MARA DE SOUZA JUNQUEIRA

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Lattes
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Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina

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  • article 9 Citação(ões) na Scopus
    Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic-Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model
    (2021) OLIVEIRA, Fernando A.; NUCCI, Mariana P.; MAMANI, Javier B.; ALVES, Arielly H.; REGO, Gabriel N. A.; KONDO, Andrea T.; HAMERSCHLAK, Nelson; JUNQUEIRA, Mara S.; SOUZA, Lucas E. B. de; GAMARRA, Lionel E.
    This study proposes an innovative way to evaluate the homing and tracking of hematopoietic stem cells from young and old mice labeled with SPIONNIRF-Rh conjugated with two types of fluorophores (NIRF and Rhodamine), and their grafting by bioluminescence (BLI) in a bone marrow transplant (BMT) model. In an in vitro study, we isolated bone marrow mononuclear cells (BM-MNC) from young and old mice, and analyzed the physical-chemical characteristics of SPIONNIRF-Rh, their internalization, cell viability, and the iron quantification by NIRF, ICP-MS, and MRI. The in vivo study was performed in a BMT model to evaluate the homing, tracking, and grafting of young and old BM-MNC labeled with SPIONNIRF-Rh by NIRF and BLI, as well as the hematological reconstitution for 120 days. 5FU influenced the number of cells isolated mainly in young cells. SPIONNIRF-Rh had adequate characteristics for efficient internalization into BM-MNC. The iron load quantification by NIRF, ICP-MS, and MRI was in the order of 10(4) SPIONNIRF-Rh/BM-MNC. In the in vivo study, the acute NIRF evaluation showed higher signal intensity in the spinal cord and abdominal region, and the BLI evaluation allowed follow-up (11-120 days), achieving a peak of intensity at 30 days, which remained stable around 10(8) photons/s until the end. The hematologic evaluation showed similar behavior until 30 days and the histological results confirm that iron is present in almost all tissue evaluated. Our results on BM-MNC homing and tracking in the BMT model did not show a difference in migration or grafting of cells from young or old mice, with the hemogram analysis trending to differentiation towards the myeloid lineage in mice that received cells from old animals. The cell homing by NIRF and long term cell follow-up by BLI highlighted the relevance of the multimodal nanoparticles and combined techniques for evaluation.
  • article 16 Citação(ões) na Scopus
    The Promigratory Activity of the Matricellular Protein Galectin-3 Depends on the Activation of PI-3 Kinase
    (2011) MELO, Fabiana H. M.; BUTERA, Diego; JUNQUEIRA, Mara de Souza; HSU, Daniel K.; SILVA, Ana Maria Moura da; LIU, Fu-Tong; SANTOS, Marinilice F.; CHAMMAS, Roger
    Expression of galectin-3 is associated with sarcoma progression, invasion and metastasis. Here we determined the role of extracellular galectin-3 on migration of sarcoma cells on laminin-111. Cell lines from methylcholanthrene-induced sarcomas from both wild type and galectin-3(-/-) mice were established. Despite the presence of similar levels of laminin-binding integrins on the cell surface, galectin-3(-/-) sarcoma cells were more adherent and less migratory than galectin-3+/+ sarcoma cells on laminin-111. When galectin-3 was transiently expressed in galectin-3(-/-) sarcoma cells, it inhibited cell adhesion and stimulated the migratory response to laminin in a carbohydrate-dependent manner. Extracellular galectin-3 led to the recruitment of SHP-2 phosphatase to focal adhesion plaques, followed by a decrease in the amount of phosphorylated FAK and phospho-paxillin in the lamellipodia of migrating cells. The promigratory activity of extracellular galectin-3 was inhibitable by wortmannin, implicating the activation of a PI-3 kinase dependent pathway in the galectin-3 triggered disruption of adhesion plaques, leading to sarcoma cell migration on laminin-111.
  • article 37 Citação(ões) na Scopus
    O-glycan sialylation alters galectin-3 subcellular localization and decreases chemotherapy sensitivity in gastric cancer
    (2016) SANTOS, Sofia N.; JUNQUEIRA, Mara S.; FRANCISCO, Guilherme; VILANOVA, Manuel; MAGALHAES, Ana; BARUFFI, Marcelo Dias; CHAMMAS, Roger; HARRIS, Adrian L.; REIS, Celso A.; BERNARDES, Emerson S.
