Molecular and Biomechanical Clues From Cardiac Tissue Decellularized Extracellular Matrix Drive Stromal Cell Plasticity

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art_LIGUORI_Molecular_and_Biomechanical_Clues_From_Cardiac_Tissue_Decellularized_2020.PDF (2.36 MB)
Citações na Scopus
34
Tipo de produção
article
Data de publicação
2020
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Autores
TERLIZZI, Vincenzo
DONGEN, Joris A. van
SHARMA, Prashant K.
HARMSEN, Martin Conrad
Citação
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.8, article ID 520, 19p, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Decellularized-organ-derived extracellular matrix (dECM) has been used for many years in tissue engineering and regenerative medicine. The manufacturing of hydrogels from dECM allows to make use of the pro-regenerative properties of the ECM and, simultaneously, to shape the material in any necessary way. The objective of the present project was to investigate differences between cardiovascular tissues (left ventricle, mitral valve, and aorta) with respect to generating dECM hydrogels and their interaction with cells in 2D and 3D. The left ventricle, mitral valve, and aorta of porcine hearts were decellularized using a series of detergent treatments (SDS, Triton-X 100 and deoxycholate). Mass spectrometry-based proteomics yielded the ECM proteins composition of the dECM. The dECM was digested with pepsin and resuspended in PBS (pH 7.4). Upon warming to 37 degrees C, the suspension turns into a gel. Hydrogel stiffness was determined for samples with a dECM concentration of 20 mg/mL. Adipose tissue-derived stromal cells (ASC) and a combination of ASC with human pulmonary microvascular endothelial cells (HPMVEC) were cultured, respectively, on and in hydrogels to analyze cellular plasticity in 2D and vascular network formation in 3D. Differentiation of ASC was induced with 10 ng/mL of TGF-beta 1 and SM22 alpha used as differentiation marker. 3D vascular network formation was evaluated with confocal microscopy after immunofluorescent staining of PECAM-1. In dECM, the most abundant protein was collagen VI for the left ventricle and mitral valve and elastin for the aorta. The stiffness of the hydrogel derived from the aorta (6,998 +/- 895 Pa) was significantly higher than those derived from the left ventricle (3,384 +/- 698 Pa) and the mitral valve (3,233 +/- 323 Pa) (One-way ANOVA,p= 0.0008). Aorta-derived dECM hydrogel drove non-induced (without TGF-beta 1) differentiation, while hydrogels derived from the left ventricle and mitral valve inhibited TGF-beta 1-induced differentiation. All hydrogels supported vascular network formation within 7 days of culture, but ventricular dECM hydrogel demonstrated more robust vascular networks, with thicker and longer vascular structures. All the three main cardiovascular tissues, myocardium, valves, and large arteries, could be used to fabricate hydrogels from dECM, and these showed an origin-dependent influence on ASC differentiation and vascular network formation.
Palavras-chave
hydrogels, extracellular matrix proteins, tissue engineering, regenerative medicine, biocompatible materials, biomimetic materials, tissue scaffolds, tissue decellularization
URI
https://observatorio.fm.usp.br/handle/OPI/36749
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCP
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/11