PAULO ROBERTO DOS SANTOS SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
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- Hydrolyzed whey protein enriched with glutamine dipeptide attenuates skeletal muscle damage and improves physical exhaustion test performance in triathletes(2023) PERES, Fabiano Pinheiro; LEVADA-PIRES, Adriana Cristina; VIEIRA, Marcelo; HATANAKA, Elaine; CURY-BOAVENTURA, Maria Fernanda; FOLADOR, Alessandra; GORJAO, Renata; HIRABARA, Sandro Massao; SANTOS-SILVA, Paulo Roberto; DEUSTER, Patricia A.; CURI, Rui; PITHON-CURI, Tania CristinaPurposeTo investigate the effects of hydrolyzed whey protein enriched with glutamine dipeptide on the percentage of oxygen consumption, second ventilatory threshold, duration and total distance covered, and skeletal muscle damage during an exhaustion test in elite triathletes. MethodsThe study was a randomized, double-blinded, placebo-controlled, crossover trial. Nine male triathletes performed a progressive incremental test on a treadmill ergometer (1.4 km h(-1)center dot 3 min(-1)) 30 min after ingesting either 50 g of maltodextrin plus four tablets of 700 mg hydrolyzed whey protein enriched with 175 mg of glutamine dipeptide diluted in 250 ml of water (MGln) or four tablets of 700 mg maltodextrin plus 50 g maltodextrin diluted in 250 ml of water (M). Each athlete was submitted to the two dietary treatments and two corresponding exhaustive physical tests with an interval of one week between the interventions. The effects of the two treatments were then compared within the same athlete. Maximal oxygen consumption, percentage of maximal oxygen consumption, second ventilatory threshold, and duration and total distance covered were measured during the exhaustion test. Blood was collected before and immediately after the test for the determination of plasma lactate dehydrogenase (LDH) and creatine kinase (CK) activities and lactate concentration (also measured 6, 10, and 15 min after the test). Plasma cytokines (IL-6, IL-1 beta, TNF-alpha, IL-8, IL-10, and IL-1ra) and C-reactive protein levels were also measured. ResultsA single dose of MGln increased the percentage of maximal oxygen consumption, second ventilatory threshold duration, and total distance covered during the exhaustion test and augmented plasma lactate levels 6 and 15 min after the test. MGln also decreased plasma LDH and CK activities indicating muscle damage protection. Plasma cytokine and C-reactive protein levels did not change across the study periods. ConclusionConditions including overnight fasting and a single dose of MGln supplementation resulted in exercising at a higher percentage of maximal oxygen consumption, a higher second ventilatory threshold, blood lactate levels, and reductions in plasma markers of muscle damage during an exhaustion test in elite triathletes. These findings support oral glutamine supplementation's efficacy in triathletes, but further studies require.
- Moderate levels of physical fitness maintain telomere length in non-senescent T CD8(+) cells of aged men(2020) BASTOS, Marta Ferreira; MATIAS, Manuella de Sousa Toledo; ALONSO, Angelica Castilho; SILVA, Leia Cristina Rodrigues; ARAUJO, Adriana Ladeira de; SILVA, Paulo Roberto; BENARD, Gil; BOCALINI, Danilo Sales; BAKER, Julien Steven; LEME, Luiz Eugenio GarcezOBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4(+) (CD45RO(neg) and CD45RO(+) ), effector CD8(+)CD28(neg), and CD8(+) CD28(+) T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28(+), naive, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4(+) CD45RO(+), CD4(+) CD45RO(neg), CD8(+) CD28(+), and CD8(+) CD28(neg)T cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4(+) cells and longer telomeres in CD8(+) CD28(+) T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28(+) CD8(+)1 cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men.
