RICARDO APARECIDO BAPTISTA NUCCI
Projetos de Pesquisa
Unidades Organizacionais
LIM/66, Hospital das Clínicas, Faculdade de Medicina
16 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 16
- Effect of Resistance Training and Diet Intake on Spleen Structure of Ovariectomized Wistar Rats(2022) SANTANA, Erika Steffany dos Santos; OLIVEIRA, Carolinne Alves de; LIMA, Francisca Iranni Alves; NUCCI, Ricardo Aparecido Baptista; FONSECA, Fernando Luiz Affonso; MAIFRINO, Laura Beatriz MesianoIntroduction Exercise is a key factor in immunity which may be affected by a different diet intake. Thus, we aimed to analyze the effects of diet intake and resistance training on spleen structure of ovariectomized female animals. Materials and Methods Female Wistar rats were divided into eight experimental groups: sedentary and nonovariectomized animals plus vegetable protein diet (CVS) or animal protein diet (CAS); trained and nonovariectomized rats plus vegetable protein diet (CVT) or animal protein diet (CT); sedentary and ovariectomized groups plus vegetable protein diet (VOS) or animal protein diet (AOS); and trained and ovariectomized animals plus vegetable protein diet (VOT) or animal protein diet (AOT). Results Groups submitted to both animal protein diet and resistance training, mainly ovariectomized groups, presented a great variability of collagen fibers type III, white pulp, and follicle structure in relation to the other spleen constituents. Conclusion Resistance training with a vegetable protein diet may play a key factor to maintain spleen's immune responses across age. However, animal protein diet is suggested to decrease spleen's immune activity.
- Evaluation of the diaphragm muscle remodeling, inflammation, oxidative stress and vascularization in smokers: An autopsy study(2020) NUCCI, R.A.B.; MAIFRINO, L.B.M.; BUSSE, A.L.; SOUZA, R.R. De; PASQUALUCCI, C.A.; ANARUMA, C.A.; LEITE, R.E.P.; RODRIGUEZ, R.D.; SUEMOTO, C.K.; JACOB-FILHO, W.Background/Aims: Cigarette smoking is a key factor in systemic inflammation and oxidative stress, and it has also been associated with the loss of muscle strength and an elevated risk of pulmonary diseases. Thus, this study aimed to analyze the effects of cigarette smoking on the diaphragm muscle structure of postmortem samples. Methods: Immunohistochemical techniques were used for muscle remodeling (metalloproteinases 2 and 9), inflammation (cyclooxygenase-2), oxidative stress (8-hydroxy-2'-deoxyguanosine), and vascularization (vascular endothelial growth factor). Hematoxylin and eosin stain was used for histopathological analysis and Picrosirius stain was used to highlight the collagen fibers. Results: Cigarette smokers had an increase of diaphragm muscle remodeling, oxidative stress, inflammation, and vascularization compared to non-smokers. Conclusion: Diaphragm muscle structure may be negatively affected by cigarette smoking. © 2020 The Author(s).
- Remodeling of the soleus muscle of ovariectomized old female rats submitted to resistance training and different diet intake(2020) BRAGGION, Glaucia Figueiredo; ORNELAS, Elisabete de Marco; CURY, Jurema Carmona Sattin; SOUSA, Jessica Pedroso de; NUCCI, Ricardo Aparecido Baptista; FONSECA, Fernando Luiz Affonso; MAIFRINO, Laura Beatriz MesianoSarcopenia is a common condition that is associated mainly with hormonal factors, nutritional status, physical activity, leading to a lower quality of life. Thus, this study aimed to evaluate the effects of diets with vegetable or animal proteins (AP) associated with resistance training on the structure of the soleus muscle in aged Wistar rats. The histochemical technique was used for the typing of muscle fibers, the cross-sectional area of myocytes, and volume densities of myocytes and interstitium. Picrosirius stain was used to quantify the collagen density. Diet intake, mainly animal protein, associated with resistance training leaded to muscle remodeling, and increased deposit of collagen fibers. We observed hypertrophy in animal groups that consumed animal protein diet, even the sedentary group, although more evident in those trained.
