Is maintenance of the ileocecal valve important to the intestinal adaptation mechanisms in a weaning rat model of short bowel?

Imagem de Miniatura
Arquivos
art_BARROS_Is_maintenance_of_the_ileocecal_valve_important_to_2018.PDF (1.58 MB)
Citações na Scopus
3
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
ROTONDO, Italo Gerardo
Citação
PEDIATRIC SURGERY INTERNATIONAL, v.34, n.11, p.1215-1224, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Purpose To evaluate the role of maintenance of the ileocecal valve (ICV) in intestinal adaptation mechanisms, in a weaning rat experimental model of short bowel. Methods Forty animals were operated on to produce short bowel syndrome. They were divided into five groups: maintenance (MV) or resection of ICV (RV), kill after 4 days (MV4 and RV4) or 21 days (MV21 and RV21), and a control group (21-day-old rats). Body weights, small bowel and colon lengths and diameters, villus heights, crypt depths, lamina propria and muscle layer thickness, as well as the apoptosis index of villi and crypts and expression of pro-and anti-apoptotic genes, were studied. Results Preservation of the ICV promoted increased weight gain (p = 0.0001) and intestinal villus height after 21 days; crypt depth was higher in comparison to controls. It was verified a higher expression of Ki-67 in bowel villi and crypts (p = 0.018 and p = 0.015, respectively) in RV4 group and a higher expression in bowel villi of MV4 group animals (p = 0.03). The maintenance of ICV promoted late increased expression of the anti-apoptotic gene Bcl-XL in the colon (p = 0.043, p = 0.002, p = 0.01). Conclusion The maintenance of the ICV led to positive changes in this model.
Palavras-chave
Bowel resection, Short bowel syndrome, Intestine, Newborn, Parenteral and enteral nutrition, Rat
URI
https://observatorio.fm.usp.br/handle/OPI/29318
Referências
  1. Coelho MCM, 2007, J PEDIATR SURG, V42, P613, DOI 10.1016/j.jpedsurg.2006.12.011
  2. Braz MJD, 2018, J PEDIATR SURG, V53, P1408, DOI 10.1016/j.jpedsurg.2017.08.012
  3. Demehri FR, 2015, J PEDIATR SURG, V50, P131, DOI 10.1016/j.jpedsurg.2014.10.011
  4. Diamanti A, 2008, J PEDIATR GASTR NUTR, V47, P450, DOI 10.1097/MPG.0b013e31817af979
  5. Diamanti A, 2012, JPEN-PARENTER ENTER, V36, P501, DOI 10.1177/0148607112438719
  6. Fiore NF, 1998, J PEDIATR SURG, V33, P24, DOI 10.1016/S0022-3468(98)90354-2
  7. Gillingham MB, 2000, AM J PHYSIOL-GASTR L, V278, pG700
  8. Goulet O, 2004, J PEDIATR GASTR NUTR, V38, P250, DOI 10.1097/00005176-200403000-00006
  9. GOULET OJ, 1991, J PEDIATR-US, V119, P18, DOI 10.1016/S0022-3476(05)81032-7
  10. Goulet O, 2009, J PEDIATR GASTR NUTR, V48, pS66, DOI 10.1097/MPG.0b013e3181a118ef
  11. Grant D, 2015, AM J TRANSPLANT, V15, P210, DOI 10.1111/ajt.12979
  12. Gutierrez IM, 2011, SEMIN FETAL NEONAT M, V16, P157, DOI 10.1016/j.siny.2011.02.001
  13. Hebiguchi T, 2015, INT J MOL MED, V35, P724, DOI 10.3892/ijmm.2015.2066
  14. Khan FA, 2015, J PEDIATR-US, V167, P29, DOI 10.1016/j.jpeds.2015.03.040
  15. Knott AW, 2003, J PEDIATR SURG, V38, P440, DOI 10.1053/jpsu.2003.50076
  16. Lail SW, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0181453
  17. Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
  18. Longshore S W, 2009, Minerva Pediatr, V61, P239
  19. Martin CA, 2008, J PEDIATR SURG, V43, P1011, DOI 10.1016/j.jpedsurg.2008.02.015
  20. Onishi S, 2016, PEDIATR SURG INT, V32, P1165, DOI 10.1007/s00383-016-3975-1
  21. Perez-Arana G, 2015, HISTOL HISTOPATHOL, V30, P479, DOI 10.14670/HH-30.479
  22. Petit LM, 2016, J PEDIATR GASTR NUTR, V62, P462, DOI 10.1097/MPG.0000000000000980
  23. QUIGLEY EMM, 1984, GASTROENTEROLOGY, V87, P857
  24. Quiros-Tejeira RE, 2004, J PEDIATR-US, V145, P157, DOI 10.1016/j.jpeds.2004.02.030
  25. Roulis M, 2016, DIFFERENTIATION, V92, P116, DOI 10.1016/j.diff.2016.05.002
  26. Sangild PT, 2014, AM J PHYSIOL-GASTR L, V307, pG1147, DOI 10.1152/ajpgi.00088.2014
  27. Schurink M, 2014, TRANSPL P, V46, P2102, DOI 10.1016/j.transproceed.2014.06.030
  28. Spencer AU, 2005, ANN SURG, V242, P403, DOI 10.1097/01.sla.0000179647.24046.03
  29. Stanger JD, 2013, J PEDIATR SURG, V48, P983, DOI 10.1016/j.jpedsurg.2013.02.070
  30. Sukhotnik I, 2018, PEDIATR SURG INT, V34, P217, DOI 10.1007/s00383-017-4192-2
  31. Sukhotnik I, 2016, PEDIATR SURG INT, V32, P169, DOI 10.1007/s00383-015-3829-2
  32. Tannuri ACA, 2007, CLINICS, V62, P757, DOI 10.1590/S1807-59322007000600016
  33. Welters CFM, 2000, J PEDIATR SURG, V35, P20, DOI 10.1016/S0022-3468(00)80069-X
  34. Wester T, 2017, SURGERY, V161, P818, DOI 10.1016/j.surg.2016.09.011
  35. Xu JM, 2008, WORLD J SURG, V32, P1832, DOI 10.1007/s00268-008-9611-z
  36. Yang Q, 2012, PEDIATR RES, V72, P38, DOI 10.1038/pr.2012.41
  37. Yang Q, 2010, J PEDIATR GASTR NUTR, V50, P16, DOI 10.1097/MPG.0b013e3181c2c2af
  38. Yang Y, 2018, BIOSCIENCE REP, V38, DOI 10.1042/BSR20180077

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - HC/ICr
Artigos e Materiais de Revistas Científicas - LIM/07
Artigos e Materiais de Revistas Científicas - LIM/26
Artigos e Materiais de Revistas Científicas - LIM/30