- Futsal improve body composition and cardiorespiratory fitness in overweight and obese children. A pilot study(2018) SANTOS-SILVA, P. R.; GREVE, J. M. D'Andrea; NOVILLO, H. N. E.; HADDAD, S.; SANTOS, C. R. P.; LEME, R. B.; FRANCO, R. R.; COMINATO, L.; ARAúJO, A. T. M.; SANTOS, F. M.; DAMIANI, D.; RICA, R. L.; BOCALINI, D. S.Aims: The aim of this study was to verify body composition, cardiorespiratory fitness, and biochemical markers of prepubertal overweight and obese boys to a 16-week futsal training program. Methods: Sixteen boys (age: 7-10 y, body mass index>thanat 95th percentileaccording to Center for Disease Control and Prevention; 35.5±7.4 percent fat) participated to futsal training program. The assessment of body composition was estimated using skinfold thickness, and the following variables were evaluated: total body mass, body mass index, body fat percentage, and lean body mass. Aerobic fitness measurementwas performed by gas exchange analysis in treadmill. In addition, an evaluation of the biochemical profile was conducted: triglycerides, total cholesterol, low density lipoprotein cholesterol, high-density lipoprotein cholesterol, plasma concentrations of glucose, and insulin. The futsal intervention included 60-min sessions performed two times/week. The mean intensity during training was between 57 to 88% of maximal heart rate of the age-predicted. Individual portable heart rate monitor controlled training intensity. Results: Significant increases in total body mass (4%), height (3%), lean body mass (8%), and significant 6%-decrease in body fat percentage was observed. Body mass index remained unchanged. Maximal oxygen uptake was elevated (p<0.018) by 11%. Biochemical markers were not modified after intervention. No association was found between body composition and metabolic variables. The effect size of futsal training on most variables was small (<0.5). Conclusion: Controlled intensity and adherence to this 16-week futsal training program were determinant to enhance body composition, and cardiorespiratory fitness in this group of prepubertal boys. © Universidade Estadual Paulista - UNESP. All rights reserved.
conferenceObject RESTING METABOLIC RATE AND WEIGHT LOSS AFTER BARIATRIC SURGERY Integrated Health/Multidisciplinary care(2017) MOTA, F.; CLEVA, R.; GADDUCCI, A.; CARDIA, L.; GREVE, J.; SILVA, P. R.; SANTO, M. A.conferenceObject LACTATE AS MARKER OF METABOLIC SYNDROME IN SEVERE OBESITY Basic science and research in bariatric surgery(2019) VIEIRA, A. Gadducci; CARDIA, L.; OLIVEIRA, F. Costa De; MARIA, J. Greve De; SANTOS, P. Silva Roberto; PAJECKI, D.; AURELIO, M. Santo; CLEVA, R. De- Positive effects of football on fitness, lipid profile, and insulin resistance in Brazilian patients with type 2 diabetes(2014) SOUSA, M. V. de; FUKUI, R.; KRUSTRUP, P.; PEREIRA, R. M. R.; SILVA, P. R. S.; RODRIGUES, A. C.; ANDRADE, J. L. de; HERNANDEZ, A. J.; SILVA, M. E. R. daWe evaluated the effects of recreational football training combined with calorie-restricted diet (football+diet) vs calorie-restricted diet alone (diet) on aerobic fitness, lipid profile, and insulin resistance indicators in type 2 diabetes (T2D) patients. Forty-four T2D patients aged 48-68 years (27 females, 17 males) were randomly allocated to the football+diet group (FDG; n=22) or to the diet group (DG; n=22), of whom 19 FDG and 15 DG subjects completed the study. The football training was performed for 3x40min/week for 12 weeks. Dual-energy X-ray absorptiometry scanning, treadmill testing, and fasting blood samplings were performed pre and post-intervention. After 12 weeks, maximal oxygen uptake (VO2max) was elevated (P<0.05) by 10 +/- 4% in FDG but not in DG (-3 +/- 4%, P<0.05). After 12 weeks, reductions in blood triglycerides (0.4 +/- 0.1mmol/L), total cholesterol (0.6 +/- 0.2mmol/L), low-density lipoprotein, and very low-density lipoprotein levels were observed only in FDG. Fat mass decreased (P<0.05) by 3.4 +/- 0.4kg in FDG and 3.7 +/- 0.4kg in DG. The lower (P<0.05) glucagon and homeostatic model assessment of insulin resistance indicated an improvement in insulin sensitivity in FDG. In conclusion, football combined with restricted diet was effective in enhancing VO2max, reducing total cholesterol and triglycerides, and increasing insulin sensitivity, potentially providing better tools for the prevention of T2D complications than diet alone.