- Ultrastructural effects of diabetes in the right atrium cardiomyocytes of elderly Wistar rats(2020) ANDRADE, Natalie Souza de; CHUCATA, Kemily Loren Barros; MAGALHAES, Walkyria Villegas; NUCCI, Ricardo Aparecido Baptista; COSTA-SANTOS, Nicolas Da; DIAS, Igor Roberto; LIMA, Hunter Douglas de Souza; MAIFRINO, Laura Beatriz Mesiano; SOUZA, Romeu Rodrigues deThe present study aimed to evaluate the effects of diabetes on quantitative parameters of right atrial cardiomyocytes of elderly rats. Wistar rats (14 months of age) were divided into two groups: streptozotocin-diabetic rats (DG) and control rats (CG). The groups were sacrificed at 16 months. Ultrafine sections of the right atrium were analyzed by electron microscopy. In elderly diabetic animals, histograms of the frequency distribution of natriuretic peptides according to their size showed increased number of small and medium peptides in relation to large peptides, which increased its numerical density leading to a decrease in the mean diameter of both natriuretic peptides. However, elderly diabetic animals remained normotensive. No significant difference was observed between the groups for the volume density of mitochondria, endoplasmic reticulum, and Golgi apparatus. In conclusion, elderly diabetic rats showed increased functional activity of atrial cardiomyocytes with greater production of natriuretic peptides in association with a quantitative maintenance of cytoplasmic components.
- Age-Related Effects on Right Femoral Bone of Male Wistar Rats: A Morphometric and Biomechanical Study(2022) PUELKER, Sheila Martins; CASTRO, Sonia Regina Ribeiro de; SOUZA, Romeu Rodrigues de; MAIFRINO, Laura Beatriz Mesiano; NUCCI, Ricardo Aparecido Baptista; SITTA, Maria do CarmoIntroduction Study of the variations of bone characteristics with age in different animal models is important to design musculoskeletal studies. Thus, this study aimed to evaluate the bone mass, dimensions, and biomechanical parameters of the femur in young, middle-aged, and aged Wistar rats. Materials and Methods Thirty male rats ( Rattus norvegicus ) were divided in three groups ( n = 10 per group)-3-month-old young rats, 12-month-old middle-aged rats, and 18-months-old aged rats. The right femurs were subjected sequentially to morphometric study (bone weight, cortical thickness) and biomechanical tests (maximum resistance strength and bone stiffness). Results We observed a significant increase in femur histological (cortical thickness) and biomechanical (maximum strength and bone stiffness) parameters with aging when compared with young animals. Conclusions With the advancing age, the right femoral bone of middle-aged and old animals had greater variations when compared with young animals. However, further studies with the aid of a comparison between right and left femur and other long bones in both male and female rats are needed to corroborate with our findings.
- Effects of resistance training on kidney morphology of aged ovariectomized rats(2020) MARCELINO, Marcelo Cristiano da Silva; MAGALHAES, Walkyria Villegas; FONSECA, Fernando Luiz Afonso; NUCCI, Ricardo Aparecido Baptista; MAIFRINO, Laura Beatriz MesianoWith the advancing age, there is an increase of the incidence of chronic renal diseases. However, it is suggested that exercise, as resistance training, is a key factor in positively modulate the kidney structure and function. Thus, this study aimed to analyze the effects of estrogen deprivation and a moderate resistance protocol on the right kidney structure of rats. A total of 32 animals were randomly divided in four groups (n = 8/per group): control group (CG); resistance trained animals (RT); control ovariectomized animals (OvxC); and trained ovariectomized animals (OvxT). Sections of the right kidney were stained with hematoxylin and eosin for morphometry and blood was collected for biochemical analysis. Our results showed that the group submitted to both ovariectomy and resistance training had significant impact on renal corpuscle components (Bowman's space, renal corpuscle area, and visceral layer), and proximal and distal tubules. Additionally, resistance training, with or without estrogen deprivation, increases creatine phosphokinase and decreases ultrasensitive C-reactive protein. In summary, we suggested that moderate resistance training may be a key factor to maintain the kidney structure and function across age. However, future studies are need to corroborate with our findings.
