SANIA ALVES DOS SANTOS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
14 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 14
- Prevalence of Clostridioides difficile associated diarrhea in hospitalized patients in five Brazilian centers: A multicenter, prospective study(2020) GIRAO, Evelyne Santana; TAVARES, Bruno de Melo; SANTOS, Sania Alves dos; GAMARRA, Gessica Lorena; RIZEK, Camila; MARTINS, Roberta Cristina; NETO, Lauro Vieira Perdigao; DIOGO, Constancia; ORSI, Tatiana D'Annibale; ESPINOZA, Evelyn Patricia Sanchez; MORALES, Hugo Manuel Paz; NOGUEIRA, Keite da Silva; MAESTRI, Adriane Ceshin; BOSZCZOWSKI, Icaro; PIASTRELLI, Filipe; COSTA, Cecilia Leite; COSTA, Daniely Viana; MACIEL, Geovania; ROMAO, Janete; GUIMARAES, Thais; BRITO, Gerly Anne de Castro; COSTA, Silvia FigueiredoEpidemiological data on CD infection (CDI) in Latin American are scarce. CDI prevalence and strains characterization were prospectively evaluated in 5 Brazilian hospitals from different regions. Prevalence rates of CDI were 15%, ranging from 0 to 37%. ST42 was the most common Sequence Type and hyper virulent strains were not identified.
- The changing epidemiology of Acinetobacter spp. producing MA. carbapenemases causing bloodstream infections in Brazil: a BrasNet report(2015) VASCONCELOS, Ana Tereza R.; BARTH, Afonso L.; ZAVASCKI, Alexandre P.; GALES, Ana C.; LEVIN, Anna S.; LUCAREVSCHI, Bianca R.; CABRAL, Blenda G.; BRASILIENSE, Danielle M.; ROSSI, Flavia; FURTADO, Guilherme H. C.; CARNEIRO, Irna Carla R. S.; SILVA, Juliana O. da; RIBEIRO, Julival; LIMA, Karla V. B.; CORREA, Luci; BRITTO, Maria H.; SILVA, Mariama T.; CONCEICAO, Marilia L. da; MOREIRA, Marina; MARTINO, Marines D. V.; FREITAS, Manse R. de; OLIVEIRA, Maura S.; DALBEN, Mirian F.; GUZMAN, Ricardo D.; CAYO, Rodrigo; MORAIS, Rosangela; SANTOS, Sania A.; MARTINS, Willames M. B. S.We evaluated the epidemiology of Acinetobacter spp. recovered from patients diagnosed with bloodstream infections in 9 tertiary hospitals located in all Brazilian geographic regions between April and August 2014. Although OXA-23-producing Acinetobacter baumannii clones were disseminated in most hospitals, it was observed for the first time the spread of OXA-72 among clonally related A. baumannii isolated from distinct hospitals. Interestingly, Acinetobacter pittii was the most frequent species found in a Northern region hospital. Contrasting with the multisusceptible profile displayed by A. pittii isolates, the tetracyclines and polymyxins were the only antimicrobials active against all A. baumannii isolates.
- MRSA outbreak in a Neonatal Intensive Care Unit in a developed country: importance of rapid detection of reservoirs and implementation of intervention measures(2022) MOURA, Maria Luisa; RIZEK, Camila Fonseca; AGUIAR, Elisa; BARROS, Ana Natiele da Silva; COSTA, Sibeli; SANTOS, Sania Alves dos; MARCHI, Ana Paula; GIBELLI, Maria Augusta Bento Cicaroni; TRAGANTE, Carla Regina; ARAUJO, Maria Rita Elmor de; ROSSI, Flavia; GUIMARAES, Thais; COSTA, Silvia FigueiredoWe described a MRSA bloodstream infection outbreak that was rapidly identified and controlled in a Neonatal Intensive Care Unit after implementation of a bundle of measures, including PCR-screening and HCW decolonization. We found 35% of healthcare workers(HCW) colonized with S. aureus by PCR, one of them that presented skin lesion positive for MSSA (same clone and spa type than two patients). Our findings raise the hypothesis that the outbreak could be related to HCW colonization.
