Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMACEDO, Sofia
dc.contributor.authorPESTANA, Ana
dc.contributor.authorSANTOS, Liliana
dc.contributor.authorNEVES, Celestino
dc.contributor.authorGUIMARAES, Susana
dc.contributor.authorDUARTE-NETO, Amaro
dc.contributor.authorDOLHNIKOFF, Marisa
dc.contributor.authorSALDIVA, Paulo
dc.contributor.authorALVES, Georgina
dc.contributor.authorOLIVEIRA, Rute
dc.contributor.authorCABANES, Didier
dc.contributor.authorCARNEIRO, Fatima
dc.contributor.authorSOBRINHO-SIMOES, Manuel
dc.contributor.authorSOARES, Paula
dc.date.accessioned2022-10-26T14:36:08Z
dc.date.available2022-10-26T14:36:08Z
dc.date.issued2022
dc.description.abstractObjective: To understand whether thyroid cells can be directly infected by the SARS-CoV-2 virus and to establish a putative correlation with the expression of the host entry machinery: ACE-2, TMPRSS2, and furin. Methods: We assessed the presence of SARS-CoV-2 virus at the gene level by RT-PCR, viral RNA transcripts localization by in situ hybridization, and by detecting viral proteins by immunohistochemistry for the nucleocapsid and the spike proteins. Furthermore, we also described the immunoexpression of key host factors for virus entry in the COVID-19 thyroid samples. Results: We performed RT-PCR for SARS-CoV-2 in all autopsy specimens and detected viral genome positivity in 13 of 15 thyroid tissues and in a lung specimen. In 9 of the 14 positive samples, we were also able to confirm SARS-CoV-2 signal by in situ hybridization. Immunohistochemistry for the viral nucleocapsid and spike protein was also positive for ten and nine of the RT-PCR-positive cases, respectively, but revealed a lower sensitivity. We also described, for the first time in a COVID-19 series, the immunohistochemical expression of ACE-2, TMPRSS2, and furin in the thyroid. Conclusions: Our results obtained in thyroid specimens from deceased COVID-19 patients indicate that thyrocytes can be directly infected by SARS-CoV-2 since we detected the presence of SARS-CoV-2 genome in follicular cells. Nevertheless, we did not find a clear correlation between the presence of viral genome and the expression of the host factors for virus entry, namely ACE-2, TMPRSS2, and furin.eng
dc.description.indexPubMedeng
dc.description.sponsorshipPortuguese funds through FCT [SFRH/BD/137802/2018]
dc.description.sponsorshipFCT through RESEARCH4COVID-19 Special Programme [625]
dc.description.sponsorshipPrograma Operacional Regional do Norte
dc.description.sponsorshipEuropean Regional Development Fund under the project 'The Porto Comprehensive Cancer Center' [NORTE-01-0145-FEDER-072678]
dc.identifier.citationEUROPEAN THYROID JOURNAL, v.11, n.4, article ID e220074, 10p, 2022
dc.identifier.doi10.1530/ETJ-22-0074
dc.identifier.eissn2235-0802
dc.identifier.issn2235-0640
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/49376
dc.language.isoeng
dc.publisherBIOSCIENTIFICA LTDeng
dc.relation.ispartofEuropean Thyroid Journal
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright BIOSCIENTIFICA LTDeng
dc.subjectSARS-CoV-2eng
dc.subjectthyroideng
dc.subjectACE-2eng
dc.subjectTMPRSS2eng
dc.subjectfurineng
dc.subject.otherreceptoreng
dc.subject.otherproteineng
dc.subject.otherace2eng
dc.subject.otherrnaeng
dc.subject.wosEndocrinology & Metabolismeng
dc.titleDetection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy serieseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryPortugal
hcfmusp.affiliation.countryisopt
hcfmusp.author.externalMACEDO, Sofia:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal; Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Inst Biomed Sci Abel Salazar, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal
hcfmusp.author.externalPESTANA, Ana:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal; Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal
hcfmusp.author.externalSANTOS, Liliana:Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal; North Lisbon Univ Hosp Ctr, Lisbon, Portugal
hcfmusp.author.externalNEVES, Celestino:Univ Porto, Fac Med, Porto, Portugal; Univ Hosp Ctr Sao Joao, Porto, Portugal
hcfmusp.author.externalGUIMARAES, Susana:Univ Porto, Fac Med, Porto, Portugal; Univ Hosp Ctr Sao Joao, Porto, Portugal
