PETRA DE MELLO MOTTA ARANTES
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
8 resultados
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conferenceObject Aerobic Exercise Attenuates Skeletal Muscle Injury Induced By Cigarette Smoke Exposure In Mice(2014) TOLEDO-ARRUDA, A. C.; SUEHIRO, C. L.; GUARNIER, F. A.; VIEIRA, R. D. P.; ALMEIDA, F. M.; LOPES, F. D.; ARANTES, P. D. M. M.; CECCHINI, R.; LIN, C. J.; MARTINS, M. D. A.- Time-course effects of aerobic physical training in the prevention of cigarette smoke-induced COPD(2017) TOLEDO-ARRUDA, Alessandra C.; VIEIRA, Rodolfo P.; GUARNIER, Flavia A.; SUEHIRO, Camila L.; CALEMAN-NETO, Agostinho; OLIVO, Clarice R.; ARANTES, Petra M. M.; ALMEIDA, Francine M.; LOPES, Fernanda D. T. Q. S.; RAMOS, Ercy M. C.; CECCHINI, Rubens; LIN, Chin Jia; MARTINS, Milton ArrudaA previous study by our group showed that regular exercise training (ET) attenuated pulmonary injury in an experimental model of chronic exposure to cigarette smoke (CS) in mice, but the time-course effects of the mechanisms involved in this protection remain poorly understood. We evaluated the temporal effects of regular ET in an experimental model of chronic CS exposure. Male C57BL/6 mice were divided into four groups: Control (sedentary + air), Exercise (aerobic training + air), Smoke (sedentary + smoke), and Smoke + Exercise (aerobic training + smoke). Mice were exposed to CS and ET for 4, 8, or 12 wk. Exercise protected mice exposed to CS from emphysema and reductions in tissue damping and tissue elastance after 12 wk (P < 0.01). The total number of inflammatory cells in the bronchoalveolar lavage increased in the Smoke group, mainly due to the recruitment of macrophages after 4 wk, neutrophils and lymphocytes after 8 wk, and lymphocytes and macrophages after 12 wk (P < 0.01). Exercise attenuated this increase in mice exposed to CS. The protection conferred by exercise was mainly observed after exercise adaptation. Exercise increased IL-6 and IL-10 in the quadriceps and lungs (P < 0.05) after 12 wk. Total antioxidant capacity and SOD was increased and TNF-alpha and oxidants decreased in lungs of mice exposed to CS after 12 wk (P < 0.05). The protective effects of exercise against lung injury induced by cigarette smoke exposure suggests that anti-inflammatory mediators and antioxidant enzymes play important roles in chronic obstructive pulmonary disease development mainly after the exercise adaptation. NEW & NOTEWORTHY These experiments investigated for the first time the temporal effects of regular moderate exercise training in cigarette smoke-induced chronic obstructive pulmonary disease. We demonstrate that aerobic conditioning had a protective effect in emphysema development induced by cigarette smoke exposure. This effect was most likely secondary to an effect of exercise on oxidant-antioxidant balance and anti-inflammatory mediators.
- A Treatment with a Protease Inhibitor Recombinant from the Cattle Tick (Rhipicephalus Boophilus microplus) Ameliorates Emphysema in Mice(2014) LOURENCO, Juliana D.; NEVES, Luana P.; OLIVO, Clarice R.; DURAN, Adriana; ALMEIDA, Francine M.; ARANTES, Petra M. M.; PRADO, Carla M.; LEICK, Edna Aparecida; TANAKA, Aparecida S.; MARTINS, Milton A.; SASAKI, Sergio D.; LOPES, Fernanda D. T. Q. S.Aims: To determine whether a serine protease inhibitor treatment can prevent or minimize emphysema in mice. Methods: C57BL/6 mice were subjected to porcine pancreatic elastase (PPE) nasal instillation to induce emphysema and were treated with a serine protease inhibitor (rBmTI-A) before (Protocol 1) and after (Protocol 2) emphysema development. In both protocols, we evaluated lung function to evaluate the airway resistance (Raw), tissue damping (Gtis) and tissue elastance (Htis). The inflammatory profile was analyzed in the bronchoalveolar lavage (BALF) and through the use of morphometry; we measured the mean linear intercept (Lm) (to verify alveolar enlargement), the volume proportion of collagen and elastic fibers, and the numbers of macrophages and metalloprotease 12 (MMP-12) positive cells in the parenchyma. We showed that at both time points, even after the emphysema was established, the rBmTI-A treatment was sufficient to reverse the loss of elastic recoil measured by Htis, the alveolar enlargement and the increase in the total number of cells in the BALF, with a primary decrease in the number of macrophages. Although, the treatment did not control the increase in macrophages in the lung parenchyma, it was sufficient to decrease the number of positive cells for MMP-12 and reduce the volume of collagen fibers, which was increased in PPE groups. These findings attest to the importance of MMP-12 in PPE-induced emphysema and suggest that this metalloprotease could be an effective therapeutic target.