- Risk factors for bloodstream infection by multidrug-resistant organisms in critically ill patients in a reference trauma hospital(2022) CAMPOS, Luciana Rodrigues Pires de; CORTES, Marina Farrel; DEO, Beatriz; RIZEK, Camila; SANTOS, Sania; PERDIGAO, Lauro; COSTA, Silvia FigueiredoBackground: Bloodstream infections (BSI) by multidrug-resistant (MDR) organisms are responsible for significant mortality in critically ill trauma patients. Our objective is to identify the risk factors for BSI by MDR agents and their resistance mechanisms in a trauma reference hospital. Methods: During 18 months, all patients admitted in our Intensive Care Unit (ICU) were enrolled in this prospective cohort. We included the first episode of BSI by carbapenem-resistant Gram-negative bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococcus. Demographic and clinical data were compared among patients with and without BSI and variables with P <.05 were tested in a multivariate analysis. We performed PCR for identification of carbapenemase and SCC mec genes and Pulsed-field gel electrophoresis for clonality. Results: Out of 1,528 patients, 302 (19.8%) were trauma and 66 (4.3%) had a MDR-BSI ( 19.5% were trauma). The multivariate analysis showed that mechanical ventilation (OR3.16; 95% CI 1-8; P =.02), hemodialysis (OR3.16; 95% CI 1-5; P =.0003) and surgery (OR1.76; 95% CI 1-3; P =.04) were independent risk factors for MDR- BSI. The most frequent MDR were Klebsiella pneumoniae (n = 26) and MRSA (n = 27). Regarding K pneumoniae strains (n = 24), 20 ( 83.8%) harbored bla KPC gene and 1 bla NDM. The majority of KPC isolates belonged to a predominant clone; while the MRSA were polyclonal and SCC mec type II. Conclusions: Mechanical ventilation, surgery and hemodialysis were independent risk factors for MDR-BSI in our cohort, but trauma was not. KPC was the main mechanism of resistance among carbapenem-resistant K pneumoniae that belonged to a predominant clone which could indicate cross-transmission.
- Evaluation of loop-mediated isothermal amplification assay for detection of Clostridioides difficile infection: A prospective diagnostic performance study(2021) GIRA, Evelyne Santana; NOGUERA, Saidy Liceth Vasconez; ESPINOZA, Evelyn Patricia Sanchez; BEZERRA, Camila Loredana Pereira Alves Madeira; LINO, Fernando Molina; CORTES, Marina Farrel; SANTOS, Sania Alves dos; PARANHOS-BACCALA, Glaucia; PERDIGA, Lauro Vieira; COSTA, Silvia FigueiredoA novel Loop-mediated isothermal amplification (LAMP) assay, HiberGene's CD was evaluated with 82 unformed stools from patients suspected of C. difficile infection (CDI). Compared to glutamate dehy-drogenase (GDH) toxins A/B test (C.diff Quik Chek (R)), HiberGene's LAMP showed 100% of sensitivity and 95,8% of specificity; and compared to FilmArrayTM GI panel (R) (BioFire), a sensitivity of 81,2% and a specificity of 100%, with 96.38% of agreement.
- Quantification of the Expression of HIF-1alpha by Real-Time PCR in Rat Hepatocytes Cultures Invaded by Shigella flexneri under Normoxic and Hypoxic Conditions(2015) LIMA, Camila Barbara Cantalupo; SANTOS, Sania Alves dos; ANDRADE JUNIOR, Dahir Ramos deAim: Shigella flexneri (S. flexneri) is a gram-negative enterobacterium responsible for severe intestinal end systemic infection in humans. The bacteria can reach the liver due to degeneration of the colonic epithelium. Hypoxia is present in many human diseases and can induce the expression of the transcription factor HIF-1alpha that may have a cell protective role. The influence of hypoxia and HIF-1alpha on bacterial infection, studied in this work, is unclear. Hypoxia inducible factor-1alpha (HIF-1alpha) is a transcription factor that acts as a master regulator of gene expression induced by hypoxia. Methods: We compared the ability of S. flexneri to invade rat hepatocytes in primary culture both in normoxic and hypoxic conditions. We evaluated TNF-alpha released by hepatocytes, apoptosis rate and HIF-1alpha expression by confocal microscopy as well as real time PCR technique. Results: We showed that S. flexneri invaded less hepatocytes previously submitted to 24 h hypoxia (6.5% O-2) than those cultivated in normoxia (21% O-2). S. flexneri also induced HIF-1 alpha expression in hepatocytes, TNF-alpha secretion and apoptosis. Conclusion: a) Hypoxia alone was not a stimulus to TNF-alpha secretion, but induced cell apoptosis and HIF-1 alpha expression; b) S. flexneri was able to invade rat hepatocytes and hypoxia apparently influenced significantly bacterial cell invasiveness; c) HIF-1 alpha was expressed in hypoxic conditions, and it was also stimulated by S. flexneri.
