Electrochemical Immunosensors Based on Zinc Oxide Nanorods for Detection of Antibodies Against SARS-CoV-2 Spike Protein in Convalescent and Vaccinated Individuals

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorNUNEZ, Freddy A.
dc.contributor.authorCASTRO, Ana C. H.
dc.contributor.authorOLIVEIRA, Vivian L. de
dc.contributor.authorLIMA, Ariane C.
dc.contributor.authorOLIVEIRA, Jamille R.
dc.contributor.authorMEDEIROS, Giuliana X. de
dc.contributor.authorSASAHARA, Greyce L.
dc.contributor.authorSANTOS, Keity S.
dc.contributor.authorLANFREDI, Alexandre J. C.
dc.contributor.authorALVES, Wendel A.
dc.date.accessioned2023-04-14T17:21:56Z
dc.date.available2023-04-14T17:21:56Z
dc.date.issued2023
dc.description.abstractEven after over 2 years of the COVID-19 pandemic, research on rapid, inexpensive, and accurate tests remains essential for controlling and avoiding the global spread of SARS-CoV-2 across the planet during a potential reappearance in future global waves or regional outbreaks. Assessment of serological responses for COVID-19 can be beneficial for population-level surveillance purposes, supporting the development of novel vaccines and evaluating the efficacy of different immunization programs. This can be especially relevant for broadly used inactivated whole virus vaccines, such as CoronaVac, which produced lower titers of neutralizing antibodies. and showed lower efficacy for specific groups such as the elderly and immunocompromised. We developed an impedimetric biosensor based on the immobilization of SARS-CoV-2 recombinant trimeric spike protein (S protein) on zinc oxide nanorod (ZnONR)-modified fluorine-doped tin oxide substrates for COVID-19 serology testing. Due to electrostatic interactions, the negatively charged S protein was immobilized via physical adsorption. The electrochemical response of the immunosensor was measured at each modification step and characterized by scanning electron microscopy and electrochemical techniques. We successfully evaluated the applicability of the modified ZnONR electrodes using serum samples from COVID-19 convalescent individuals, CoronaVac-vaccinated with or without positive results for SARS-CoV-2 infection, and pre-pandemic samples from healthy volunteers as controls. ELISA for IgG anti-SARS-CoV-2 spike protein was performed for comparison, and ELISA for IgG anti-RBDs of seasonal coronavirus (HCoVs) was used to test the specificity of immunosensor detection. No cross-reactivity with HCoVs was detected using the ZnONR immunosensor, and more interestingly, the sensor presented higher sensitivity when compared to negative ELISA results. The results demonstrate that the ZnONRs/spike-modified electrode displayed sensitive results for convalescents and vaccinated samples and shows excellent potential as a tool for the population's assessment and monitoring of seroconversion and seroprevalence.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.sponsorshipFAPESP [2017/02317-2]
dc.description.sponsorshipCNPq [465389/2014-7, 304389/2019-6, 407951/2021-0]
dc.description.sponsorshipCAPES [88881.504639/2020-01]
dc.description.sponsorshipNational Institute of Science and Technology in Bioanalytics [2014/50867-3]
dc.identifier.citationACS BIOMATERIALS SCIENCE & ENGINEERING, v.9, n.1, p.458-473, 2023
dc.identifier.doi10.1021/acsbiomaterials.2c00509
dc.identifier.issn2373-9878
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/52856
dc.language.isoeng
dc.publisherAMER CHEMICAL SOCeng
dc.relation.ispartofAcs Biomaterials Science & Engineering
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright AMER CHEMICAL SOCeng
dc.subjectseroprevalenceeng
dc.subjectCOVID-19eng
dc.subjectSARS-CoV-2eng
dc.subjectelectrochemical immunosensorseng
dc.subjectzinc oxideeng
dc.subjectserological diagnosiseng
dc.subjectimmunosurveillanceeng
dc.subjectCoronaVaceng
dc.subject.otherzno nanorodseng
dc.subject.otherimpedimetric biosensoreng
dc.subject.otherappropriate useeng
dc.subject.otherthin-filmseng
dc.subject.othernanoparticleseng
dc.subject.otherxpseng
dc.subject.otherphotoluminescenceeng
dc.subject.othergrowtheng
dc.subject.othernanostructureeng
dc.subject.othervalidationeng
dc.subject.wosMaterials Science, Biomaterialseng
dc.titleElectrochemical Immunosensors Based on Zinc Oxide Nanorods for Detection of Antibodies Against SARS-CoV-2 Spike Protein in Convalescent and Vaccinated Individualseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalNUNEZ, Freddy A.:Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, Brazil
hcfmusp.author.externalCASTRO, Ana C. H.:Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, Brazil
hcfmusp.author.externalOLIVEIRA, Vivian L. de:Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, Brazil
hcfmusp.author.externalLANFREDI, Alexandre J. C.:Univ Fed ABC, Ctr Engn, Modelagem & Ciencias Sociais Aplicadas, BR-09210580 Santo Andre, SP, Brazil
hcfmusp.author.externalALVES, Wendel A.:Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, Brazil
hcfmusp.citation.scopus15
hcfmusp.contributor.author-fmusphcARIANE CESARIO LIMA
hcfmusp.contributor.author-fmusphcJAMILLE RAMOS DE OLIVEIRA
hcfmusp.contributor.author-fmusphcGIULIANA XAVIER DE MEDEIROS
hcfmusp.contributor.author-fmusphcGREYCE LURI SASAHARA
hcfmusp.contributor.author-fmusphcKEITY SOUZA SANTOS
hcfmusp.description.beginpage458
hcfmusp.description.endpage473
hcfmusp.description.issue1
hcfmusp.description.volume9
hcfmusp.origemWOS
hcfmusp.origem.pubmed36048716
hcfmusp.origem.scopus2-s2.0-85137858125
hcfmusp.origem.wosWOS:000862813500001
hcfmusp.publisher.cityWASHINGTONeng
hcfmusp.publisher.countryUSAeng
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