LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental
URI Permanente desta comunidade
O Laboratório de Pesquisa em Cirurgia Experimental é ligado ao Departamento de Cirurgia da Faculdade de Medicina da Universidade de São Paulo (FMUSP).
Linhas de pesquisa: trauma, choque, sepse, substitutos artificiais do sangue, isquemia e reperfusão, disfunção de múltiplos órgãos, transplantes, pré-condicionamento isquêmico; células-tronco na paraplegia; substitutos vasculares; reposição volêmica; soluções hiperténicas; perfusão esplâncnica; marcadores prognósticos de tumor; angiogênese; imunopatologia de modelos experimentais; imunologias de modelos experimentais; cirurgia minimamente invasiva; oncologia experimental; morfologia e biomecânica da aorta.
Site oficial: http://limhc.fm.usp.br/portal/lim26-laboratorio-de-pesquisa-em-cirurgia-experimental/
Linhas de pesquisa: trauma, choque, sepse, substitutos artificiais do sangue, isquemia e reperfusão, disfunção de múltiplos órgãos, transplantes, pré-condicionamento isquêmico; células-tronco na paraplegia; substitutos vasculares; reposição volêmica; soluções hiperténicas; perfusão esplâncnica; marcadores prognósticos de tumor; angiogênese; imunopatologia de modelos experimentais; imunologias de modelos experimentais; cirurgia minimamente invasiva; oncologia experimental; morfologia e biomecânica da aorta.
Site oficial: http://limhc.fm.usp.br/portal/lim26-laboratorio-de-pesquisa-em-cirurgia-experimental/
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- LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental
- LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental
- LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental
Submissões Recentes
A captação de recursos através de agências governamentais
(2023) FACANALI JUNIOR, Marcio Roberto; ARTIFON, Everson Luiz Almeida
Judicialização nos processos das emendas parlamentares e outros recursos da saúde
(2023) MATHEUS, Sidney; ARTIFON, Everson Luiz Almeida
Vitamin C Inhibits Lipopolysaccharide-Induced Hyperinflammatory State of Chronic Myeloid Leukemia Cells through Purinergic Signaling and Autophagy
(2024) PIRES, Daniela A.; BRANDAO-RANGEL, Maysa A. R.; SILVA-REIS, Anamei; OLIMPIO, Fabiana R. S.; AIMBIRE, Flavio; OLIVEIRA, Carlos R.; MATEUS-SILVA, Jose R.; ZAMARIOLI, Lucas S.; BACHI, Andre L. L.; BELLA, Yanesko F.; SANTOS, Juliana M. B.; BINCOLETTO, Claudia; JR, Antonio Herbert Lancha; VIEIRA, Rodolfo P.
Background: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the overproduction of white blood cells, leading to symptoms such as fatigue, infections, and other complications. CML patients must take measures to prevent infections to mitigate the exacerbation of cancer cell proliferation and comorbidities. Methods: This study investigated whether vitamin C can suppress the hyperinflammatory activation of K-562 cells induced by lipopolysaccharide (LPS) and whether purinergic signaling (ATP and P2X7 receptor) and autophagy play a role in it. Two different doses of vitamin C (5 mu g/mL and 10 mu g/mL) were employed, along with the lysosome inhibitor chloroquine (CQ; 100 mu M), administered 2 h prior to LPS stimulation (10 ng/mL) for a duration of 22 h in K-562 cells (3 x 10(5) cells/mL/well). Results: Both doses of vitamin C reduced the release of interleukin-6 (IL-6) (5 mu g/mL, p < 0.01 and 10 g/mL, p < 0.01) and tumor necrosis factor (TNF) (5 g/mL, p < 0.01 and 10 g/mL, p < 0.01) induced by LPS. Furthermore, in LPS + CQ-stimulated cells, vitamin C at a concentration of 10 g/mL inhibited the expression of LC3-II (p < 0.05). Conversely, both doses of vitamin C led to the release of the anti-inflammatory cytokine interleukin-10 (IL-10) (5 g/mL, p < 0.01 and 10 g/mL, p < 0.01), while only the 10 g/mL dose of vitamin C induced the release of Klotho (10 mu g/mL, p < 0.01). In addition, both doses of vitamin C reduced the accumulation of ATP (5 g/mL, p < 0.01 and 10 g/mL, p < 0.01) and decreased the expression of the P2X7 receptor at the mRNA level. Conclusions: Vitamin C inhibits the hyperinflammatory state induced by LPS in K-562 cells, primarily by inhibiting the ATP accumulation, P2X7 receptor expression, and autophagy signaling.