- The ""Long COVID"" Respiratory Symptoms-Concerns with Frailty and Respiratory Diseases(2022) NUCCI, Ricardo Aparecido Baptista; JACOB-FILHO, Wilson
- Age-related changes in the articular cartilage of the mandible of rats(2020) CALDERON, M. F.; NUCCI, R. A. B.; SOUZA, R. R. deThis study investigated the effects of aging on the articular cartilage of the mandible. Wistar rats were divided in two groups (n = 10/per group): 3-months-old group (young group); and 13-months-old group (aged group). After euthanasia, the head of the mandible was collected and stained with hematoxylin and eosin (HE) to evaluate the thickness of the articular layer and cartilage. Sections stained with Picrosirius red and Safranin O were used to evaluate the collagen and proteoglycans deposition, respectively. First, aging has decreased the articular layer thickness. Second, the results suggest a decrease of chondrocytes followed by an increase of the matrix to maintain the mandible homeostasis. Finally, both collagen and proteoglycans increased with aging. Aging displayed important effects to the mandible of aged rats.
- Effects of different diet intake and resistance training on left ventricle remodeling in ovariectomized rats(2019) CURY, J.C.S.; ENCINAS, J. de Araújo; NUCCI, R.A.B.; ORNELAS, E. de Marco; LIMA, N.E.A. de; BRAGGION, G.F.; ALVES, B. da Costa Aguiar; VEIGA, G.L. da; FONSECA, F.L.A.; MAIFRINO, L.B.M.It is known that physical activity promotes positive physiological adaptations, especially in cardiovascular responses in individuals of different age groups; however, it is necessary to know if different diet influences the effects of exercise on the left ventricular remodeling. Its aim is to evaluate the effects of resistance training associated with vegetable and animal protein diets on the left ventricular remodeling of ovariectomized aged animals. Female Wistar rats were divided into eight experimental groups: sedentary and non-ovariectomized animals plus vegetable protein diet (CVS) or animal protein diet (CAS); trained and non-ovariectomized rats plus vegetable protein diet (CVT) or animal protein diet (CAT); sedentary and ovariectomized groups plus vegetable protein diet (VOS) or animal protein diet (AOS); trained and ovariectomized animals plus vegetable protein diet (VOT) or animal protein diet (AOT). Ovariectomy promoted an increase in the density of collagen type I and III in all animals, being more marked in the groups of vegetal protein diet (p < 0.05). Additionally, our ultrastructural analysis demonstrated a greater amount of type I collagen fibers and a decrease of type III collagen fibers in the sedentary ovariectomized animals regardless of the diet consumed. We can observe that VOT and AOT had a significant decrease (p < 0.05) of MMP-2 when compared to the other groups. In addition, both training and/or ovariectomized groups demonstrated an increase in MMP-9. We observed the benefits of resistance training associated mainly with the animal diet on cardiac tissue, highlighting the importance of these interventions in the prevention and reversal of changes in the various organs and systems resulting from menopause. © 2019, Springer-Verlag London Ltd., part of Springer Nature.
- Diaphragm muscle structure in the elderly: Findings from an autopsy study(2020) NUCCI, Ricardo Aparecido Baptista; SOUZA, Romeu Rodrigues de; SUEMOTO, Claudia Kimie; BUSSE, Alexandre Leopold; MAIFRINO, Laura Beatriz Mesiano; ANARUMA, Carlos Alberto; PASQUALUCCI, Carlos Augusto; JACOB-FILHO, WilsonSarcopenia is characterized as aging-related weakness and atrophy, which decreases the diaphragm force generating capacity leading to a decrease of expulsive non-ventilatory motor behaviors, which are critical for airway clearance. Thus, this study aimed to analyze the histopathology of the diaphragm in postmortem samples. Thirty individuals were included. Diaphragm samples were stained with hematoxylin and eosin for histopathological analysis. Picrosirius stain was used to highlight the collagen fibers. We observed a positive association between advancing age and histopathological findings in the diaphragm structure. We suggest that age is a key factor in increasing diaphragm muscle histopathology. However, further clinicopathological studies are needed to confirm our findings.