    ST6GalNAc-I, the sialyltransferase responsible for sialyl-Tn (sTn) synthesis, has been previously reported to be positively associated with cancer aggressiveness. Here we describe a novel sTn-dependent mechanism for chemotherapeutic resistance. We show that sTn protects cancer cells against chemotherapeutic-induced cell death by decreasing the interaction of cell surface glycan receptors with galectin-3 and increasing its intracellular accumulation. Moreover, exogenously added galectin-3 potentiated the chemotherapeutics-induced cytotoxicity in sTn non-expressing cells, while sTn overexpressing cells were protected. We also found that the expression of sTn was associated with a reduction in galectin-3-binding sites in human gastric samples tumors. ST6GalNAc-I knockdown restored galectin-3-binding sites on the cell surface and chemotherapeutics sensibility. Our results clearly demonstrate that an interruption of O-glycans extension caused by ST6GalNAc-I enzymatic activity leads to tumor cells resistance to chemotherapeutic drugs, highlighting the need for the development of novel strategies to target galectin-3 and/or ST6GalNAc-I.
  • conferenceObject
    Near Infrared Fluorescence In-Vivo Imaging of Non-Hodgkin Lymphoma Using Cy7-Bevacizumab
    (2017) CAMACHO, Ximena; PERRONI, Carolina; JUNQUEIRA, Mara de Souza; FERNANDEZ, Marcelo; BUSTOS, Silvina; BUCHPIGUEL, Carlos; CHAMMAS, Roger; GAMBINI, Juan Pablo; CABRAL, Pablo; RIVA, Eloisa
  • article 1 Citação(ões) na Scopus
    Potential of [C-11](R)-PK11195 PET Imaging for Evaluating Tumor Inflammation: A Murine Mammary Tumor Model
    (2022) SOUZA, Aline Morais de; REAL, Caroline Cristiano; JUNQUEIRA, Mara de Souza; SOUZA, Larissa Estessi de; MARQUES, Fabio Luiz Navarro; BUCHPIGUEL, Carlos Alberto; CHAMMAS, Roger; SAPIENZA, Marcelo Tatit; FARIA, Daniele de Paula
    Background: Breast tumor inflammation is an immunological process that occurs mainly by mediation of Tumor-Associated Macrophages (TAM). Aiming for a specific measurement of tumor inflammation, the current study evaluated the potential of Positron Emission Tomography (PET) imaging with [C-11](R)-PK11195 to evaluate tumor inflammation in a mammary tumor animal model. Methods: Female Balb/C mice were inoculated with 4T1 cells. The PET imaging with [C-11](R)-PK11195 and [F-18]FDG was acquired 3 days, 1 week, and 2 weeks after cell inoculation. Results: The [C-11](R)-PK11195 tumor uptake increased from 3 days to 1 week, and decreased at 2 weeks after cell inoculation, as opposed to the [F-18]FDG uptake, which showed a slight decrease in uptake at 1 week and increased uptake at 2 weeks. In the control group, no significant differences occurred in tracer uptake over time. Tumor uptake of both radiopharmaceuticals is more expressed in tumor edge regions, with greater intensity at 2 weeks, as demonstrated by [C-11](R)-PK11195 autoradiography and immunofluorescence with TSPO antibodies and CD86 pro-inflammatory phenotype. Conclusion: The [C-11](R)-PK11195 was able to identify heterogeneous tumor inflammation in a murine model of breast cancer and the uptake varied according to tumor size. Together with the glycolytic marker [F-18]FDG, molecular imaging with [C-11](R)-PK11195 may provide a better characterization of inflammatory responses in cancer.
  • article 19 Citação(ões) na Scopus
    Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?
    (2022) FERNANDES, Bianca H. Ventura; FEITOSA, Natalia Martins; BARBOSA, Ana Paula; BOMFIM, Camila Gasque; GARNIQUE, Anali M. B.; ROSA, Ivana F.; RODRIGUES, Maira S.; DORETTO, Lucas B.; COSTA, Daniel F.; CAMARGO-DOS-SANTOS, Bruno; FRANCO, Gabrielli A.; FAVERO NETO, Joao; LUNARDI, Juliana Sartori; BELLOT, Marina Sanson; ALVES, Nina Pacheco Capelini; COSTA, Camila C.; ARACATI, Mayumi F.; RODRIGUES, Leticia F.; CIRILO, Rafaela Hemily; COLAGRANDE, Raul Marcelino; GOMES, Francisco I. F.; NAKAJIMA, Rafael T.; BELO, Marco A. A.; GIAQUINTO, Percilia Cardoso; OLIVEIRA, Susana Luporini de; ETO, Silas Fernandes; FERNANDES, Dayanne Carla; MANRIQUE, Wilson G.; CONDE, Gabriel; ROSALES, Roberta R. C.; TODESCHINI, Iris; RIVERO, Ilo; LLONTOP, Edgar; SGRO, German G.; OKA, Gabriel Umaji; BUENO, Natalia Fernanda; FERRARIS, Fausto K.; MAGALHAES, Mariana T. Q. de; MEDEIROS, Renata J.; MENDONCA-GOMES, Juliana M.; JUNQUEIRA, Mara Souza; CONCEICAO, Katia; PONTES, Leticia Gomes de; CONDINO-NETO, Antonio; PEREZ, Andrea C.; BARCELLOS, Leonardo J. G.; CORREA JUNIOR, Jose Dias; DORLASS, Erick Gustavo; CAMARA, Niels O. S.; DURIGON, Edison Luiz; CUNHA, Fernando Q.; NOBREGA, Rafael H.; MACHADO-SANTELLI, Glaucia M.; FARAH, Chuck S.; VERAS, Flavio P.; GALINDO-VILLEGAS, Jorge; V, Leticia Costa-Lotufo; CUNHA, Thiago M.; CHAMMAS, Roger; CARVALHO, Luciani R.; GUZZO, Cristiane R.; MALAFAIA, Guilherme; CHARLIE-SILVA, Ives
    Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
  • conferenceObject
    Radioactive and near-infrared fluorescence in vivo imaging of Non-Hodgkin Lymphoma using 99mTc/Cy7-Fab(Bevacizumab)
    (2021) CAMACHO, X.; PERRONI, C.; CARNEIRO, C.; JUNQUEIRA, M.; FARIA, D.; GARCIA, M.; FERNANDEZ, M.; BUCHPIGUEL, C.; CERECETTO, H.; CHAMMAS, R.; RIVA, E.; CABRAL, P.; GAMBINI, J.
