SILVIA YUMI BANDO TAKAHARA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Pediatria, Faculdade de Medicina
LIM/36 - Laboratório de Pediatria Clínica, Hospital das Clínicas, Faculdade de Medicina
LIM/36 - Laboratório de Pediatria Clínica, Hospital das Clínicas, Faculdade de Medicina
36 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 36
- Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus(2020) BERTONHA, Fernanda Bernardi; BANDO, Silvia Yumi; FERREIRA, Leandro Rodrigues; CHACCUR, Paulo; VINHAS, Christiana; ZERBINI, Maria Claudia Nogueira; CARNEIRO-SAMPAIO, Magda Maria; MOREIRA-FILHO, Carlos AlbertoThe human thymus suffers a transient neonatal involution, recovers and then starts a process of decline between the 1st and 2nd years of life. Age-related morphological changes in thymus were extensively investigated, but the genomic mechanisms underlying this process remain largely unknown. Through Weighted Gene Co-expression Network Analysis (WGCNA) and TF-miRNA-mRNA integrative analysis we studied the transcriptome of neonate and infant thymic tissues grouped by age: 0-30 days (A); 31 days-6 months (B); 7-12 months (C); 13-18 months (D); 19-31 months (E). Age-related transcriptional modules, hubs and high gene significance (HGS) genes were identified, as well as TF-miRNA-hub/HGS co-expression correlations. Three transcriptional modules were correlated with A and/or E groups. Hubs were mostly related to cellular/metabolic processes; few were differentially expressed (DE) or related to T-cell development. Inversely, HGS genes in groups A and E were mostly DE. In A (neonate) one third of the hyper-expressed HGS genes were related to T-cell development, against one-twentieth in E, what may correlate with the early neonatal depletion and recovery of thymic T-cell populations. This genomic mechanism is tightly regulated by TF-miRNA-hub/HGS interactions that differentially govern cellular and molecular processes involved in the functioning of the neonate thymus and in the beginning of thymic decline.
- Texture analysis of high resolution MRI allows discrimination between febrile and afebrile initial precipitating injury in mesial temporal sclerosis(2012) ALEGRO, Maryana de Carvalho; SILVA, Alexandre Valotta; BANDO, Silvia Yumi; LOPES, Roseli de Deus; CASTRO, Luiz Henrique Martins de; HUNGTSU, Wen; MOREIRA-FILHO, Carlos Alberto; AMARO JR., EdsonA computational pipeline combining texture analysis and pattern classification algorithms was developed for investigating associations between high-resolution MRI features and histological data. This methodology was tested in the study of dentate gyrus images of sclerotic hippocampi resected from refractory epilepsy patients. Images were acquired using a simple surface coil in a 3.0T MRI scanner. All specimens were subsequently submitted to histological semiquantitative evaluation. The computational pipeline was applied for classifying pixels according to: a) dentate gyrus histological parameters and b) patients' febrile or afebrile initial precipitating insult history. The pipeline results for febrile and afebrile patients achieved 70% classification accuracy, with 78% sensitivity and 80% specificity [area under the reader observer characteristics (ROC) curve: 0.89]. The analysis of the histological data alone was not sufficient to achieve significant power to separate febrile and afebrile groups. Interesting enough, the results from our approach did not show significant correlation with histological parameters (which per se were not enough to classify patient groups). These results showed the potential of adding computational texture analysis together with classification methods for detecting subtle MRI signal differences, a method sufficient to provide good clinical classification. A wide range of applications of this pipeline can also be used in other areas of medical imaging. Magn Reson Med, 2012. (c) 2012 Wiley Periodicals, Inc.
- Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus (vol 15, e0227547, 2020)(2020) BERTONHA, F. B.; BANDO, S. Y.; FERREIRA, L. R.; CHACCUR, P.; VINHAS, C.; ZERBINI, M. C. N.
conferenceObject TRISOMY 21-DRIVEN GENE EXPRESSION DYSREGULATION IN HUMAN THYMUS: CONVERGING GENOMIC AND EPIGENOMIC MECHANISMS(2016) MOREIRA-FILHO, Carlos Alberto; BANDO, Silvia Yumi; BERTONHA, Fernanda Bernardi; SILVA, Filipi Nascimento; COSTA, Luciano da Fontoura; FEREIRA, Leandro Rodrigues; CARNEIRO-SAMPAIO, Magda- Enteroaggregative Escherichia coli with uropathogenic characteristics are present in feces of diarrheic and healthy children(2017) NUNES, Kamila O.; SANTOS, Adriana C. P.; BANDO, Silvia Y.; SILVA, Rosa M.; GOMES, Tania A. T.; ELIAS, Waldir P.Enteroaggregative Escherichia coli (EAEC) has been recently associated with urinary tract infections (UTI). Since EAEC are found in feces of both diarrheic and asymptomatic individuals, their presence in the intestine may be a source of UTI. In this study, we detected in feces of diarrheic and healthy children a subset of EAEC strains with genetic markers of extraintestinal pathogenic E. coli (ExPEC). MLST grouped these EAEC with ExPEC markers in three main clusters along with prototypes strains of EAEC, uropathogenic E. coli and UTI-causing EAEC. Interestingly, the latter cluster was composed by EAEC with ExPEC markers belonging to phylogroup A and closely related to the uropathogenic EAEC O78:H10 strain. Such attributes suggest that these strains have uropathogenic abilities. Therefore, intestinal carriers of these strains are potentially in risk to develop UTIs.