Data-driven, cross-disciplinary collaboration: lessons learned at the largest academic health center in Latin America during the COVID-19 pandemic
(2024) RITTO, Ana Paula; ARAUJO, Adriana Ladeira de; CARVALHO, Carlos Roberto Ribeiro de; SOUZA, Heraldo Possolo De; FAVARETTO, Patricia Manga e Silva; SABOYA, Vivian Renata Boldrim; GARCIA, Michelle Louvaes; KULIKOWSKI, Leslie Domenici; KALLAS, Esper Georges; PEREIRA, Antonio Jose Rodrigues; COBELLO JUNIOR, Vilson; SILVA, Katia Regina; ABDALLA, Eidi Raquel Franco; SEGURADO, Aluisio Augusto Cotrim; SABINO, Ester Cerdeira; RIBEIRO JUNIOR, Ulysses; FRANCISCO, Rossana Pulcineli Vieira; MIETHKE-MORAIS, Anna; LEVIN, Anna Sara Shafferman; SAWAMURA, Marcio Valente Yamada; FERREIRA, Juliana Carvalho; SILVA, Clovis Artur; MAUAD, Thais; GOUVEIA, Nelson da Cruz; LETAIF, Leila Suemi Harima; BEGO, Marco Antonio; BATTISTELLA, Linamara Rizzo; DUARTE, Alberto Jose da Silva; SEELAENDER, Marilia Cerqueira Leite; MARCHINI, Julio; FORLENZA, Orestes Vicente; ROCHA, Vanderson Geraldo; MENDES-CORREA, Maria Cassia; COSTA, Silvia Figueiredo; CERRI, Giovanni Guido; BONFA, Eloisa Silva Dutra de Oliveira; CHAMMAS, Roger; BARROS FILHO, Tarcisio Eloy Pessoa de; BUSATTO FILHO, Geraldo
Introduction The COVID-19 pandemic has prompted global research efforts to reduce infection impact, highlighting the potential of cross-disciplinary collaboration to enhance research quality and efficiency.Methods At the FMUSP-HC academic health system, we implemented innovative flow management routines for collecting, organizing and analyzing demographic data, COVID-related data and biological materials from over 4,500 patients with confirmed SARS-CoV-2 infection hospitalized from 2020 to 2022. This strategy was mainly planned in three areas: organizing a database with data from the hospitalizations; setting-up a multidisciplinary taskforce to conduct follow-up assessments after discharge; and organizing a biobank. Additionally, a COVID-19 curated collection was created within the institutional digital library of academic papers to map the research output.Results Over the course of the experience, the possible benefits and challenges of this type of research support approach were identified and discussed, leading to a set of recommended strategies to enhance collaboration within the research institution. Demographic and clinical data from COVID-19 hospitalizations were compiled in a database including adults and a minority of children and adolescents with laboratory confirmed COVID-19, covering 2020-2022, with approximately 350 fields per patient. To date, this database has been used in 16 published studies. Additionally, we assessed 700 adults 6 to 11 months after hospitalization through comprehensive, multidisciplinary in-person evaluations; this database, comprising around 2000 fields per subject, was used in 15 publications. Furthermore, thousands of blood samples collected during the acute phase and follow-up assessments remain stored for future investigations. To date, more than 3,700 aliquots have been used in ongoing research investigating various aspects of COVID-19. Lastly, the mapping of the overall research output revealed that between 2020 and 2022 our academic system produced 1,394 scientific articles on COVID-19.Discussion Research is a crucial component of an effective epidemic response, and the preparation process should include a well-defined plan for organizing and sharing resources. The initiatives described in the present paper were successful in our aim to foster large-scale research in our institution. Although a single model may not be appropriate for all contexts, cross-disciplinary collaboration and open data sharing should make health research systems more efficient to generate the best evidence.
Effect of Menstrual Cycle Phase on Fuel Oxidation Post HIT in Women Reproductive Age: A Pilot Study
(2023) FRIENTES, C. S.; MARQUEZI, M. L.; APARECIDO, J. M. L.; CASCAPERA, M. S.; ROGERI, P. S.; LANCHA JUNIOR, A. H.