hcfmusp.author.externalALVES, Georgina:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal
hcfmusp.author.externalOLIVEIRA, Rute:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal
hcfmusp.author.externalCABANES, Didier:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal
hcfmusp.author.externalCARNEIRO, Fatima:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal; Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal; Univ Hosp Ctr Sao Joao, Porto, Portugal
hcfmusp.author.externalSOBRINHO-SIMOES, Manuel:Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal; Univ Hosp Ctr Sao Joao, Porto, Portugal
hcfmusp.author.externalSOARES, Paula:Univ Porto, Inst Res & Innovat Hlth, Porto, Portugal; Univ Porto, Inst Mol Pathol & Immunol, Porto, Portugal; Univ Porto, Fac Med, Porto, Portugal
hcfmusp.citation.scopus8
hcfmusp.contributor.author-fmusphcAMARO NUNES DUARTE NETO
hcfmusp.contributor.author-fmusphcMARISA DOLHNIKOFF
hcfmusp.contributor.author-fmusphcPAULO HILARIO NASCIMENTO SALDIVA
hcfmusp.description.articlenumbere220074
hcfmusp.description.issue4
hcfmusp.description.volume11
hcfmusp.origemWOS
hcfmusp.origem.pubmed35900859
hcfmusp.origem.scopus2-s2.0-85136097939
hcfmusp.origem.wosWOS:000848358900005
hcfmusp.publisher.cityBRISTOLeng
hcfmusp.publisher.countryENGLANDeng
hcfmusp.relation.referenceBrancatella A, 2020, J CLIN ENDOCR METAB, V105, P2367, DOI 10.1210/clinem/dgaa276eng
hcfmusp.relation.referenceChen M, 2021, THYROID, V31, P8, DOI 10.1089/thy.2020.0363eng
hcfmusp.relation.referenceChen WJ, 2021, ENDOCRINOLOGY, V162, DOI 10.1210/endocr/bqab004eng
hcfmusp.relation.referenceCoperchini F, 2021, ENDOCRINE, V74, P638, DOI 10.1007/s12020-021-02807-weng
hcfmusp.relation.referenceBeltrao FED, 2021, THYROID, V31, P1639, DOI 10.1089/thy.2021.0225eng
hcfmusp.relation.referenceHarris Asaf, 2021, AACE Clin Case Rep, V7, P14, DOI 10.1016/j.aace.2020.12.005eng
hcfmusp.relation.referenceJackson CB, 2022, NAT REV MOL CELL BIO, V23, P3, DOI 10.1038/s41580-021-00418-xeng
hcfmusp.relation.referenceKhailany RA, 2020, GENE REP, V19, DOI 10.1016/j.genrep.2020.100682eng
hcfmusp.relation.referenceKhoo B, 2021, J CLIN ENDOCR METAB, V106, pE803, DOI 10.1210/clinem/dgaa830eng
hcfmusp.relation.referenceLan J, 2020, NATURE, V581, P215, DOI 10.1038/s41586-020-2180-5eng
hcfmusp.relation.referenceLania A, 2020, EUR J ENDOCRINOL, V183, P381, DOI 10.1530/EJE-20-0335eng
hcfmusp.relation.referenceLeite JA, 2022, PLOS ONE, V17, DOI 10.1371/journal.pone.0252526eng
hcfmusp.relation.referenceLi WH, 2003, NATURE, V426, P450, DOI 10.1038/nature02145eng
hcfmusp.relation.referenceLiu YX, 2020, SCI CHINA LIFE SCI, V63, P364, DOI 10.1007/s11427-020-1643-8eng
hcfmusp.relation.referenceMeinhardt J, 2021, NAT NEUROSCI, V24, P168, DOI 10.1038/s41593-020-00758-5eng
hcfmusp.relation.referenceNuovo GJ, 2020, ANN DIAGN PATHOL, V48, DOI 10.1016/j.anndiagpath.2020.151565eng
hcfmusp.relation.referenceOrtega JT, 2020, EXCLI J, V19, P410, DOI 10.17179/excli2020-1167eng
hcfmusp.relation.referencePatel VB, 2016, CIRC RES, V118, P1313, DOI 10.1161/CIRCRESAHA.116.307708eng
hcfmusp.relation.referencePoma AM, 2021, THYROID, V31, P1766, DOI 10.1089/thy.2021.0345eng
hcfmusp.relation.referenceRotondi M, 2021, J ENDOCRINOL INVEST, V44, P1085, DOI 10.1007/s40618-020-01436-weng
hcfmusp.relation.referenceSalamanna F, 2020, FRONT MED-LAUSANNE, V7, DOI 10.3389/fmed.2020.594495eng
hcfmusp.relation.referenceSchaefer IM, 2020, MODERN PATHOL, V33, P2104, DOI 10.1038/s41379-020-0595-zeng
hcfmusp.relation.referenceShang J, 2020, P NATL ACAD SCI USA, V117, P11727, DOI 10.1073/pnas.2003138117eng
hcfmusp.relation.referencevan Lier D, 2021, ERJ OPEN RES, V7, DOI 10.1183/23120541.00848-2020eng
hcfmusp.relation.referenceWang F, 2012, J MOL DIAGN, V14, P22, DOI 10.1016/j.jmoldx.2011.08.002eng
hcfmusp.relation.referenceWang Xiao-Ming, 2021, Commun Med (Lond), V1, P24, DOI 10.1038/s43856-021-00025-zeng
hcfmusp.relation.referenceWang YL, 2020, INT J MED SCI, V17, P1522, DOI 10.7150/ijms.46695eng
hcfmusp.relation.referenceWong DWL, 2021, CELLS-BASEL, V10, DOI 10.3390/cells10081900eng
hcfmusp.relation.referenceZhou LL, 2020, ISCIENCE, V23, DOI 10.1016/j.isci.2020.101744eng
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Artigos e Materiais de Revistas Científicas - COVID-19
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