MARIANGELA MACCHIONE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles(2021) SERIANI, Robson; PAULA, Carla Peres de; CUNHA, Anderson Ferreira da; OLIVEIRA, Marcos Antonio de; KREMPEL, Paloma Gava; FRIAS, Daniela Perroni; NEGRI, Elnara Marcia; MAUAD, Thais; MACCHIONE, MariangelaSeveral mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 mu g/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 +/- 0.34; *p = 0.0220), PRDX5 (0.43 +/- 0.34; *p = 0.0220), and PRDX6 (0.33 +/- 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.
- Low-dose chlorine exposure impairs lung function, inflammation and oxidative stress in mice(2021) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; KUNZLER, Deborah De Camargo Hizume; TRIPODE, Bruna Gabryela Busoletto; KURDEJAK, Adriana; CORDEIRO, Bruna Nakamura; PANDOLPHO, Renata; MACCHIONE, Mariangela; BRUGGEMANN, Thayse Regina; VIEIRA, Rodolfo Paula; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; SARAIVA-ROMANHOLO, Beatriz MangueiraAim: To explore the different consequences of acute and chronic exposure to chlorine gas (Cl-2) on the functional and histological parameters of health mice. Main methods: Firstly, male BALB/c mice were acute exposed to 3.3 or 33.3 or 70.5 mg/m(3) Cl-2. We analyzed the lung function, the inflammatory cells in the bronchoalveolar lavage, cell influx in the peribrochoalveolar space and mucus production. In a second phase, mice were chronic exposed to 70.5 mg/m(3) Cl-2. Besides the first phase analyses, we also evaluated the epithelial cells thickness, collagen deposition in the airways, immunohisto-chemistry stain for IL-1 beta, iNOS, IL-17 and ROCK-2 and the levels of IL-5, IL-13, IL-17, IL-1 beta and TNF-alpha in lung homogenate. Key findings: Acute exposure to chlorine impaired the lung function, increased the number of inflammatory cells in the BALF and in the airways, also increased the mucus production. Furthermore, when chlorine was exposed chronically, increased the airway remodeling with collagen deposition and epithelial cells thickness, positive cells for IL-1 beta, iNOS, IL-17 in the airways and in the alveolar walls and ROCK-2 in the alveolar walls, lung inflammation with increased levels of IL-5, IL-13, IL-1 beta and TNF-alpha in the lung homogenate, and also, induced the acid mucus production by the nasal epithelium. Significance: Acute and chronic exposure to low dose of chlorine gas worsens lung function, induces oxidative stress activation and mucus production and contributes to augmenting inflammation in health mice.
- Effectiveness of the 40-Minute Handmade Manikin Program to Teach Hands-on Cardiopulmonary Resuscitation at School Communities(2021) NAKAGAWA, Naomi K.; OLIVEIRA, Katia M. G.; LOCKEY, Andrew; SEMERARO, Federico; AIKAWA, Priscila; MACCHIONE, Mariangela; CARVALHO-OLIVEIRA, Regiani; GOUVEA, Gabriela B.; BOAVENTURA, Ana Paula; I, Adalgisa Maiworm; CALDERARO, Marcelo; HAJJAR, Ludhmila A.; V, Eduardo Motta; SOUZA, Heraldo P.; ANDRE, Carmen D. S. de; SILVA, Luiz F. F.; POLASTRI, Thatiane F.; TIMERMAN, Sergio; CARMONA, Maria Jose C.; BOETTIGER, Bernd W.Bystander training in cardiopulmonary resuscitation (CPR) is crucial to improve the victims' survival and quality of life after sudden cardiac arrest. This observational study aimed to determine the success rate of 2 different programs of CPR training for children, adolescents, and adults in school communities. We assessed the development and acquisition of the following CPR skills checking local safety, assessing victim's responsiveness, calling for help, assessing victim's breathing, and performing chest compression (hands and straight arms placement on the chest, compression velocity, depth, and chest release) using a 40-minute program with handmade manikins or the 120-minute program using intermediate-fidelity manikins. There were 1,630 learners (mean age 16 years, 38% male) in the 40-minute program, and 347 learners (mean age 27 years, 32% male) in the 120-minute program. The lowest successful pass rate of learners that developed CPR skills was 89.4% in the 40-minute program and 84.5% in the 120-minute program. The chances of success increased with age in the same program (compression rate and depth). The success rate also increased with the more extended and intermediate-cost program at the same age (assessing victim's responsiveness, calling for help, and assessing the victim's respiration). In conclusion, a 40-minute and cheaper (low-cost handmade manikin) CPR program was adequate to develop and acquire the overall CPR skills for >= 89% at school communities, independently of gender. However, some individual CPR skills can be further improved with increasing age and using the longer and intermediate-cost program.
- Effects of intrauterine exposure to concentrated ambient particles on allergic sensitization in juvenile mice(2021) BRITO, Jose Mara de; AMEIDA, Francine Maria de; ARANTES-COSTA, Fernanda Magalhaes; GUIMARAES, Eliane Tigre; MORGAN, Adriana; MANGONE, Flavia Rotea; PAVANELLI, Ana Carolina; NAGAI, Maria Aparecida; VIEIRA, Rodolfo P.; MACCHIONE, Mariangela; MAUAD, ThaisIntrauterine exposure to particulate matter (PM) has been associated with an increased risk of asthma development, which may differ by the age of asthma onset, sex, and pollutant concentration. To investigate the pulmonary effects of in utero exposure to concentrated urban ambient particles (CAPs) in response to house dust mite (HDM) sensitization in juvenile mice. Mice were exposed to CAPs (600 mu g/m(3) PM2.5) during the gestational period. Twenty-two-day postnatal mice were sensitized with HDM (100 mu g, intranasally, 3 times per week). Airway responsiveness (AHR), serum immunoglobulin, and lung inflammation were assessed after 43 days of the postnatal period. Female (n = 47) and male (n = 43) mice were divided into four groups as follows: (1) FA: not exposed to CAPs; (2) CAPs: exposed to CAPs; (3) HDM: sensitized to HDM; and (4) CAPs+HDM: exposed to CAPs and HDM-sensitized. PM2.5 exposure did not worsen lung hyperresponsiveness or allergic inflammation in sensitized animals. The levels of the lung cytokines IL-4, TNF-alpha, and IL-2 were differentially altered in male and female animals. Males presented hyporesponsiveness and increased lung macrophagic inflammation. There were no epigenetic changes in the IL-4 gene. In conclusion, intrauterine exposure ambient PM2.5 did not worsened allergic pulmonary susceptibility but affected the pulmonary immune profile and lung function, which differed by sex.