Women of childbearing age have variations in substrate oxidation rates that can lead to overweight, type II diabetes, and other conditions that may be associated with metabolic inflexibility and the variations in estrogen concentrations observed during the monthly ovarian cycle. Purpose: This study aimed to verify and compare the influence of eight treadmill high-intensity interval training (HIT) sessions on carbohydrate and lipid oxidation rates (CHOox and LIPox, respectively) and intensities of ventilatory anaerobic thresholds (VATs) of women in different phases of the monthly ovarian cycle. Methods: Eleven irregularly active women performed incremental treadmill exercise testing followed by submaximal work-rate running for 45 min to determine VATs, VO2peak, peak velocity (Vpeak), and substrate oxidation rates, before and after a training period, in different phases of their monthly ovarian cycle (follicular phase group, FL, n = 6; luteal phase group, LT, n = 5). The training period consisted of eight HIT sessions, composed each one of eight sets of 60 s running at 100%Vpeak interspersed by 75 s recovery every 48 h. Results: Our results showed no significant differences in VATs intensities between groups. The comparison between groups showed significant differences in relative energy derived from CHO pre- and post-training of −61.42% and −59.26%, respectively, and LIP pre- and post-training of 27.46% and 34.41%, respectively. The relative energy derived from CHO after the training period was 18.89% and 25.50% higher for FL and LT, respectively; consequently, the relative energy derived from LIPox after the training period was 8,45% and 3.46% lower for FL and LT, respectively. Over the training period, Vpeak was ~13.5 km/h, which produced the relative intensities of ~89%VO2peak e ~93%HRpeak for both groups. Conclusion: The monthly ovarian cycle phases promote significant changes in substrate oxidation rates leading to a decrease in CHOox. High-intensity interval training can minimize the differences observed and constitute an alternative intervention.
Anthracosis particulate matter causes changes in macrophages inflammatory response and in expression of genes related to xenobiotics metabolism in co-culture of macrophages with BEAS-2B airway cells
(2023) FRIAS, Daniela Perroni; VIEIRA, Gabriela Lima; SMELAN, Juliana; LACERDA, Joao Paulo Amorim De; MAUAD, Thais; DOLHNIKOFF, Marisa; MACCHIONE, Mariangela
Mapping of eosinophils, immune cell profiles, and microbes in COPD lung tissue
(2023) LINDO, Caroline; SIDDHURAJ, Premkumar; MAUCH, Renan; JONSSON, Jimmie; AHLGREN, Johan; JOGDAND, Prajakta; ANDERSSON, Cecilia; CANN, Jennifer; NAZAROFF, Christopher; LINDSTEDT, Sandra; MAUAD, Thais; SANDEN, Caroline; CRAE, Christopher Mc; ERJEFALT, Jonas S.
Expression patterns of Interleukin-33 and its receptor ST2 in severe COVID-19
(2023) LINDO, Caroline; COSTA, Natalia De Souza Xavier; SIDDHURAJ, Premkumar; JONSSON, Jimmie; ORENGO, Jamie M.; SLEEMAN, Matthew A.; LINDSTEDT, Sandra; ANDERSSON, Cecilia; SILVA, Luiz Fernando; DUARTENETO, Armaro Nunes; SALDIVA, Paulo Hilario Nascimento; SANDEN, Caroline; MAUAD, Thais; ERJEFALT, Jonas
Lung ECM composition, its influence factors and transcriptomics in the lungs of severe COVID-19.
(2023) COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; NASCIMENTO, Ellen Toledo Do; BRITO, Jose Mara De; MONTEIRO, Jhonatas Sirino; SETUBAL, Joao Carlos; PINHO, Joao Renato Rebello; PEREIRA, Roberta Verciano; MONTEIRO, Renata Aparecida De Almeida; DUARTE NETO, Amaro Nunes; SALDIVA, Paulo Hilario Nascimento; SILVA, Luiz Fernando Ferraz Da; DOLHNIKOFF, Marisa; MAUAD, Thais
Green Areas and PM2,5 exposure could be associated with Asthma and COPD?
(2023) ALMEIDA, Francine Maria; MOREIRA, Tiana Carla Lopes; OLIVEIRA, Lucas Miranda; RIGHETTI, Renato Fraga; LOPES, Fernanda Degobbi Tenorio Quirino Santos; ALENCAR, Airlane P.; GOUVEIA, Nelson; MAUAD, Thais; LOTUFO, Paulo A.; BENSENOR, Isabela; SANTOS, Itamar Souza; TIBERIO, Iolanda Fatima Lopes Calvo