Identification of Candida haemulonii Complex Species: Use of ClinProTools (TM) to Overcome Limitations of the Bruker Biotyper (TM), VITEK MS (TM) IVD, and VITEK MS (TM) RUO Databases

Imagem de Miniatura
Arquivos
art_GRENFELL_Identification_of_Candida_haemulonii_Complex_Species_Use_of_2016.PDF (1.66 MB)
Citações na Scopus
35
Tipo de produção
article
Data de publicação
2016
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Autores
GRENFELL, Rafaella C.
MUNHOZ, Regina B.
ASSIS, Diego M.
ROCKSTROH, Anna C.
JULIANO, Luiz
Citação
FRONTIERS IN MICROBIOLOGY, v.7, article ID 940, 10p, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Candida haemulonli is now considered a complex of two species and one variety: C. haemulonii sensu strict, Candida duobushaemulonii and the variety C. haemulonii var. vulnera. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of C. haemulonli species, analyzing isolates/strains of C. haemulonli complex species, Candida pseudohaemulonii and Candida auris by two commercial platforms, their databases and softwares. To differentiate C. haemulonli sensu sctricto from the variety vulnera, we used the ClinProTools (TM) models and a single-peak analysis with the software FlexAnalysis (TM). The Biotyper (TM) database gave 100% correct species ID for C. haemulonii sensu strict, C. pseudohaemulonii and C. auris, with 69% of correct species ID for C. duobushaemulonii. Vitek MS (TM) IVD database gave 100% correct species ID for C. haemulonii sensu stricto, misidentifying all C. duobushaemulonii and C. pseudohaemulonii as C. haemulonii, being unable to identify C. auris. The Vitek MS (TM) RUO database needed to be upgraded with in-house SuperSpectra to discriminate C. haemulonii sensu stricto, C. duobushaemulonii, C. pseudohaemulonii, and C. auris strains/isolates. The generic algorithm model from ClinProTools (TM) software showed recognition capability of 100% and cross validation of 98.02% for the discrimination of C. haemulonli sensu stricto from the variety vulnera. Single-peak analysis showed that the peaks 5670, 6878, or 13750 m/z can distinguish C. haemulonli sensu stricto from the variety vulnera.
Palavras-chave
MALDI-TOF mass spectrometry, Candida haemulonii complex species, Microflex LT (TM), Vitek MS (TM), ClinProTools (TM)
URI
https://observatorio.fm.usp.br/handle/OPI/16173
Referências
  1. Angeletti S, 2015, NEW MICROBIOL, V38, P541
  2. Bader O, 2013, PROTEOMICS, V13, P788, DOI 10.1002/pmic.201200468
  3. Bittar F, 2009, INT J ANTIMICROB AG, V34, P467, DOI 10.1016/j.ijantimicag.2009.03.017
  4. Branda JA, 2013, J CLIN MICROBIOL, V51, P3079, DOI 10.1128/JCM.00824-13
  5. Brandt M.E., 2012, CURR FUNGAL INFECT R, V6, P170
  6. Cassagne C, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0028425
  7. Cendejas-Bueno E, 2012, J CLIN MICROBIOL, V50, P3641, DOI 10.1128/JCM.02248-12
  8. Chao QT, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0109376
  9. de Almeida JN, 2016, EMERG INFECT DIS, V22, P561, DOI 10.3201/eid2203.151610
  10. de Almeida JN, 2014, CLIN MICROBIOL INFEC, V20, P784, DOI 10.1111/1469-0691.12502
  11. de Almeida JN, 2014, J MICROBIOL METH, V105, P105, DOI 10.1016/j.mimet.2014.07.018
  12. Jamal WY, 2014, INT J INFECT DIS, V26, P167, DOI 10.1016/j.ijid.2014.05.031
  13. Kathuria S, 2015, J CLIN MICROBIOL, V53, P1823, DOI 10.1128/JCM.00367-15
  14. Ketterlinus R., 2005, BIOTECHNIQUES, V38, P37
  15. LEHMANN PF, 1993, J CLIN MICROBIOL, V31, P1683
  16. Luo GZ, 2002, J CLIN MICROBIOL, V40, P2860, DOI 10.1128/JCM.40.8.2860-2865.2002
  17. Merseguel KB, 2015, BMC INFECT DIS, V15, DOI 10.1186/s12879-015-0793-3
  18. Pinto A, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0025712
  19. Quiles-Melero I, 2012, EUR J CLIN MICROBIOL, V31, P67, DOI 10.1007/s10096-011-1277-z
  20. Ramos LS, 2015, J ANTIMICROB CHEMOTH, V70, P111, DOI 10.1093/jac/dku321
  21. Schoch CL, 2012, P NATL ACAD SCI USA, V109, P6241, DOI 10.1073/pnas.1117018109
  22. Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI 10.1093/molbev/mst197
  23. Turner SA, 2014, CSH PERSPECT MED, V4, DOI 10.1101/cshperspect.a019778
  24. Xiao D, 2014, J CLIN MICROBIOL, V52, P3038, DOI 10.1128/JCM.01265-14
  25. Zhang TT, 2015, J MICROBIOL METH, V117, P122, DOI 10.1016/j.mimet.2015.08.002

Coleções
Artigos e Materiais de Revistas Científicas - FM/MDT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/53