conferenceObject BODY COMPOSITION AND RESTING METABOLIC RATE IN WEIGHT LOSS AFTER BARIATRIC SURGERY(2016) SANTO, M. A.; MOTA, F. C.; V, A. Gadducci; SANTAREM, G. C.; SILVA, P. R.; GREVE, J.; CLEVA, R.- Muscle strength and body composition in severe obesity(2017) GADDUCCI, Alexandre Vieira; CLEVA, Roberto de; SANTAREM, Gabriela Correia de Faria; SILVA, Paulo Roberto Santos; GREVE, Julia Maria D'Andrea; SANTO, Marco AurelioOBJECTIVE: The aim of our study was to evaluate associations between maximum voluntary contraction torques of the lower limbs and body composition for subjects with severe obesity. METHODS: Body composition was evaluated by bioelectrical impedance analysis, and maximum voluntary contraction torques of the lower limbs were measured using an isokinetic dynamometer. One hundred thirty-two patients were enrolled (100 females and 32 males). Eighty-seven patients had a body mass index between 40 and 49.9 kg/m(2) (the A group), and 45 patients had a body mass index between 50 and 59.9 kg/m(2) (the B group). RESULTS: Absolute extension and flexion torques had weak associations with fat-free mass but a moderate association with absolute extension torque and fat-free mass of the lower limbs. There were no significant differences between the A and B groups with respect to absolute extension and flexion torques. For the A group, absolute extension and flexion torques were moderately associated with fat-free mass and with fat-free mass of the lower limbs. For the B group, there were only moderate associations between absolute extension and flexion torques with fat-free mass of the lower limbs. CONCLUSIONS: Our findings demonstrate that both groups exhibited similar absolute torque values. There were weak to moderate associations between absolute extension and flexion torques and fat-free mass but a moderate association with fat-free mass of the lower limbs. Individuals with severe obesity should strive for greater absolute torques, fat-free mass and especially fat-free mass of the lower limbs to prevent functional limitations and physical incapacity.
- Association between muscle strength and the cardiopulmonary status of individuals living with HIV/AIDS(2013) RASO, Vagner; SHEPHARD, Roy J.; CASSEB, Jorge; DUARTE, Alberto Jose da Silva; SILVA, Paulo Roberto Santos; GREVE, Julia Maria D'AndreaOBJECTIVE: The purpose of this study was to compare aerobic function [ anaerobic threshold (%(V) over dotO(2)-AT), respiratory compensation point (%(V) over dotO(2)-RCP) and peak oxygen uptake ((V) over dotO(2peak))] between physically active patients with HIV/AIDS and matched controls and to examine associations between disease status, poor muscle strength, depression (as estimated by the profile of mood states questionnaire) and the aerobic performance of patients. METHODS: Progressive treadmill test data for %(V) over dotO(2)-AT (V-slope method), RCP and ((V) over dotO(2peak)) were compared between 39 male patients with HIV/AIDS (age 40.6 +/- 1.4 years) and 28 male controls (age 44.4 +/- 2.1 years) drawn from the same community and matched for habitual physical activity. Within-patient data were also examined in relation to CD4(+) counts (nadir and current data) and peak isokinetic knee torque. RESULTS: AT, RCP and ((V) over dotO(2peak)) values were generally similar for patients and controls. Within the patient sample, binary classification suggested that AT, RCP and ((V) over dotO(2peak)) values were not associated with either the nadir or current CD4(+) count, but treadmill test variables were positively associated with peak isokinetic knee torque. CONCLUSION: The aerobic performance of physically active patients with HIV/AIDS is generally well conserved. Nevertheless, poor muscle strength is observed in some HIV/AIDS patients, which is associated with lower anaerobic power and ((V) over dotO(2peak)), suggesting the possibility of enhancing the aerobic performance of patients with weak muscles through appropriate muscle-strengthening activities.
- Contemporary reflection on the educational levels of high-performance soccer players in Brazil(2023) SANTOS-SILVA, Paulo Roberto; GREVE, Julia Maria D'Andrea; SILVA, Renato Luis da; SPINOLA, Marcelo MesquitaObjective: As the soccer culture in Brazil is more popular than schooling, this study reflected on the formal education levels of soccer (football) players through descriptive and quantitative analyses. Methods: We evaluated 179 national soccer players playing various positions on different teams across six seasons (2012, 2013, 2014, 2015, 2016, and 2022). Data were collected using a questionnaire comprising the following variables: age, position in the pitch, age of professionalization, and education (years of study). The data were distributed according to the pitch position. Results: The mean age, length of professional career, and professionalization age were 23 +/- 6 years, 7 +/- 5 years, and 17 +/- 2 years, respectively. In terms of education, 121 athletes (67%) completed high school, equivalent to 11 years of study. Only 5.5% completed higher education, with defensive players (goalkeepers and defenders) being the most educated at 37% (66/179). According to 2017 figures from the Pesquisa Nacional por Amostra de Domicilios Continua of the Federal Government of Brazil, the schooling rate is 31.7%, wherein 46.1% of Brazilians aged 25 years or over have completed education. In this study of 179 athletes, 67% had completed high school. Thus, players outrank the Brazilian population in terms of achieving a high school education. There were marked disparities in the relative proportions of goalkeepers (85%), defensive players (68%), midfielders (63%), and forward players (64%) in terms of their education. Conclusion: Our survey revealed that attending higher education remains a distant reality in Brazil.