Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCUNHA, A. F.
dc.contributor.authorANDRADE, H. M.
dc.contributor.authorSOUZA, F. N.
dc.contributor.authorFIALHO JUNIOR, L. C.
dc.contributor.authorROSA, D. L. S. O.
dc.contributor.authorSANCHEZ, E. M. Ramos
dc.contributor.authorGIDLUND, M.
dc.contributor.authorGOTO, H.
dc.contributor.authorBRITO, M. A. V. P.
dc.contributor.authorGUIMARAES, A. S.
dc.contributor.authorLAGE, A. P.
dc.contributor.authorREIS, L. C.
dc.contributor.authorLIBERA, A. M. M. P. Della
dc.contributor.authorHEINEMANN, M. B.
dc.contributor.authorCERQUEIRA, M. M. O. P.
dc.date.accessioned2020-06-01T14:57:20Z
dc.date.available2020-06-01T14:57:20Z
dc.date.issued2020
dc.description.abstractStaphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit a, succinyl-diaminopimelate desuccinylase, and cysteinyl-tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Minas GeraisMinas Gerais State Research Foundation (FAPEMIG) [APQ-02210-13]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)National Council for Scientific and Technological Development (CNPq) [481950/2013-3]
dc.description.sponsorshipCoordinator for the Improvement of Higher Education Personnel (CAPES)
dc.description.sponsorshipCNPqNational Council for Scientific and Technological Development (CNPq)
dc.description.sponsorshipSao Paulo State Research FoundationFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2014/23189-4]
dc.identifier.citationJOURNAL OF DAIRY SCIENCE, v.103, n.5, p.4588-4605, 2020
dc.identifier.doi10.3168/jds.2019-17084
dc.identifier.eissn1525-3198
dc.identifier.issn0022-0302
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/36045
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INCeng
dc.relation.ispartofJournal of Dairy Science
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright ELSEVIER SCIENCE INCeng
dc.subjectStaphylococcus aureuseng
dc.subjectintramammary infectioneng
dc.subjectserological proteome analysiseng
dc.subjectimmunoproteomicseng
dc.subjectvaccineeng
dc.subject.otherproteome analysiseng
dc.subject.othermultiplex-pcreng
dc.subject.otherproteinseng
dc.subject.otheridentificationeng
dc.subject.otherexpressioneng
dc.subject.otherimpacteng
dc.subject.wosAgriculture, Dairy & Animal Scienceeng
dc.subject.wosFood Science & Technologyeng
dc.titleComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccineeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalCUNHA, A. F.:Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil; Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil
hcfmusp.author.externalANDRADE, H. M.:Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil
hcfmusp.author.externalSOUZA, F. N.:Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil; Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil; Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, BR-05508270 Sao Paulo, Brazil; Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia, Brazil
hcfmusp.author.externalFIALHO JUNIOR, L. C.:Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil
hcfmusp.author.externalROSA, D. L. S. O.:Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil; Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil
hcfmusp.author.externalGIDLUND, M.:Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508900 Sao Paulo, Brazil
hcfmusp.author.externalBRITO, M. A. V. P.:EMBRAPA Gado Leite, Ave Eugenio do Nascimento 610, BR-36038330 Juiz De Fora, Brazil
hcfmusp.author.externalGUIMARAES, A. S.:Univ Fed Lavras, Dept Med Vet, BR-37200000 Lavras, Brazil
hcfmusp.author.externalLAGE, A. P.:Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil
hcfmusp.author.externalLIBERA, A. M. M. P. Della:Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, BR-05508270 Sao Paulo, Brazil
hcfmusp.author.externalHEINEMANN, M. B.:Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, Brazil
hcfmusp.author.externalCERQUEIRA, M. M. O. P.:Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG, Brazil
hcfmusp.citation.scopus12
hcfmusp.contributor.author-fmusphcEDUARDO MILTON RAMOS SANCHEZ
hcfmusp.contributor.author-fmusphcHIRO GOTO
hcfmusp.contributor.author-fmusphcLUIZA DE CAMPOS REIS
hcfmusp.description.beginpage4588
hcfmusp.description.endpage4605
hcfmusp.description.issue5
hcfmusp.description.volume103
hcfmusp.origemWOS
hcfmusp.origem.pubmed32113759
hcfmusp.origem.scopus2-s2.0-85080098208
hcfmusp.origem.wosWOS:000527361400057
hcfmusp.publisher.cityNEW YORKeng
hcfmusp.publisher.countryUSAeng
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