnership with Sanofi Pasteur.Methods: This is a phase IV, randomized, double-blind, active-controlled, multicenter clinical trial includ-ing adults 18-60 and > 60 years recruited during the 2019 southern hemisphere influenza season. Subjects were randomized 1:1 to receive either the Sanofi Pasteur Trivalent Seasonal Influenza Vaccine (SP-TIV) or Instituto Butantan Trivalent Seasonal Influenza Vaccine (IB-TIV). Hemagglutinin inhibition antibody titers were assessed pre-vaccination and 21 days post-vaccination.Results: 624 participants were randomized and vaccinated. In both intention-to-treat and per-protocol analysis, non-inferiority of the SP-TIV versus IB-TIV was demonstrated for the three influenza strains. In the per-protocol analysis, the SP-GMT/IB-GMT ratios for H1N1, H3N2, and B were 0.9 (95%CI, 0.7- 1.1), 1.2 (95%CI, 1.0-1.4), and 1.1 (95%CI, 0.9-1.3), respectively. Across vaccination groups, the most com-mon adverse reactions (AR) were limited to the injection-site, including pain and tenderness. The major-ity of the ARs were graded 1 and/or 2 and lasted less than one day. No serious adverse reaction was observed.Conclusion: This study demonstrated the non-inferiority of the immunogenicity of a single-dose of Instituto Butantan versus a single dose of the Sanofi Pasteur Seasonal Trivalent Influenza Vaccine in adults. Both vaccines were well tolerated and presented similar safety profiles.(c) 2023 Elsevier Ltd. All rights reserved.