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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | BRANDT, Katia | - |
dc.contributor.author | TADDEI, Carla R. | - |
dc.contributor.author | TAKAGI, Elizabeth H. | - |
dc.contributor.author | OLIVEIRA, Fernanda F. | - |
dc.contributor.author | DUARTE, Rubens T. D. | - |
dc.contributor.author | IRINO, Isabel | - |
dc.contributor.author | MARTINEZ, Marina B. | - |
dc.contributor.author | CARNEIRO-SAMPAIO, Magda | - |
dc.date.accessioned | 2013-07-30T15:18:23Z | - |
dc.date.available | 2013-07-30T15:18:23Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | CLINICS, v.67, n.2, p.113-123, 2012 | - |
dc.identifier.issn | 1807-5932 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/1030 | - |
dc.description.abstract | OBJECTIVE: The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of Sao Paulo, Brazil, during the first month of life, was studied. METHODS: Fecal samples were collected from ten neonates on the second, seventh, and 30th days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30th day to amplify DNA from Bifidobacterium sp. RESULTS: The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30th day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. CONCLUSION: The observed pattern of Escherichia predominance and reduced Staphylococcus colonization is in contrast with the patterns observed in neonates living in developed countries. | - |
dc.language.iso | eng | - |
dc.publisher | HOSPITAL CLINICAS, UNIV SAO PAULO | - |
dc.relation.ispartof | Clinics | - |
dc.rights | openAccess | - |
dc.subject | Microbiota | - |
dc.subject | Breastfeeding | - |
dc.subject | 16S rRNA | - |
dc.subject | Escherichia | - |
dc.subject | Clostridium | - |
dc.subject.other | intestinal microbiota | - |
dc.subject.other | escherichia-coli | - |
dc.subject.other | gut microbiota | - |
dc.subject.other | clostridium-difficile | - |
dc.subject.other | antibiotic-treatment | - |
dc.subject.other | sequence data | - |
dc.subject.other | fed infants | - |
dc.subject.other | colonization | - |
dc.subject.other | flora | - |
dc.subject.other | children | - |
dc.title | Establishment of the bacterial fecal community during the first month of life in Brazilian newborns | - |
dc.type | article | - |
dc.rights.holder | Copyright HOSPITAL CLINICAS, UNIV SAO PAULO | - |
dc.identifier.doi | 10.6061/clinics/2012(02)05 | - |
dc.identifier.pmid | 22358235 | - |
dc.subject.wos | Medicine, General & Internal | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | BRANDT, Katia:Univ Sao Paulo, Sch Med, Hosp Clin, Childrens Hosp, Sao Paulo, Brazil; Inst Med Integral Prof Fernando Figueira, Recife, PE, Brazil | - |
hcfmusp.author.external | TADDEI, Carla R.:Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil; Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, Brazil | - |
hcfmusp.author.external | TAKAGI, Elizabeth H.:Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil | - |
hcfmusp.author.external | OLIVEIRA, Fernanda F.:Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil | - |
hcfmusp.author.external | DUARTE, Rubens T. D.:Univ Sao Paulo, Microbial Ecol Lab, Oceanog Inst, Sao Paulo, Brazil | - |
hcfmusp.author.external | IRINO, Isabel:Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil | - |
hcfmusp.author.external | MARTINEZ, Marina B.:Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 113 | - |
hcfmusp.description.endpage | 123 | - |
hcfmusp.description.issue | 2 | - |
hcfmusp.description.volume | 67 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84857683553 | - |
hcfmusp.origem.id | WOS:000300664500005 | - |
hcfmusp.origem.id | SCIELO:S1807-59322012000200005 | - |
hcfmusp.publisher.city | SAO PAULO | - |
hcfmusp.publisher.country | BRAZIL | - |
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dc.description.index | MEDLINE | - |
hcfmusp.lim.ref | 2012 | - |
hcfmusp.citation.scopus | 18 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPE Artigos e Materiais de Revistas Científicas - HC/ICr Artigos e Materiais de Revistas Científicas - LIM/36 |
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