- A Model-Based Strategy to Control the Spread of Carbapenem-Resistant Enterobacteriaceae: Simulate and Implement(2016) DALBEN, Mirian de Freitas; MENDES, Elisa Teixeira; MOURA, Maria Luisa; RAHMAN, Dania Abdel; PEIXOTO, Driele; SANTOS, Sania Alves dos; FIGUEIREDO, Walquiria Barcelos de; MENDES, Pedro Vitale; TANIGUCHI, Leandro Utino; COUTINHO, Francisco Antonio Bezerra; MASSAD, Eduardo; LEVIN, Anna SaraOBJECTIVE. To reduce transmission of carbapenem-resistant Enterobacteriaceae (CRE) in an intensive care unit with interventions based on simulations by a developed mathematical model. DESIGN. Before-after trial with a 44-week baseline period and 24-week intervention period. SETTING. Medical intensive care unit of a tertiary care teaching hospital. PARTICIPANTS. All patients admitted to the unit. METHODS. We developed a model of transmission of CRE in an intensive care unit and measured all necessary parameters for the model input. Goals of compliance with hand hygiene and with isolation precautions were established on the basis of the simulations and an intervention was focused on reaching those metrics as goals. Weekly auditing and giving feedback were conducted. RESULTS. The goals for compliance with hand hygiene and contact precautions were reached on the third week of the intervention period. During the baseline period, the calculated R0 was 11; the median prevalence of patients colonized by CRE in the unit was 33%, and 3 times it exceeded 50%. In the intervention period, the median prevalence of colonized CRE patients went to 21%, with a median weekly Rn of 0.42 (range, 0-2.1). CONCLUSIONS. The simulations helped establish and achieve specific goals to control the high prevalence rates of CRE and reduce CRE transmission within the unit. The model was able to predict the observed outcomes. To our knowledge, this is the first study in infection control to measure most variables of a model in real life and to apply the model as a decision support tool for intervention.
- TNF-alpha PRODUCTION AND APOPTOSIS IN HEPATOCYTES AFTER Listeria monocytogenes AND Salmonella TYPHIMURIUM INVASION(2011) SANTOS, Sania Alves dos; ANDRADE JUNIOR, Dahir Ramos de; ANDRADE, Dahir Ramos deInvasion of hepatocytes by Listeria monocytogenes (LM) and Salmonella Typhimurium (ST) can stimulate tumor necrosis factor alpha (TNF-alpha) release and induce apoptosis. In this study, we compared the behavior of hepatocytes invaded by three L. monocytogenes serotypes (LM-4a, LM-4b and LM-1/2a) and by ST to understand which bacterium is more effective in the infectious process. We quantified INF-alpha release by ELISA, apoptosis rates by annexin V (early apoptosis) and TUNEL (late apoptosis) techniques. The cell morphology was studied too. TNF-alpha release rate was highest in ST-invaded hepatocytes. ST and LM-1/2a induced the highest apoptosis production rates evaluated by TUNEL. LM-4b produced the highest apoptosis rate measured by annexin. Invaded hepatocytes presented various morphological alterations. Overall, LM-4b and LM-1/2a proved to be the most efficient at cell invasion, although ST adapted faster to the environment and induced earlier hepatocyte TNF-alpha release.
- Healthcare Professionals Perception of Mobile Phone Usage and Hand Hygiene Adhesion in Intensive Care Units(2020) SANCHEZ, Evelyn; PERDIGAO-NETO, Lauro; SANTOS, Sania Alves dos; RIZEK, Camila; GOMEZ, Maria Renata; MARTINS, Roberta; OLIVEIRA, Gaspar de; GUIMARAES, Thais; BOSZCZOWSKI, Icaro; ROSSI, Flavia; FREIRE, Maristela; LEVIN, Anna; COSTA, Silvia Figueiredo; FARREL, Marina
- Staphylococcus spp Resistance to Chlorhexidine: Is There Any Impact Related to the Routine Use for Hand Hygiene?(2020) BOSZCZOWSKI, Icaro; MINAMI, William Kazumassa; BARALDI, Marcia; MARCHI, Ana Paula; SANTOS, Sania Alves dos; SCHMITT, Cristiane; MACIEL, Amanda Luiz Pires; ZANI, Maria Eduarda Rufino; SOUZA, Leticia Muniz; SOUZA, Nicole Soares de; COSTA, Silvia Figueiredo