  • article 0 Citação(ões) na Scopus
    Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation
    (2023) GARRIGOS, Murilo M.; OLIVEIRA, Fernando A.; NUCCI, Mariana P.; MAMANI, Javier B.; DIAS, Olivia F. M.; REGO, Gabriel N. A.; JUNQUEIRA, Mara S.; COSTA, Cicero J. S.; SILVA, Lucas R. R.; ALVES, Arielly H.; VALLE, Nicole M. E.; MARTI, Luciana; GAMARRA, Lionel F.
    Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 mu g Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 +/- 0.37 mu g Fe/mL in the control and 6.61 +/- 0.84 mu g Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 +/- 0.66 mg Fe/g in the spleen of the control group and 2.17 +/- 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness.
  • article 10 Citação(ões) na Scopus
    Enriched inorganic compounds in diesel exhaust particles induce mitogen-activated protein kinase activation, cytoskeleton instability, and cytotoxicity in human bronchial epithelial cells
    (2015) SERIANI, Robson; JUNQUEIRA, Mara S.; CARVALHO-SOUSA, Claudia E.; ARRUDA, Alessandra Ct.; MARTINEZ, Diana; ALENCAR, Adriano M.; GARIPPO, Ana L.; BRITO, Jose Mara; MARTINS, Milton A.; SALDIVA, Paulo H. N.; NEGRI, Elnara M.; MAUAD, Thais; MACCHIONE, Mariangela
    This study assessed the effects of the diesel exhaust particles on ERR and JNK MAPKs activation, cell rheology (viscoelasticity), and cytotoxicity in bronchial epithelial airway cells (BEAS-2B). Crude DEP and DEP after extraction with hexane (DEP/HEX) were utilized. The partial reduction of some DEP/HEX organics increased the biodisponibility of many metallic elements. JNK and ERR were activated simultaneously by crude DEP with no alterations in viscoelasticity of the cells. Mitochondrial activity, however, revealed a decrease through the MIT assay. DEP/HEX treatment increased viscoelasticity and cytotoxicity (membrane damage), and also activated JNK. Our data suggest that the greater bioavailability of metals could be involved in JNK activation and, consequently, in the reduction of fiber coherence and increase in the viscoelasticity and cytotoxicity of BEAS cells. The adverse findings detected after exposure to crude DEP and to DEP/HEX reflect the toxic potential of diesel compounds. Considering the fact that the cells of the respiratory epithelium are the first line of defense between the body and the environment, our data contribute to a better understanding of the pathways leading to respiratory cell injury and provide evidence for the onset of or worsening of respiratory diseases caused by inorganic compounds present in DEP.
  • article 1 Citação(ões) na Scopus
    Radio- and Fluorescent-Labeling of Rituximab Based on the Inverse Electron Demand Diels-Alder Reaction
    (2021) GARCIA, Maria Fernanda; JUNQUEIRA, Mara de Souza; MORORO, Janio da Silva; CAMACHO, Ximena; FARIA, Daniele de Paula; CARNEIRO, Camila de Godoi; GALLAZZI, Fabio; CHAMMAS, Roger; QUINN, Thomas; CABRAL, Pablo; CERECETTO, Hugo
    The bioorthogonal reaction between([1,2,4,5])tetrazines with trans-cyclooctene through the inverse electron demand Diels-Alder (IEDDA) has been described as powerful bioconjugation tool. In this work, we explore the IEDDA as a modular conjugation strategy for in vitro and in vivo labeling of Rituximab for the generation of radioactive and fluorescently label immunoconjugates. The strategy allowed the generation, in vitro and in vivo, of conjugated Rituximab with cyanine 5 and 7 and the gamma emmiter technetium-99m.