SONIA DE FATIMA SOTO YOSHIDA
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
20 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 20
- Tacrolimus impairs airway mucociliary clearance of rats(2024) SILVA, Maristela Prado E.; SOTO, Sonia de Fatima; ALMEIDA, Francine Maria de; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo Manuel; PAZETTI, RogerioObjectives: Tacrolimus (TAC) is the most widely used immunosuppressive agent after lung transplantation. Considering that the ciliary beat frequency (CBF) mainly depends on the cytoplasmic calcium concentration and that TAC can affect this due to its binding with the intracellular immunophilin FKBP12, we hypothesized that TAC could also impair the airway mucociliary clearance of rats. Methods: Sixty rats were divided into two groups (n = 30 each): Control = water; TAC = tacrolimus. After 7, 15 or 30 days of treatment, ten animals from each group were euthanized and the following parameters were studied: mucus transportability, CBF, mucociliary transport velocity (MCTV), and neutral and acid mucus production. Results: There was a significant decrease in CBF (Control vs TAC: 7 days, p = 0.008; 15 days, p = 0.007; 30 days, p = 0.001) and MCTV (Control vs TAC: 7 days, p = 0.004; 15 days, p < 0.001; 30 days, p < 0.001) in all immunosuppressed animals. TAC therapy also caused an increase in acid mucus production at all treatment times (Control vs TAC: 7 days, p = 0.001; 15 days, p = 0.043; 30 days, p = 0.001). Conclusions: TAC impairs airway mucociliary clearance of rats.
conferenceObject Side effects of tacrolimus upon airway epithelial tissue(2013) SILVA, Maristela Prado e; SOTO, Sonia; ALMEIDA, Francine; LIMONETE, Tatiana; PARRA, Edwin; PEGO-FERNANDES, Paulo; JATENE, Fabio; PAZETTI, RogerioconferenceObject Fine particulate matter in the inspired air within the limits recommended by WHO alters placental structure(2014) SOTO, Sonia; LOPES, Karen; VERAS, Mariana; HEIMANN, JoelconferenceObject Comparison of two immunosuppressant triple therapies on airway mucociliary clearance in rats(2012) SILVA, Maristela Prado e; SOTO, Sonia; ALMEIDA, Francine; LIMONETE, Tatiana; PARRA, Edwin; PEGO-FERNANDES, Paulo; JATENE, Fabio; PAZETTI, RogerioconferenceObject Fine particulate matter in the air within the limits recommended by WHO alters placental structure(2015) SOTO, Sonia; MARCHESI, Guilherme; LOPES, Karen; VERAS, Mariana; HEIMANN, Joel- Immunosuppression Effects on Airway Mucociliary Clearance: Comparison Between Two Triple Therapies(2013) SILVA, Maristela Prado e; SOTO, Sonia F.; ALMEIDA, Francine M.; LIMONETE, Tatiana T. K.; PARRA, Edwin R. C.; JATENE, Fabio B.; PEGO-FERNANDES, Paulo M.; PAZETTI, RogerioBackground. Tacrolimus and mycophenolate have now become the most widely used combination for maintenance immunosuppressive regimens after lung transplantation in comparison with cyclosporine and azathioprine. However, limited information is available with respect to their effects on cells, other than those from the immunologic compartment. We hypothesized that different triple therapies could have different effects on airway mucociliary clearance, playing an important role in respiratory infections observed after lung transplantation. Methods. Ninety rats were assigned to three groups (n = 30 each): control = vehicle, therapy 1 = tacrolimus + mycophenolate + prednisone, and therapy 2 = cyclosporine + azathioprine + prednisone. After 7, 15, or 30 days of treatment by gavage, the animals were killed and the following parameters were studied: mucus transportability, ciliary beating frequency, mucociliary transport velocity, and neutral and acid mucus production. Results. There was a significant decrease in ciliary beating frequency, mucociliary transport velocity, and neutral mucus production in all immunosuppressed animals; indeed, both therapies, mainly therapy 1, caused an increase in acid mucus production for as long as 15 days of treatment. Conclusions. Both triple therapies impaired airway mucociliary clearance of rats, but therapy 1 had a more deleterious effect. These data suggest that these undesirable effects can contribute to the high incidence of respiratory infections observed in patients undergoing lung transplantation. (C) 2013 by The Society of Thoracic Surgeons
conferenceObject Effects of exposure do air pollution on placental structure(2013) SOTO, Sonia de Fatima; LOPES, Karen; VERAS, Mariana Matera; HEIMANN, JoelconferenceObject Tacrolimus increases inflammatory cells in lung(2014) ALMEIDA, Francine Maria; SILVA, Maristela Prado e; SOTO, Sonia Fatima; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo; PAZETTI, Rogerio- Exposure to fine particulate matter in the air alters placental structure and the renin-angiotensin system(2017) SOTO, Sonia de Fatima; MELO, Juliana Oliveira de; MARCHESI, Guilherme D'Aprile; LOPES, Karen Lucasechi; VERAS, Mariana Matera; OLIVEIRA, Ivone Braga de; SOUZA, Regiane Machado de; CASTRO, Isac de; FURUKAWA, Luzia Naoko Shinohara; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel C.Transforming growth factor beta 1 (TGF beta 1), the uteroplacental renin-angiotensin system (RAS) and vascular endothelial growth factor A (VEGF-A) participate in the placentation process. The aim of this study was to investigate the effects of exposure to pollutants on the placenta. Methods Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning on day 6 of pregnancy. At embryonic day 19, the placenta was collected. The placental structure, the protein and gene expression of TGF beta 1, VEGF-A, and its receptor Flk-1 and RAS were evaluated by indirect ELISA and quantitative real-time PCR. Results Exposure to P decreased the placental mass, size, and surface area as well as the TGF beta 1, VEGF-A and Flk-1 content. In the maternal portion of the placenta, angiotensin II (AngII) and its receptors AT(1) (AT(1)R) and AT(2) (AT(2)R) were decreased in the PF and PP groups. In the fetal portion of the placenta, AngII in the FP, PF and PP groups and AT(2)R in the PF and PP groups were decreased, but AT(1)R was increased in the FP group. VEGF-A gene expression was lower in the PP group than in the FF group. Conclusions Exposure to pollutants before and/or during pregnancy alters some characteristics of the placenta, indicating a possible impairment of trophoblast invasion and placental angiogenesis with possible consequences for the maternal-fetal interaction, such as a limitation of fetal nutrition and growth.
conferenceObject HIGH-SALT INTAKE DURING PREGNANCY MODIFIES PLACENTA PHENOTYPE AT LEAST UNTIL THE THIRD GENERATION(2014) PERES, Maria A.; SOTO, Sonia F.; GUIMARADES, Carina F.; SILVA, Rafaela C. P.; OLIVEIRA, Ivone B. de; FURUKAWA, Luzia N. S.; HEIMANN, Joel C.