- Hippocampal CA3 transcriptional modules associated with granule cell alterations and cognitive impairment in refractory mesial temporal lobe epilepsy patients(2021) BANDO, Silvia Yumi; BERTONHA, Fernanda Bernardi; PIMENTEL-SILVA, Luciana Ramalho; OLIVEIRA, Joao Gabriel Mansano de; CARNEIRO, Marco Antonio Duarte; OKU, Mariana Hiromi Manoel; WEN, Hung-Tzu; CASTRO, Luiz Henrique Martins; MOREIRA-FILHO, Carlos AlbertoIn about a third of the patients with epilepsy the seizures are not drug-controlled. The current limitation of the antiepileptic drug therapy derives from an insufficient understanding of epilepsy pathophysiology. In order to overcome this situation, it is necessary to consider epilepsy as a disturbed network of interactions, instead of just looking for changes in single molecular components. Here, we studied CA3 transcriptional signatures and dentate gyrus histopathologic alterations in hippocampal explants surgically obtained from 57 RMTLE patients submitted to corticoamygdalohippocampectomy. By adopting a systems biology approach, integrating clinical, histopathological, and transcriptomic data (weighted gene co-expression network analysis), we were able to identify transcriptional modules highly correlated with age of disease onset, cognitive dysfunctions, and granule cell alterations. The enrichment analysis of transcriptional modules and the functional characterization of the highly connected genes in each trait-correlated module allowed us to unveil the modules' main biological functions, paving the way for further investigations on their roles in RMTLE pathophysiology. Moreover, we found 15 genes with high gene significance values which have the potential to become novel biomarkers and/or therapeutic targets in RMTLE.
conferenceObject Association of Hippocampal CA3 Transcriptional Modules with Language Impairment in Mesial Temporal Lobe Epilepsy(2017) MANSANO-OLIVEIRA, Joao; BANDO, Silvia; BERTONHA, Fernanda; CASTRO, Bettina; MESSAS, Cristiane; WEN, Hung-Tzu; MOREIRA-FILHO, Carlos; CASTRO, Luiz- Panton-Valentine Positive Staphylococcus aureus in Community-Acquired and Hospital-Acquired Pediatric Infections(2019) PEREIRA, Maria Fernanda Badue; BANDO, Silvia Yumi; SASAGAWA, Suzethe Matiko; SILVA, Cely Barreto da; MIMICA, Marcelo Jenne; BEREZIN, Eitan NaamanFrom July 2009 to July 2015, Staphylococcus aureus isolated from pediatric sterile sites were selected. Polymerase chain reaction was used to detect mecA and lukS-PV/lukF-PV genes. The rate of methicillin-resistant Staphylococcus aureus was 37.7%. Ten isolates had the lukS-PV/lukF-PV genes, 2 of which were methicillin-resistant Staphylococcus aureus. Skin and soft tissues infections were significantly associated with lukS-PV/lukF-PV positive isolates, P = 0.008.
- Methods for Gene Co-expression Network Visualization and Analysis(2022) MOREIRA-FILHO, C. A.; BANDO, S. Y.; BERTONHA, F. B.; SILVA, F. N.; COSTA, L. D. F.Gene network analysis is an important tool for studying the changes in steady states that characterize cell functional properties, the genome-environment interplay, and the health-disease transitions. Moreover, gene co-expression and protein–protein interaction (PPI) data can be integrated with clinical, histopathological, and imaging information – a current practice in systems biology – leading, for instance, to the identification of unique and common drivers for disease conditions. In this chapter the fundamentals for gene co-expression network construction, visualization, and analysis are revised, emphasizing its scale-free nature, the measures that express its most relevant topological features, and methods for network validation. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2014, 2022.
conferenceObject MOLECULAR CHARACTERIZATION OF COMPLEMENT C1Q, C2, AND C4 GENES IN JUVENILE SYSTEMIC LUPUS ERYTHEMATOSUS(2013) LIPHAUS, B. L.; UMETSU, N.; BANDO, S.; JESUS, A. A.; ANDRADE, L. E. C.; SILVA, C. A.; CARNEIRO-SAMPAIO, M.