Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPEREIRA, Natalli Zanete
dc.contributor.authorCARDOSO, Elaine Cristina
dc.contributor.authorOLIVEIRA, Luanda Mara da Silva
dc.contributor.authorLIMA, Josenilson Feitosa de
dc.contributor.authorBRANCO, Anna Claudia Calvielli Castelo
dc.contributor.authorRUOCCO, Rosa Maria de Souza Aveiro
dc.contributor.authorZUGAIB, Marcelo
dc.contributor.authorOLIVEIRA FILHO, Joao Bosco de
dc.contributor.authorDUARTE, Alberto Jose da Silva
dc.contributor.authorSATO, Maria Notomi
dc.date.accessioned2014-04-25T21:53:08Z
dc.date.available2014-04-25T21:53:08Z
dc.date.issued2013
dc.description.abstractPrograms for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy (ART) in pregnancy (n=23) and in cord blood (CB) (n=16), placental tissues (n=10-13) and colostrum (n=5-6) samples and compared them to expression in samples from uninfected (UN) pregnant mothers (n=21). Mononuclear cells (MNCs) were prepared from maternal and CB samples following deliveries by cesarean section. Maternal (decidua) and fetal (chorionic villus) placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G (A3G), A3F, tripartite motif family-5 alpha (TRIM-5 alpha), TRIM-22, myxovirus resistance protein A (MxA), stimulator of interferon (IFN) genes (STING) and IFN-beta, compared with the levels detected in uninfected (UN) mother-CB pairs. Moreover, A3G transcript and protein levels and alpha-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5 alpha protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected (UN) mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-beta mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2008/55962-3, 2012/18837-1]
dc.description.sponsorshipLaboratorio de Investigacao Medica, Unidade 56 do Hospital das Clinicas da Faculdade de Medicina de Sao Paulo (FMUSP-HC/LIM-56)
dc.identifier.citationPLOS ONE, v.8, n.12, article ID e84917, 11p, 2013
dc.identifier.doi10.1371/journal.pone.0084917
dc.identifier.issn1932-6203
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5142
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPlos One
dc.rightsopenAccess
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCE
dc.subject.otherimmune-response
dc.subject.otherdisease progression
dc.subject.otheruninfected infants
dc.subject.otherinfluenza-virus
dc.subject.otherediting enzyme
dc.subject.otherbreast-milk
dc.subject.otherapobec3g
dc.subject.othercells
dc.subject.othertransmission
dc.subject.otherprotein
dc.subject.wosMultidisciplinary Sciences
dc.titleUpregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalPEREIRA, Natalli Zanete:Univ Sao Paulo, Sch Med, Lab Dermatol & Immunodeficiencies, LIM 56,Dept Dermatol,Trop Med Inst Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalCARDOSO, Elaine Cristina:Univ Sao Paulo, Sch Med, Lab Dermatol & Immunodeficiencies, LIM 56,Dept Dermatol,Trop Med Inst Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalBRANCO, Anna Claudia Calvielli Castelo:Univ Sao Paulo, Sch Med, Lab Dermatol & Immunodeficiencies, LIM 56,Dept Dermatol,Trop Med Inst Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalOLIVEIRA FILHO, Joao Bosco de:Med Inst Prof Fernando Figueira IMIP, Dept Pediat, Recife, PE, Brazil
hcfmusp.citation.scopus14
hcfmusp.contributor.author-fmusphcLUANDA MARA DA SILVA OLIVEIRA
hcfmusp.contributor.author-fmusphcJOSENILSON FEITOSA DE LIMA
hcfmusp.contributor.author-fmusphcROSA MARIA DE SOUZA AVEIRO RUOCCO
hcfmusp.contributor.author-fmusphcMARCELO ZUGAIB
hcfmusp.contributor.author-fmusphcALBERTO JOSE DA SILVA DUARTE
hcfmusp.contributor.author-fmusphcMARIA NOTOMI SATO
hcfmusp.description.articlenumbere84917
hcfmusp.description.issue12
hcfmusp.description.volume8
hcfmusp.origemWOS
hcfmusp.origem.pubmed24367701
hcfmusp.origem.scopus2-s2.0-84893162418
hcfmusp.origem.wosWOS:000328740300159
hcfmusp.publisher.citySAN FRANCISCO
hcfmusp.publisher.countryUSA
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hcfmusp.remissive.sponsorshipFAPESP
hcfmusp.remissive.sponsorshipFMUSP-HC
hcfmusp.scopus.lastupdate2024-05-10
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