Identification of Leishmania species by next generation sequencing of hsp70 gene

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art_SOUZA_Identification_of_Leishmania_species_by_next_generation_sequencing_2022.PDF (3.12 MB)
Citações na Scopus
4
Tipo de produção
article
Data de publicação
2022
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Autores
TUON, Felipe Francisco
OLIVEIRA JUNIOR, Isael Gomes de
Citação
MOLECULAR AND CELLULAR PROBES, v.61, article ID 101791, 5p, 2022
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Leishmaniasis is a major public health problem worldwide. Although next generation sequencing technology has been widely used in the diagnosis of infectious diseases, it has been scarcely applied in identification of Leishmania species. The aim of this study was to compare the efficiency of MinIONTM nanopore sequencing and polymerase chain reaction restriction fragment length polymorphism in identifying Leishmania species. Our results showed that the MinIONTM sequencer was able to discriminate reference strains and clinical samples with high sensitivity in a cost and time effective manner without the prior need for culture.
Palavras-chave
Leishmaniasis, Heat shock protein 70 gene, Species, Nanopore, Next generation sequencing
URI
https://observatorio.fm.usp.br/handle/OPI/45977
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/32
Artigos e Materiais de Revistas Científicas - LIM/46
Artigos e Materiais de Revistas Científicas - LIM/49