conferenceObject A protease inhibitor from the tick rhipicephalus boophilus microplus attenuated parenchymal destruction in elastase-induced emphysema(2013) OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; ALMEIDA, Francine M.; NEVES, Luana Paiva; ARANTES, Petra Mello; PRADO, Carla Maximo; TIBERIO, Iolanda; TANAKA, Aparecida S.; SASAKI, Sergio D.; MARTINS, Milton Arruda; LOPES, Fernanda D. T. Q. S.conferenceObject Cigarette Smoke Associated To Diesel Exhaust Particles (dep) In Mice: Synergy Effects(2013) ARANTES, P. M. M.; TOLEDO, A. C.; ALMEIDA, F. M.; OLIVO, C. R.; MAGALHAES, R. M.; ANG, P. L.; FORNAZIEIRO, L. R.; MACCHIONE, M.; SALDIVA, P. H. N.; BISELLI, P. J. C.; LOPES, F. D. T. Q. S.; MARTINS, M. A.conferenceObject Effects of moderate aerobic exercise trainning in acute and late phase of lung infection induced by streptococcus pneumoniae(2013) OLIVO, Clarice Rosa; MIYAJI, Eliane; OLIVEIRA, Maria Leonor; LOURENCO, Juliana Dias; ALMEIDA, Francine Maria; ARANTES, Petra M.; LOPES, Fernanda D. T. Q. S.; MARTINS, Milton A.- Aerobic exercise attenuates pulmonary inflammation induced by Streptococcus pneumoniae(2014) OLIVO, Clarice R.; MIYAJI, Eliane N.; OLIVEIRA, Maria Leonor S.; ALMEIDA, Francine M.; LOURENCO, Juliana D.; ABREU, Rodrigo M.; ARANTES, Petra M. M.; LOPES, Fernanda D. T. Q. S.; MARTINS, Milton A.Aerobic exercise has been recognized as a stimulator of the immune system, but its effect on bacterial infection has not been extensively evaluated. We studied whether moderate aerobic exercise training prior to Streptococcus pneumoniae infection influences pulmonary inflammatory responses. BALB/c mice were divided into four groups: Sedentary Untreated (sedentary without infection); Sedentary Infected (sedentary with infection); Trained Untreated (aerobic training without infection); and Trained Infected (aerobic training with infection). Animals underwent aerobic training for 4 wk, and 72 h after last exercise training, animals received a challenge with S. pneumoniae and were evaluated either 12 h or 10 days after instillation. In acute phase, Sedentary Infected group had an increase in respiratory system resistance and elastance; number of neutrophils, lymphocytes, and macrophages in bronchoalveolar lavage fluid (BAL); polymorphonuclear cells in lung parenchyma; and levels of keratinocyte-derived chemokine (KC), tumor necrosis factor-alpha (TNF-alpha), and interleukin (IL) - 1 beta (IL-1 beta) in lung homogenates. Exercise training significantly attenuated the increase in all of these parameters and induced an increase in expression of antioxidant enzymes (CuZnSOD and MnSOD) in lungs. Trained Infected mice had a significant decrease in the number of colony-forming units of pneumococci in the lungs compared with Sedentary Infected animals. Ten days after infection, Trained Infected group exhibited lower numbers of macrophages in BAL, polymorphonuclear cells in lung parenchyma and IL-6 in lung homogenates compared with Sedentary Infected group. Our results suggest a protective effect of moderate exercise training against respiratory infection with S. pneumoniae. This effect is most likely secondary to an effect of exercise on oxidant-antioxidant balance.
conferenceObject An experimental model of elastase and cigarette smoke- induced emphysema in mice(2014) RODRIGUES, Rubia; RIANE, Alyne; LOURENCO, Juliana; OLIVO, Clarice; ARANTES, Petra; ALMEIDA, Francine; MARTINS, Milton A.; LOPES, Fernanda D. T. Q. S.