https://observatorio.fm.usp.br/handle/OPI/42105
Title: | Use of paclitaxel carried in lipid nanoparticles to treat aortic allograft transplantation in rats |
Authors: | PEPINELI, Rafael; SANTANA, Alexandre C.; SILVA, Filipe M. O.; TAVONI, Thauany M.; STOLF, Noedir A. G.; NORONHA, Irene L.; MARANHAO, Raul C. |
Citation: | JOURNAL OF PHARMACY AND PHARMACOLOGY, v.73, n.8, p.1092-1100, 2021 |
Abstract: | Objectives The aim of this study was to test whether lipid core nanoparticles loaded with paclitaxel (LDE-PTX) protect rat aortic allograft from immunological damage. Methods Fisher and Lewis rats were used differing in minor histocompatibility loci. Sixteen Lewis rats were allocated to four-animal groups: SYNG (syngeneic), Lewis rats receiving aorta grafts from Lewis rats; ALLO (allogeneic), Lewis rats receiving aortas from Fisher rats; ALLO+LDE (allogeneic transplant treated with LDE), Lewis rats receiving aortas from Fisher rats, treated with LDE (weekly injection for 3 weeks); ALLO+LDE-PTX (allogeneic transplant treated with LDE-PTX), Lewis rats receiving aortas from Fisher rats treated with LDE-PTX (4 mg/kg weekly for 3 weeks). Treatments began on transplantation day. Results Thirty days post-transplantation, SYNG showed intact aortas. ALLO and ALLO+LDE presented intense neointimal formation. In ALLO+LDE-PTX, treatment inhibited neointimal formation; narrowing of aortic lumen was prevented in ALLO and ALLO+LDE. LDE-PTX strongly inhibited proliferation and intimal invasion by smooth muscle cells, diminished 4-fold presence of apoptotic/dead cells in the intima, reduced the invasion of aorta by macrophages and T-cells and gene expression of pro-inflammatory tumour necrosis factor-alpha (TNF alpha), interferon gamma (IFN gamma) and interleukin-1 beta (IL-1 beta). Conclusions LDE-PTX was effective in preventing the vasculopathy associated with rejection and may offer a potent therapeutic tool for post-transplantation. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/11 Artigos e Materiais de Revistas Científicas - LIM/29 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_PEPINELI_Use_of_paclitaxel_carried_in_lipid_nanoparticles_to_2021.PDF Restricted Access | publishedVersion (English) | 3.43 MB | Adobe PDF | View/Open Request a copy |
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